Immunomodulatory roles and functional analysis of pre-B lymphocyte DT40 cells with the bursal-derived BSP-II treatment

Xiuli Feng, Bin Zhou, Rui Bing Cao, Qing Tao Liu, Ke Liu, Xiao Dong Liu, Yuan Peng Zhang, Li Huang, Xiang Bo Ji, Jun Luo, Gaiping Zhang, Pu Yan Chen

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


The bursa of Fabricius, the acknowledged central humoral immune organ, is vital to B cell differentiation. However, the regulatory function of the bursal-derived peptide on avian B cell proliferation has not been reported. BSP-II is a recently reported bursal-derived bioactive peptide. In this paper, 75 days-old chicks were twice subcutaneously immunized with BSP-II and inactivated avian influenza virus (AIV, H9N2 strain). It was proved that BSP-II induced a strongly AIV-specific HI antibody production in the immunized chicks. Also, BSP-II could enhance avian pre-B lymphocyte DT40 cell viability. To investigate the global patterns of gene expression in DT40 cells after BSP-II treatment, gene microarray was carried out. It was identified that the differentially expressed genes were involved in various pathways, of which six pathways were associated with signaling transductions, including ErbB signaling, MAPK signaling, Toll-like receptor signaling, Notch signaling, mTOR signaling, and Wnt signaling. Finally, RT-qPCR was used to confirm the microarray expression data. These results indicated the molecular basis of pre-B lymphocyte viability with BSP-II treatment, which provided a potential mechanism of the bursa of Fabricius on pre-B lymphocyte viability, differentiation, and development. These results are valid for the mechanism of the bursa of Fabricius on B lymphocytes development.

Original languageEnglish (US)
Pages (from-to)292-298
Number of pages7
Issue number2
StatePublished - Aug 1 2012
Externally publishedYes


  • Bursal-derived BSP-II
  • Functional analysis
  • Gene microarray
  • Immunization experiment
  • Pre-B lymphocyte DT40 cell

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience


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