TY - JOUR
T1 - Immunomodulatory roles and functional analysis of pre-B lymphocyte DT40 cells with the bursal-derived BSP-II treatment
AU - Feng, Xiuli
AU - Zhou, Bin
AU - Cao, Rui Bing
AU - Liu, Qing Tao
AU - Liu, Ke
AU - Liu, Xiao Dong
AU - Zhang, Yuan Peng
AU - Huang, Li
AU - Ji, Xiang Bo
AU - Luo, Jun
AU - Zhang, Gaiping
AU - Chen, Pu Yan
PY - 2012/8/1
Y1 - 2012/8/1
N2 - The bursa of Fabricius, the acknowledged central humoral immune organ, is vital to B cell differentiation. However, the regulatory function of the bursal-derived peptide on avian B cell proliferation has not been reported. BSP-II is a recently reported bursal-derived bioactive peptide. In this paper, 75 days-old chicks were twice subcutaneously immunized with BSP-II and inactivated avian influenza virus (AIV, H9N2 strain). It was proved that BSP-II induced a strongly AIV-specific HI antibody production in the immunized chicks. Also, BSP-II could enhance avian pre-B lymphocyte DT40 cell viability. To investigate the global patterns of gene expression in DT40 cells after BSP-II treatment, gene microarray was carried out. It was identified that the differentially expressed genes were involved in various pathways, of which six pathways were associated with signaling transductions, including ErbB signaling, MAPK signaling, Toll-like receptor signaling, Notch signaling, mTOR signaling, and Wnt signaling. Finally, RT-qPCR was used to confirm the microarray expression data. These results indicated the molecular basis of pre-B lymphocyte viability with BSP-II treatment, which provided a potential mechanism of the bursa of Fabricius on pre-B lymphocyte viability, differentiation, and development. These results are valid for the mechanism of the bursa of Fabricius on B lymphocytes development.
AB - The bursa of Fabricius, the acknowledged central humoral immune organ, is vital to B cell differentiation. However, the regulatory function of the bursal-derived peptide on avian B cell proliferation has not been reported. BSP-II is a recently reported bursal-derived bioactive peptide. In this paper, 75 days-old chicks were twice subcutaneously immunized with BSP-II and inactivated avian influenza virus (AIV, H9N2 strain). It was proved that BSP-II induced a strongly AIV-specific HI antibody production in the immunized chicks. Also, BSP-II could enhance avian pre-B lymphocyte DT40 cell viability. To investigate the global patterns of gene expression in DT40 cells after BSP-II treatment, gene microarray was carried out. It was identified that the differentially expressed genes were involved in various pathways, of which six pathways were associated with signaling transductions, including ErbB signaling, MAPK signaling, Toll-like receptor signaling, Notch signaling, mTOR signaling, and Wnt signaling. Finally, RT-qPCR was used to confirm the microarray expression data. These results indicated the molecular basis of pre-B lymphocyte viability with BSP-II treatment, which provided a potential mechanism of the bursa of Fabricius on pre-B lymphocyte viability, differentiation, and development. These results are valid for the mechanism of the bursa of Fabricius on B lymphocytes development.
KW - Bursal-derived BSP-II
KW - Functional analysis
KW - Gene microarray
KW - Immunization experiment
KW - Pre-B lymphocyte DT40 cell
UR - http://www.scopus.com/inward/record.url?scp=84864147386&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84864147386&partnerID=8YFLogxK
U2 - 10.1016/j.peptides.2012.04.015
DO - 10.1016/j.peptides.2012.04.015
M3 - Article
C2 - 22561065
AN - SCOPUS:84864147386
VL - 36
SP - 292
EP - 298
JO - Peptides
JF - Peptides
SN - 0196-9781
IS - 2
ER -