Immunological identification and characterization of a delayed rectifier K+ channel polypeptide in rat brain

James Trimmer

Research output: Contribution to journalArticle

184 Citations (Scopus)

Abstract

Antibodies specific for the drk1 polypeptide were used to characterize the corresponding protein in rat brain. Recombinant and synthetic immunogens containing fragments of the drk1 polypeptide were produced. Antibodies raised to these immunogens display monospecific reactions with the same 130-kDa polypeptide on immunoblots of adult rat brain membranes. Immunoprecipitation of 125I-labeled brain membranes identifies a 38-kDa peptide in tight association with the drk1 polypeptide. Immunohistochemical staining of sections of adult rat cortex shows that drk1 protein is restricted to neurons, where staining is present on dendrites and cell bodies but not on axons. These studies point to the value of such immunological reagents to the further characterization of the components of this delayed rectifier K+ channel in the mammalian central nervous system.

Original languageEnglish (US)
Pages (from-to)10764-10768
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number23
StatePublished - 1991
Externally publishedYes

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Peptides
Brain
Staining and Labeling
Synthetic Vaccines
Membranes
Antibodies
Dendrites
Immunoprecipitation
Axons
Proteins
Central Nervous System
Neurons

Keywords

  • Excitability
  • Ion-channel molecular biology
  • Mammalian central nervous system
  • Tissue-specific gene expression

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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AB - Antibodies specific for the drk1 polypeptide were used to characterize the corresponding protein in rat brain. Recombinant and synthetic immunogens containing fragments of the drk1 polypeptide were produced. Antibodies raised to these immunogens display monospecific reactions with the same 130-kDa polypeptide on immunoblots of adult rat brain membranes. Immunoprecipitation of 125I-labeled brain membranes identifies a 38-kDa peptide in tight association with the drk1 polypeptide. Immunohistochemical staining of sections of adult rat cortex shows that drk1 protein is restricted to neurons, where staining is present on dendrites and cell bodies but not on axons. These studies point to the value of such immunological reagents to the further characterization of the components of this delayed rectifier K+ channel in the mammalian central nervous system.

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