TY - JOUR
T1 - Immunological equivalence between mouse brain-derived and Vero cell-derived Japanese encephalitis vaccines
AU - Abe, Motoharu
AU - Shiosaki, Kouichi
AU - Hammar, Lena
AU - Sonoda, Kengo
AU - Xing, Li
AU - Kuzuhara, Syoji
AU - Kino, Yoichiro
AU - Cheng, R. Holland
PY - 2006/11
Y1 - 2006/11
N2 - The persistent spread via animal reservoirs urges expanding vaccination programs against pathogens like the Japanese encephalitis virus, JEV. The JEV is spreads to new areas by domestic as well as by wild animals. Although there is a safe and efficient vaccine on the market, this is derived from infected mouse brains, why today's situation requires overcoming the potential risk caused by using animal tissues. To meet this demand we have developed a Vero cell-derived JEV vaccine, using the same virus strain as in the established one. A phase III clinical study of the new vaccine has recently been completed with positive outcome. Like the established mouse brain-derived vaccine, the Vero cell-derived one is a formalin inactivated whole virus vaccine. We here demonstrate the very good agreement in immunological tests between the two antigens. The study includes analyses with two neutralizing monoclonal antibodies that blocks cell entry at a late stage in infection, assumedly interfering with fusion-related refolding in the virus fusion protein. It is obvious that the formalin inactivation treatment, with both virus preparations, retains these essential vaccine epitopes.
AB - The persistent spread via animal reservoirs urges expanding vaccination programs against pathogens like the Japanese encephalitis virus, JEV. The JEV is spreads to new areas by domestic as well as by wild animals. Although there is a safe and efficient vaccine on the market, this is derived from infected mouse brains, why today's situation requires overcoming the potential risk caused by using animal tissues. To meet this demand we have developed a Vero cell-derived JEV vaccine, using the same virus strain as in the established one. A phase III clinical study of the new vaccine has recently been completed with positive outcome. Like the established mouse brain-derived vaccine, the Vero cell-derived one is a formalin inactivated whole virus vaccine. We here demonstrate the very good agreement in immunological tests between the two antigens. The study includes analyses with two neutralizing monoclonal antibodies that blocks cell entry at a late stage in infection, assumedly interfering with fusion-related refolding in the virus fusion protein. It is obvious that the formalin inactivation treatment, with both virus preparations, retains these essential vaccine epitopes.
KW - Cell system derived whole virus vaccine
KW - Fusion protein refolding
KW - Japanese encephalitis
KW - Neutralizing antibodies
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U2 - 10.1016/j.virusres.2006.05.004
DO - 10.1016/j.virusres.2006.05.004
M3 - Article
C2 - 16815584
AN - SCOPUS:33748940413
VL - 121
SP - 152
EP - 160
JO - Virus Research
JF - Virus Research
SN - 0168-1702
IS - 2
ER -