Immunologic characterization of oligodendroglia

O. Abramsky; R. P. Lisak; David E Pleasure; D. H. Gilden; D. H. Silberberg

doi:10.1016/0304-3940(78)90141-6

Immunologic characterization of oligodendroglia

O. Abramsky, R. P. Lisak, David E Pleasure, D. H. Gilden, D. H. Silberberg

Research output: Contribution to journal › Article

10 Scopus citations

Abstract

Oligodendrocytes were isolated from bovine white matter by trypsinization and sucrose gradient centrifugation, and were injected into rabbits. Indirect immunofluorescence studies showed that antisera reacted specifically with the surface of isolated oligodendrocytes in suspension, as well as with perineural and interfasicular oligodendroglia in bovine and human brain sections. This activity was blocked by pre-incubation of cells or brain sections with non-fluoresceinated goat anti-rabbit immunoglobulin, and absorbed by oligodendrocytes or white matter, but not by purified myelin, neuroblastoma or liver.

Original language	English (US)
Pages (from-to)	311-316
Number of pages	6
Journal	Neuroscience Letters
Volume	8
Issue number	4
DOIs	https://doi.org/10.1016/0304-3940(78)90141-6
State	Published - 1978
Externally published	Yes

ASJC Scopus subject areas

Neuroscience(all)

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title = "Immunologic characterization of oligodendroglia",

abstract = "Oligodendrocytes were isolated from bovine white matter by trypsinization and sucrose gradient centrifugation, and were injected into rabbits. Indirect immunofluorescence studies showed that antisera reacted specifically with the surface of isolated oligodendrocytes in suspension, as well as with perineural and interfasicular oligodendroglia in bovine and human brain sections. This activity was blocked by pre-incubation of cells or brain sections with non-fluoresceinated goat anti-rabbit immunoglobulin, and absorbed by oligodendrocytes or white matter, but not by purified myelin, neuroblastoma or liver.",

author = "O. Abramsky and Lisak, {R. P.} and Pleasure, {David E} and Gilden, {D. H.} and Silberberg, {D. H.}",

year = "1978",

doi = "10.1016/0304-3940(78)90141-6",

language = "English (US)",

volume = "8",

pages = "311--316",

journal = "Neuroscience Letters",

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T1 - Immunologic characterization of oligodendroglia

AU - Abramsky, O.

AU - Lisak, R. P.

AU - Pleasure, David E

AU - Gilden, D. H.

AU - Silberberg, D. H.

PY - 1978

Y1 - 1978

N2 - Oligodendrocytes were isolated from bovine white matter by trypsinization and sucrose gradient centrifugation, and were injected into rabbits. Indirect immunofluorescence studies showed that antisera reacted specifically with the surface of isolated oligodendrocytes in suspension, as well as with perineural and interfasicular oligodendroglia in bovine and human brain sections. This activity was blocked by pre-incubation of cells or brain sections with non-fluoresceinated goat anti-rabbit immunoglobulin, and absorbed by oligodendrocytes or white matter, but not by purified myelin, neuroblastoma or liver.

AB - Oligodendrocytes were isolated from bovine white matter by trypsinization and sucrose gradient centrifugation, and were injected into rabbits. Indirect immunofluorescence studies showed that antisera reacted specifically with the surface of isolated oligodendrocytes in suspension, as well as with perineural and interfasicular oligodendroglia in bovine and human brain sections. This activity was blocked by pre-incubation of cells or brain sections with non-fluoresceinated goat anti-rabbit immunoglobulin, and absorbed by oligodendrocytes or white matter, but not by purified myelin, neuroblastoma or liver.

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EP - 316

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

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