Abstract
Oligodendrocytes were isolated from bovine white matter by trypsinization and sucrose gradient centrifugation, and were injected into rabbits. Indirect immunofluorescence studies showed that antisera reacted specifically with the surface of isolated oligodendrocytes in suspension, as well as with perineural and interfasicular oligodendroglia in bovine and human brain sections. This activity was blocked by pre-incubation of cells or brain sections with non-fluoresceinated goat anti-rabbit immunoglobulin, and absorbed by oligodendrocytes or white matter, but not by purified myelin, neuroblastoma or liver.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 311-316 |
| Number of pages | 6 |
| Journal | Neuroscience Letters |
| Volume | 8 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1978 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Immunologic characterization of oligodendroglia. / Abramsky, O.; Lisak, R. P.; Pleasure, David E; Gilden, D. H.; Silberberg, D. H.
In: Neuroscience Letters, Vol. 8, No. 4, 1978, p. 311-316.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunologic characterization of oligodendroglia
AU - Abramsky, O.
AU - Lisak, R. P.
AU - Pleasure, David E
AU - Gilden, D. H.
AU - Silberberg, D. H.
PY - 1978
Y1 - 1978
N2 - Oligodendrocytes were isolated from bovine white matter by trypsinization and sucrose gradient centrifugation, and were injected into rabbits. Indirect immunofluorescence studies showed that antisera reacted specifically with the surface of isolated oligodendrocytes in suspension, as well as with perineural and interfasicular oligodendroglia in bovine and human brain sections. This activity was blocked by pre-incubation of cells or brain sections with non-fluoresceinated goat anti-rabbit immunoglobulin, and absorbed by oligodendrocytes or white matter, but not by purified myelin, neuroblastoma or liver.
AB - Oligodendrocytes were isolated from bovine white matter by trypsinization and sucrose gradient centrifugation, and were injected into rabbits. Indirect immunofluorescence studies showed that antisera reacted specifically with the surface of isolated oligodendrocytes in suspension, as well as with perineural and interfasicular oligodendroglia in bovine and human brain sections. This activity was blocked by pre-incubation of cells or brain sections with non-fluoresceinated goat anti-rabbit immunoglobulin, and absorbed by oligodendrocytes or white matter, but not by purified myelin, neuroblastoma or liver.
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UR - http://www.scopus.com/inward/citedby.url?scp=0018194573&partnerID=8YFLogxK
U2 - 10.1016/0304-3940(78)90141-6
DO - 10.1016/0304-3940(78)90141-6
M3 - Article
VL - 8
SP - 311
EP - 316
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 4
ER -