Immunolocalization of steroidogenic enzymes in the vaginal mucous of Galea spixii during the estrous cycle

Amilton Cesar dos Santos, Alan J Conley, Moacir Franco de Oliveira, Gleidson Benevides Oliveira, Diego Carvalho Viana, Antônio Chaves de Assis Neto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: The synthesis of sex steroids is controlled by several enzymes such as17α-hydroxylase cytochrome P450 (P450c17) catalyzing androgen synthesis and aromatase cytochrome P450 (P450arom) catalyzing estrogen synthesis, both of which must complex with the redox partner NADPH-cytochrome P450 oxidoreductase (CPR) for activity. Previous studies have identified expression of steroidogenic enzymes in vaginal tissue, suggesting local sex steroid synthesis. The current studies investigate P450c17, P450aromatase and CPR expression in vaginal mucosa of Galea spixii (Spix cavy) by immuno-histochemical and western immunoblot analyses. Methods: Stages of estrous cyclicity were monitored by vaginal exfoliative cytology. After euthanasia, vaginal tissues were retrieved, fixed and frozen at diestrus, proestrus, estrus and metestrus. The ovaries and testis were used as positive control tissues for immunohistochemistry. Results: Data from cytological study allowed identification of different estrous cycle phases. Immunohistochemical analysis showed different sites of expression of steroidogenic enzymes along with tissue response throughout different phases of the estrous cycle. However, further studies are needed to characterize the derived hormones synthesized by, and the enzymes activities associated with, vaginal tissues. Conclusion: Current results not only support the expression of enzymes involved in sex steroid synthesis in the wall of the vagina, they also indicate that expression changes with the stage of the cycle, both the levels and types of cells exhibiting expression. Thus, changes in proliferation of vaginal epithelial cells and the differentiation of the mucosa may be influenced by local steroid synthesis as well as circulating androgens and estrogens.

Original languageEnglish (US)
Article number30
JournalReproductive Biology and Endocrinology
Volume15
Issue number1
DOIs
StatePublished - Apr 24 2017

Fingerprint

Estrous Cycle
Steroids
Cytochrome P-450 Enzyme System
Enzymes
Androgens
Estrogens
Mucous Membrane
Metestrus
Diestrus
Proestrus
NADPH-Ferrihemoprotein Reductase
Aromatase
Euthanasia
Estrus
Vagina
Periodicity
Mixed Function Oxygenases
Oxidation-Reduction
Cell Biology
Testis

Keywords

  • Androgens
  • Cytochrome P450
  • Estrogens
  • Estrous cycle
  • Vaginal epithelium

ASJC Scopus subject areas

  • Reproductive Medicine
  • Endocrinology
  • Developmental Biology

Cite this

Immunolocalization of steroidogenic enzymes in the vaginal mucous of Galea spixii during the estrous cycle. / dos Santos, Amilton Cesar; Conley, Alan J; de Oliveira, Moacir Franco; Oliveira, Gleidson Benevides; Viana, Diego Carvalho; de Assis Neto, Antônio Chaves.

In: Reproductive Biology and Endocrinology, Vol. 15, No. 1, 30, 24.04.2017.

Research output: Contribution to journalArticle

dos Santos, Amilton Cesar ; Conley, Alan J ; de Oliveira, Moacir Franco ; Oliveira, Gleidson Benevides ; Viana, Diego Carvalho ; de Assis Neto, Antônio Chaves. / Immunolocalization of steroidogenic enzymes in the vaginal mucous of Galea spixii during the estrous cycle. In: Reproductive Biology and Endocrinology. 2017 ; Vol. 15, No. 1.
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AB - Background: The synthesis of sex steroids is controlled by several enzymes such as17α-hydroxylase cytochrome P450 (P450c17) catalyzing androgen synthesis and aromatase cytochrome P450 (P450arom) catalyzing estrogen synthesis, both of which must complex with the redox partner NADPH-cytochrome P450 oxidoreductase (CPR) for activity. Previous studies have identified expression of steroidogenic enzymes in vaginal tissue, suggesting local sex steroid synthesis. The current studies investigate P450c17, P450aromatase and CPR expression in vaginal mucosa of Galea spixii (Spix cavy) by immuno-histochemical and western immunoblot analyses. Methods: Stages of estrous cyclicity were monitored by vaginal exfoliative cytology. After euthanasia, vaginal tissues were retrieved, fixed and frozen at diestrus, proestrus, estrus and metestrus. The ovaries and testis were used as positive control tissues for immunohistochemistry. Results: Data from cytological study allowed identification of different estrous cycle phases. Immunohistochemical analysis showed different sites of expression of steroidogenic enzymes along with tissue response throughout different phases of the estrous cycle. However, further studies are needed to characterize the derived hormones synthesized by, and the enzymes activities associated with, vaginal tissues. Conclusion: Current results not only support the expression of enzymes involved in sex steroid synthesis in the wall of the vagina, they also indicate that expression changes with the stage of the cycle, both the levels and types of cells exhibiting expression. Thus, changes in proliferation of vaginal epithelial cells and the differentiation of the mucosa may be influenced by local steroid synthesis as well as circulating androgens and estrogens.

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