TY - JOUR
T1 - Immunolocalization of IL-17A, IL-17B, and their receptors in chondrocytes during fracture healing
AU - Kokubu, Takeshi
AU - Haudenschild, Dominik R
AU - Moseley, Timothy A.
AU - Rose, Larry
AU - Reddi, A Hari
PY - 2008/2
Y1 - 2008/2
N2 - Fracture healing in long bones is a sequential multistep cascade of hemostasis, transient inflammation, chemotaxis of progenitor cells, mitosis, differentiation of cartilage, and replacement with bone. This multistep cascade is orchestrated by cytokines and morphogens. Members of the interleukin (IL)-17 family, including IL-17B, have been identified in cartilage, but their expression during fracture healing is unknown. In this study, we determined the immunolocalization of cytokines IL-17A and IL-17B, along with the IL-17 receptor (IL-17R) and IL-17 receptor-like protein (IL-17RL), during the sequence of fracture repair in a standard model. The results were extended to developmental changes in the epiphyseal growth plate of long bones. Members of the IL-17 family were localized in chondrocytes in the fracture callus. Moreover, we found significant parallels to the localization of these cytokines and their receptors in chondrocytes during an endochondral differentiation program in the epiphyseal growth plate.
AB - Fracture healing in long bones is a sequential multistep cascade of hemostasis, transient inflammation, chemotaxis of progenitor cells, mitosis, differentiation of cartilage, and replacement with bone. This multistep cascade is orchestrated by cytokines and morphogens. Members of the interleukin (IL)-17 family, including IL-17B, have been identified in cartilage, but their expression during fracture healing is unknown. In this study, we determined the immunolocalization of cytokines IL-17A and IL-17B, along with the IL-17 receptor (IL-17R) and IL-17 receptor-like protein (IL-17RL), during the sequence of fracture repair in a standard model. The results were extended to developmental changes in the epiphyseal growth plate of long bones. Members of the IL-17 family were localized in chondrocytes in the fracture callus. Moreover, we found significant parallels to the localization of these cytokines and their receptors in chondrocytes during an endochondral differentiation program in the epiphyseal growth plate.
KW - Bone
KW - Bone development
KW - Chondrocyte
KW - Fracture healing
KW - Interleukin-17 receptor-like molecule
KW - Interleukin-17 receptor-like protein
KW - Interleukin-17B
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UR - http://www.scopus.com/inward/citedby.url?scp=38649100002&partnerID=8YFLogxK
U2 - 10.1369/jhc.7A7223.2007
DO - 10.1369/jhc.7A7223.2007
M3 - Article
C2 - 17827167
AN - SCOPUS:38649100002
VL - 56
SP - 89
EP - 95
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
SN - 0022-1554
IS - 2
ER -