Abstract
Expression of bovine leukemia virus (BLV) antigens in vivo has not been shown. After BLV infection, however, production of antibodies directed towards BLV proteins (e.g. gp51) can be easily demonstrated. Thus, production of BLV proteins has to take place somewhere in infected cattle. Tissues and organs of experimentally infected cattle were fixed in acetone and embedded in paraffin. Monoclonal antibodies directed to gp51 were used to demonstrate BLV expression immunohistologically by the peroxidase-antiperoxidase (PAP) method. The same samples were also used to demonstrate a tumor associated antigen (TAA) employing a monoclonal antibody. Our results indicate that very few cells, found in the intestinal mucosa, produce gp51 in vivo. The expression of TAA, however, increases significantly shortly after infection with BLV and remains high throughout life.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 223-231 |
| Number of pages | 9 |
| Journal | Veterinary Immunology and Immunopathology |
| Volume | 22 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 1 1989 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
- veterinary(all)
Cite this
Immunohistological demonstration of virus and tumor associated antigens in tissues in experimental and spontaneous Bovine leukemia virus (BLV) infection. / Reinacher, M.; Thurmond, Mark; Onuma, M.; Portetelle, D.; Picanso, J.; Theilen, G. H.
In: Veterinary Immunology and Immunopathology, Vol. 22, No. 3, 01.01.1989, p. 223-231.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunohistological demonstration of virus and tumor associated antigens in tissues in experimental and spontaneous Bovine leukemia virus (BLV) infection
AU - Reinacher, M.
AU - Thurmond, Mark
AU - Onuma, M.
AU - Portetelle, D.
AU - Picanso, J.
AU - Theilen, G. H.
PY - 1989/1/1
Y1 - 1989/1/1
N2 - Expression of bovine leukemia virus (BLV) antigens in vivo has not been shown. After BLV infection, however, production of antibodies directed towards BLV proteins (e.g. gp51) can be easily demonstrated. Thus, production of BLV proteins has to take place somewhere in infected cattle. Tissues and organs of experimentally infected cattle were fixed in acetone and embedded in paraffin. Monoclonal antibodies directed to gp51 were used to demonstrate BLV expression immunohistologically by the peroxidase-antiperoxidase (PAP) method. The same samples were also used to demonstrate a tumor associated antigen (TAA) employing a monoclonal antibody. Our results indicate that very few cells, found in the intestinal mucosa, produce gp51 in vivo. The expression of TAA, however, increases significantly shortly after infection with BLV and remains high throughout life.
AB - Expression of bovine leukemia virus (BLV) antigens in vivo has not been shown. After BLV infection, however, production of antibodies directed towards BLV proteins (e.g. gp51) can be easily demonstrated. Thus, production of BLV proteins has to take place somewhere in infected cattle. Tissues and organs of experimentally infected cattle were fixed in acetone and embedded in paraffin. Monoclonal antibodies directed to gp51 were used to demonstrate BLV expression immunohistologically by the peroxidase-antiperoxidase (PAP) method. The same samples were also used to demonstrate a tumor associated antigen (TAA) employing a monoclonal antibody. Our results indicate that very few cells, found in the intestinal mucosa, produce gp51 in vivo. The expression of TAA, however, increases significantly shortly after infection with BLV and remains high throughout life.
UR - http://www.scopus.com/inward/record.url?scp=0024852279&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024852279&partnerID=8YFLogxK
U2 - 10.1016/0165-2427(89)90009-3
DO - 10.1016/0165-2427(89)90009-3
M3 - Article
C2 - 2560854
AN - SCOPUS:0024852279
VL - 22
SP - 223
EP - 231
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
SN - 0165-2427
IS - 3
ER -