Abstract
Human basal epithelium (myoepithelium)-specific (312C8–1) and luminal epithelium-specific (13H5) cytokeratin antibodies were applied to frozen sections of normal canine mammary tissues (seven), benign adenomas and hyperplasias (five), mixed tumors (12), and adenocarcinomas (18) to determine if epithelial subsets could be discriminated by the use of an avidin biotin peroxidase complex immunohistochemical procedure. The 312C8–1 and 13H5 antibodies were consistently reactive with basal and luminal epithelium, respectively, in the normal mammary gland (7/7) and in benign adenomas and hyperplasias (5/5). Mixed mammary tumors had similar basal and luminal epithelial reactivity and also had proliferating spindle-shaped stromal cells that were reactive with 312C8–1 (10/12) and 13H5 (4/12). The adenocarcinomas were subclassified into basal, luminal, and basal/luminal on the basis of 312C8–1 reactivity (4/18), 13H5 reactivity (2/18), and dual reactivity with mutually exclusive anatomic distribution (11/18), respectively. Those tumors with dual immunoreactivity were indicative of noninvasive carcinomas. Dogs with neoplasms that were reactive with 312C8–1 and nonreactive with 13H5 had local recurrence or distant metastasis within 2 weeks to 6 months after diagnosis. Other antibodies used for comparison were pan cytokeratin AE1/AE3, actin HHF35, and vimentin. 312C8–1 and 13H5 antibodies are specific for canine mammary basal and luminal epithelium, respectively, and by employing these antibodies, the origin and differentiation of canine mammary neoplasms can be determined more accurately than on the basis of hematoxylin and eosin-stained tissue alone.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 155-161 |
| Number of pages | 7 |
| Journal | Veterinary Pathology |
| Volume | 30 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1993 |
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Keywords
- Cytokeratins
- dogs
- immunohistochemistry
- mammary gland
- neoplasia
ASJC Scopus subject areas
- veterinary(all)
Cite this
Immunohistochemical Reactivity of Basal and Luminal Epithelium-specific Cytokeratin Antibodies within Normal and Neoplastic Canine Mammary Glands. / Griffey, Stephen M; Madewell, B. R.; Dairkee, S. H.; Hunt, J. E.; Naydan, D. K.; Higgins, Robert.
In: Veterinary Pathology, Vol. 30, No. 2, 1993, p. 155-161.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunohistochemical Reactivity of Basal and Luminal Epithelium-specific Cytokeratin Antibodies within Normal and Neoplastic Canine Mammary Glands
AU - Griffey, Stephen M
AU - Madewell, B. R.
AU - Dairkee, S. H.
AU - Hunt, J. E.
AU - Naydan, D. K.
AU - Higgins, Robert
PY - 1993
Y1 - 1993
N2 - Human basal epithelium (myoepithelium)-specific (312C8–1) and luminal epithelium-specific (13H5) cytokeratin antibodies were applied to frozen sections of normal canine mammary tissues (seven), benign adenomas and hyperplasias (five), mixed tumors (12), and adenocarcinomas (18) to determine if epithelial subsets could be discriminated by the use of an avidin biotin peroxidase complex immunohistochemical procedure. The 312C8–1 and 13H5 antibodies were consistently reactive with basal and luminal epithelium, respectively, in the normal mammary gland (7/7) and in benign adenomas and hyperplasias (5/5). Mixed mammary tumors had similar basal and luminal epithelial reactivity and also had proliferating spindle-shaped stromal cells that were reactive with 312C8–1 (10/12) and 13H5 (4/12). The adenocarcinomas were subclassified into basal, luminal, and basal/luminal on the basis of 312C8–1 reactivity (4/18), 13H5 reactivity (2/18), and dual reactivity with mutually exclusive anatomic distribution (11/18), respectively. Those tumors with dual immunoreactivity were indicative of noninvasive carcinomas. Dogs with neoplasms that were reactive with 312C8–1 and nonreactive with 13H5 had local recurrence or distant metastasis within 2 weeks to 6 months after diagnosis. Other antibodies used for comparison were pan cytokeratin AE1/AE3, actin HHF35, and vimentin. 312C8–1 and 13H5 antibodies are specific for canine mammary basal and luminal epithelium, respectively, and by employing these antibodies, the origin and differentiation of canine mammary neoplasms can be determined more accurately than on the basis of hematoxylin and eosin-stained tissue alone.
AB - Human basal epithelium (myoepithelium)-specific (312C8–1) and luminal epithelium-specific (13H5) cytokeratin antibodies were applied to frozen sections of normal canine mammary tissues (seven), benign adenomas and hyperplasias (five), mixed tumors (12), and adenocarcinomas (18) to determine if epithelial subsets could be discriminated by the use of an avidin biotin peroxidase complex immunohistochemical procedure. The 312C8–1 and 13H5 antibodies were consistently reactive with basal and luminal epithelium, respectively, in the normal mammary gland (7/7) and in benign adenomas and hyperplasias (5/5). Mixed mammary tumors had similar basal and luminal epithelial reactivity and also had proliferating spindle-shaped stromal cells that were reactive with 312C8–1 (10/12) and 13H5 (4/12). The adenocarcinomas were subclassified into basal, luminal, and basal/luminal on the basis of 312C8–1 reactivity (4/18), 13H5 reactivity (2/18), and dual reactivity with mutually exclusive anatomic distribution (11/18), respectively. Those tumors with dual immunoreactivity were indicative of noninvasive carcinomas. Dogs with neoplasms that were reactive with 312C8–1 and nonreactive with 13H5 had local recurrence or distant metastasis within 2 weeks to 6 months after diagnosis. Other antibodies used for comparison were pan cytokeratin AE1/AE3, actin HHF35, and vimentin. 312C8–1 and 13H5 antibodies are specific for canine mammary basal and luminal epithelium, respectively, and by employing these antibodies, the origin and differentiation of canine mammary neoplasms can be determined more accurately than on the basis of hematoxylin and eosin-stained tissue alone.
KW - Cytokeratins
KW - dogs
KW - immunohistochemistry
KW - mammary gland
KW - neoplasia
UR - http://www.scopus.com/inward/record.url?scp=0027568354&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027568354&partnerID=8YFLogxK
U2 - 10.1177/030098589303000208
DO - 10.1177/030098589303000208
M3 - Article
C2 - 7682368
AN - SCOPUS:0027568354
VL - 30
SP - 155
EP - 161
JO - Veterinary Pathology
JF - Veterinary Pathology
SN - 0300-9858
IS - 2
ER -