Immunohistochemical localization of a tenascin-like extracellular matrix protein in sea urchin embryos

John A. Anstrom, Eleanor J. Mackie, Richard P Tucker

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

We have used polyclonal antisera raised against vertebrate tenascin to identify and localize tenascin-like proteins in the developing sea urchin. These antisera recognize high-molecular weight proteins on immunoblots of sea urchin embryo homogenates that are similar in size and appearance to tenascin from vertebrates. These proteins appear as a doublet with an apparent molecular weight of 150 kDa and a larger, broad band with an apparent molecular weight of 350 kDa. Whole mounts of sea urchin embryos and larvae were stained with one of these antisera. The anti-tenascin stained the surface of primary mesenchyme cells during their phase of active migration. This staining was sensitive to detergent, suggesting that the protein recognized by the antiserum was associated with the cell surface. During later stages of development, the bulk of the antitenascin staining was found dispersed throughout the blastocoel matrix, and was no longer sensitive to detergent. We conclude that sea urchins express tenascin-like proteins during early stages of development, and that these proteins may play a role associated with primary mesenchyme cell morphogenesis.

Original languageEnglish (US)
Pages (from-to)169-173
Number of pages5
JournalRoux's Archives of Developmental Biology
Volume199
Issue number3
DOIs
StatePublished - Nov 1990
Externally publishedYes

Keywords

  • Cell motility
  • Embryogenesis
  • Mesenchyme
  • Tenascin

ASJC Scopus subject areas

  • Developmental Biology

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