Immunohistochemical identification of Campylobacter fetus in natural cases of bovine and ovine abortions

C. M. Campero, Mark L Anderson, R. L. Walker, Patricia C Blanchard, L. Barbano, P. Chiu, A. Martínez, G. Combessies, J. C. Bardon, J. Cordeviola

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

An immunohistochemistry (IHC) procedure for the detection of Campylobacter fetus antigens using an avidin-biotin complex technique was performed on formalin fixed bovine and ovine fetal tissues from 26 natural cases of Campylobacter spp. abortion (four ovine and 22 bovine). The species of Campylobacter isolated included C. fetus ssp. venerealis from 13 bovine fetuses, C. fetus ssp. fetus from two ovine and one bovine fetus, Campylobacter jejuni from seven bovine fetuses, Campylobacter lari from two ovine fetuses and an unspeciated Campylobacter species in one bovine fetus. Histologic lesions identified in the aborted fetuses included placentitis, serositis, pneumonia, gastroenteritis, hepatitis and encephalitis. Campylobacter fetus antigens were identified by IHC in 13 of 13 bovine fetuses from which C. fetus ssp. venerealis was isolated and in two of two ovine fetuses from which C. fetus ssp. fetus was isolated. The IHC stains were negative in tissues from seven bovine fetuses from which C. jejuni was isolated, one bovine fetus infected with C. fetus ssp. fetus, one bovine fetus infected with the unspeciated Campylobacter and two ovine fetuses infected with C. lari. In positive cases, the IHC stain most frequently identified bacteria in the lung and gastrointestinal tract. The C. fetus IHC procedure performed on formalin fixed tissues is a practical tool for the diagnosis of natural cases of ovine and bovine abortion caused by C. fetus.

Original languageEnglish (US)
Pages (from-to)138-141
Number of pages4
JournalJournal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health
Volume52
Issue number3
DOIs
StatePublished - Apr 2005

Fingerprint

Campylobacter fetus
abortion (animals)
fetus
Sheep
Fetus
sheep
cattle
immunohistochemistry
Campylobacter
Immunohistochemistry
Campylobacter lari
Campylobacter jejuni
formalin
Formaldehyde
Campylobacter fetus subsp. fetus
Coloring Agents
Serositis
Aborted Fetus
antigens
Antigens

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)
  • Microbiology

Cite this

Immunohistochemical identification of Campylobacter fetus in natural cases of bovine and ovine abortions. / Campero, C. M.; Anderson, Mark L; Walker, R. L.; Blanchard, Patricia C; Barbano, L.; Chiu, P.; Martínez, A.; Combessies, G.; Bardon, J. C.; Cordeviola, J.

In: Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health, Vol. 52, No. 3, 04.2005, p. 138-141.

Research output: Contribution to journalArticle

Campero, C. M. ; Anderson, Mark L ; Walker, R. L. ; Blanchard, Patricia C ; Barbano, L. ; Chiu, P. ; Martínez, A. ; Combessies, G. ; Bardon, J. C. ; Cordeviola, J. / Immunohistochemical identification of Campylobacter fetus in natural cases of bovine and ovine abortions. In: Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health. 2005 ; Vol. 52, No. 3. pp. 138-141.
@article{768a71afe87b41519609d863498d3275,
title = "Immunohistochemical identification of Campylobacter fetus in natural cases of bovine and ovine abortions",
abstract = "An immunohistochemistry (IHC) procedure for the detection of Campylobacter fetus antigens using an avidin-biotin complex technique was performed on formalin fixed bovine and ovine fetal tissues from 26 natural cases of Campylobacter spp. abortion (four ovine and 22 bovine). The species of Campylobacter isolated included C. fetus ssp. venerealis from 13 bovine fetuses, C. fetus ssp. fetus from two ovine and one bovine fetus, Campylobacter jejuni from seven bovine fetuses, Campylobacter lari from two ovine fetuses and an unspeciated Campylobacter species in one bovine fetus. Histologic lesions identified in the aborted fetuses included placentitis, serositis, pneumonia, gastroenteritis, hepatitis and encephalitis. Campylobacter fetus antigens were identified by IHC in 13 of 13 bovine fetuses from which C. fetus ssp. venerealis was isolated and in two of two ovine fetuses from which C. fetus ssp. fetus was isolated. The IHC stains were negative in tissues from seven bovine fetuses from which C. jejuni was isolated, one bovine fetus infected with C. fetus ssp. fetus, one bovine fetus infected with the unspeciated Campylobacter and two ovine fetuses infected with C. lari. In positive cases, the IHC stain most frequently identified bacteria in the lung and gastrointestinal tract. The C. fetus IHC procedure performed on formalin fixed tissues is a practical tool for the diagnosis of natural cases of ovine and bovine abortion caused by C. fetus.",
author = "Campero, {C. M.} and Anderson, {Mark L} and Walker, {R. L.} and Blanchard, {Patricia C} and L. Barbano and P. Chiu and A. Mart{\'i}nez and G. Combessies and Bardon, {J. C.} and J. Cordeviola",
year = "2005",
month = "4",
doi = "10.1111/j.1439-0450.2005.00834.x",
language = "English (US)",
volume = "52",
pages = "138--141",
journal = "Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health",
issn = "1863-1959",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Immunohistochemical identification of Campylobacter fetus in natural cases of bovine and ovine abortions

AU - Campero, C. M.

AU - Anderson, Mark L

AU - Walker, R. L.

AU - Blanchard, Patricia C

AU - Barbano, L.

AU - Chiu, P.

AU - Martínez, A.

AU - Combessies, G.

AU - Bardon, J. C.

AU - Cordeviola, J.

PY - 2005/4

Y1 - 2005/4

N2 - An immunohistochemistry (IHC) procedure for the detection of Campylobacter fetus antigens using an avidin-biotin complex technique was performed on formalin fixed bovine and ovine fetal tissues from 26 natural cases of Campylobacter spp. abortion (four ovine and 22 bovine). The species of Campylobacter isolated included C. fetus ssp. venerealis from 13 bovine fetuses, C. fetus ssp. fetus from two ovine and one bovine fetus, Campylobacter jejuni from seven bovine fetuses, Campylobacter lari from two ovine fetuses and an unspeciated Campylobacter species in one bovine fetus. Histologic lesions identified in the aborted fetuses included placentitis, serositis, pneumonia, gastroenteritis, hepatitis and encephalitis. Campylobacter fetus antigens were identified by IHC in 13 of 13 bovine fetuses from which C. fetus ssp. venerealis was isolated and in two of two ovine fetuses from which C. fetus ssp. fetus was isolated. The IHC stains were negative in tissues from seven bovine fetuses from which C. jejuni was isolated, one bovine fetus infected with C. fetus ssp. fetus, one bovine fetus infected with the unspeciated Campylobacter and two ovine fetuses infected with C. lari. In positive cases, the IHC stain most frequently identified bacteria in the lung and gastrointestinal tract. The C. fetus IHC procedure performed on formalin fixed tissues is a practical tool for the diagnosis of natural cases of ovine and bovine abortion caused by C. fetus.

AB - An immunohistochemistry (IHC) procedure for the detection of Campylobacter fetus antigens using an avidin-biotin complex technique was performed on formalin fixed bovine and ovine fetal tissues from 26 natural cases of Campylobacter spp. abortion (four ovine and 22 bovine). The species of Campylobacter isolated included C. fetus ssp. venerealis from 13 bovine fetuses, C. fetus ssp. fetus from two ovine and one bovine fetus, Campylobacter jejuni from seven bovine fetuses, Campylobacter lari from two ovine fetuses and an unspeciated Campylobacter species in one bovine fetus. Histologic lesions identified in the aborted fetuses included placentitis, serositis, pneumonia, gastroenteritis, hepatitis and encephalitis. Campylobacter fetus antigens were identified by IHC in 13 of 13 bovine fetuses from which C. fetus ssp. venerealis was isolated and in two of two ovine fetuses from which C. fetus ssp. fetus was isolated. The IHC stains were negative in tissues from seven bovine fetuses from which C. jejuni was isolated, one bovine fetus infected with C. fetus ssp. fetus, one bovine fetus infected with the unspeciated Campylobacter and two ovine fetuses infected with C. lari. In positive cases, the IHC stain most frequently identified bacteria in the lung and gastrointestinal tract. The C. fetus IHC procedure performed on formalin fixed tissues is a practical tool for the diagnosis of natural cases of ovine and bovine abortion caused by C. fetus.

UR - http://www.scopus.com/inward/record.url?scp=21044452011&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21044452011&partnerID=8YFLogxK

U2 - 10.1111/j.1439-0450.2005.00834.x

DO - 10.1111/j.1439-0450.2005.00834.x

M3 - Article

VL - 52

SP - 138

EP - 141

JO - Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health

JF - Journal of Veterinary Medicine Series B: Infectious Diseases and Veterinary Public Health

SN - 1863-1959

IS - 3

ER -