Abstract
Blackleg is caused by Clostridium chauvoei, whereas malignant oedema is caused by C. chauvoei, C. septicum, C. sordellii, C. perfringens type A, and/or C. novyi type A. Anti-C. chauvoei, anti-C. septicum, anti-C. sordellii and anti-C. novyi type A polyclonal antibodies were produced in rabbits and purified in a column of DEAE-cellulose. Aliquots of the antisera were conjugated with fluorescein isothiocyanate and the remaining was used for the streptavidin biotin peroxidase technique (SBPT). SBPT was standardized to detect C. chauvoei, C. septicum, C. sordellii and C. novyi type A in formalin-fixed, paraffin-embedded tissues of guinea pigs. SBPT was compared to a fluorescent antibody technique (FAT). Sections and smears of muscle from inoculation area (MIA), heart, liver, spleen and kidney, were obtained for both SBPT and FAT. Cross-reactions between the different Clostridial species were not observed. C. chauvoei and C. septicum were detected in all specimens from the animals inoculated with these microorganisms, while only sections of muscle obtained from all the animals inoculated with C. sordellii and C. novyi type A were positive. The same results observed by the SBPT, were obtained on tissue smears of these microorganisms stained by the FAT. The results indicate that SBPT is suitable for detection of C. chauvoei, C. septicum, C. sordellii and C. novyi type A in formalin-fixed, paraffin-embedded tissues of guinea pigs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4-8 |
| Number of pages | 5 |
| Journal | Pesquisa Veterinaria Brasileira |
| Volume | 25 |
| Issue number | 1 |
| State | Published - Jan 2005 |
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Keywords
- Blackleg
- Clostridia
- Fluorescent antibody technique
- Guinea pigs
- Malignant oedema
- Streptavidin biotin peroxidase technique
ASJC Scopus subject areas
- veterinary(all)
Cite this
Immunohistochemical detection of Clostridia species in paraffin-embedded tissues of experimentally inoculated guinea pigs. / Assis, Ronnie A.; Lobato, Francisco C F; Serakides, Rogéria; Santos, Renato L.; Dias, Guilherme R C; Nascimento, Ricardo A P; Abreu, Vera L V; Parreiras, Patrícia M.; Uzal, Francisco A.
In: Pesquisa Veterinaria Brasileira, Vol. 25, No. 1, 01.2005, p. 4-8.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunohistochemical detection of Clostridia species in paraffin-embedded tissues of experimentally inoculated guinea pigs
AU - Assis, Ronnie A.
AU - Lobato, Francisco C F
AU - Serakides, Rogéria
AU - Santos, Renato L.
AU - Dias, Guilherme R C
AU - Nascimento, Ricardo A P
AU - Abreu, Vera L V
AU - Parreiras, Patrícia M.
AU - Uzal, Francisco A
PY - 2005/1
Y1 - 2005/1
N2 - Blackleg is caused by Clostridium chauvoei, whereas malignant oedema is caused by C. chauvoei, C. septicum, C. sordellii, C. perfringens type A, and/or C. novyi type A. Anti-C. chauvoei, anti-C. septicum, anti-C. sordellii and anti-C. novyi type A polyclonal antibodies were produced in rabbits and purified in a column of DEAE-cellulose. Aliquots of the antisera were conjugated with fluorescein isothiocyanate and the remaining was used for the streptavidin biotin peroxidase technique (SBPT). SBPT was standardized to detect C. chauvoei, C. septicum, C. sordellii and C. novyi type A in formalin-fixed, paraffin-embedded tissues of guinea pigs. SBPT was compared to a fluorescent antibody technique (FAT). Sections and smears of muscle from inoculation area (MIA), heart, liver, spleen and kidney, were obtained for both SBPT and FAT. Cross-reactions between the different Clostridial species were not observed. C. chauvoei and C. septicum were detected in all specimens from the animals inoculated with these microorganisms, while only sections of muscle obtained from all the animals inoculated with C. sordellii and C. novyi type A were positive. The same results observed by the SBPT, were obtained on tissue smears of these microorganisms stained by the FAT. The results indicate that SBPT is suitable for detection of C. chauvoei, C. septicum, C. sordellii and C. novyi type A in formalin-fixed, paraffin-embedded tissues of guinea pigs.
AB - Blackleg is caused by Clostridium chauvoei, whereas malignant oedema is caused by C. chauvoei, C. septicum, C. sordellii, C. perfringens type A, and/or C. novyi type A. Anti-C. chauvoei, anti-C. septicum, anti-C. sordellii and anti-C. novyi type A polyclonal antibodies were produced in rabbits and purified in a column of DEAE-cellulose. Aliquots of the antisera were conjugated with fluorescein isothiocyanate and the remaining was used for the streptavidin biotin peroxidase technique (SBPT). SBPT was standardized to detect C. chauvoei, C. septicum, C. sordellii and C. novyi type A in formalin-fixed, paraffin-embedded tissues of guinea pigs. SBPT was compared to a fluorescent antibody technique (FAT). Sections and smears of muscle from inoculation area (MIA), heart, liver, spleen and kidney, were obtained for both SBPT and FAT. Cross-reactions between the different Clostridial species were not observed. C. chauvoei and C. septicum were detected in all specimens from the animals inoculated with these microorganisms, while only sections of muscle obtained from all the animals inoculated with C. sordellii and C. novyi type A were positive. The same results observed by the SBPT, were obtained on tissue smears of these microorganisms stained by the FAT. The results indicate that SBPT is suitable for detection of C. chauvoei, C. septicum, C. sordellii and C. novyi type A in formalin-fixed, paraffin-embedded tissues of guinea pigs.
KW - Blackleg
KW - Clostridia
KW - Fluorescent antibody technique
KW - Guinea pigs
KW - Malignant oedema
KW - Streptavidin biotin peroxidase technique
UR - http://www.scopus.com/inward/record.url?scp=20044391824&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=20044391824&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:20044391824
VL - 25
SP - 4
EP - 8
JO - Pesquisa Veterinaria Brasileira
JF - Pesquisa Veterinaria Brasileira
SN - 0100-736X
IS - 1
ER -