Immunohistochemical demonstration of cytochrome p-450 monooxygenase in clara cells throughout the tracheobronchial airways of the rabbit

Charles Plopper, D. L. Cranz, L. Kemp, C. J. Serabjit-Singh, R. M. Philpot

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The nonciliated bronchiolar epithelial (or Clara) cell is considered the primary pulmonary site of cytochrome P-450-dependent monooxygenase activity. Despite the general conception that Clara cells are restricted to bronchioles, we have previously shown that a nonciliated cell with the cytological features of the Clara cell predominates throughout rabbit tracheobronchial airways. The present study was designed to determine if the cytochrome P-450 monooxygenase system has the same distribution. Trachea, terminal bronchioles and 3 generations of bronchi were selected by microdissection from fixed lungs of adult specific-pathogen-free rabbits. Serial sections of paraffin-embedded tissue were stained with either Alcian blue/periodic acid Schijfs (AB/PAS) or with antisera to one of the following: cytochrome P-450 form 2, form 5, or NADPH-dependent cytochrome P-450 reductase. The majority of nonciliated epithelial cells lining all 5 airway generations were PAS+ and AB-. Nonciliated cells in all 5 airway generations reacted positively with all three antisera. The primary deposition site was the apical portion of nonciliated cells. Other sites included ciliated surfaces and vascular endothelium. Reaction products from all three antisera had the same localization pattern. We conclude that (1) the cytochrome P-450 monooxygenase system is distributed throughout the tracheobronchial airways of the rabbit and (2) the Clara cells of the trachea and bronchi are functionally, as well as structurally, similar to those of the bronchioles.

Original languageEnglish (US)
Pages (from-to)59-68
Number of pages10
JournalExperimental Lung Research
Volume13
Issue number1
DOIs
StatePublished - Jan 1 1987

Fingerprint

Cytochromes
Mixed Function Oxygenases
Cytochrome P-450 Enzyme System
Demonstrations
Rabbits
Immune Sera
Bronchioles
Microdissection
Alcian Blue
NADPH-Ferrihemoprotein Reductase
Periodic Acid
Bronchi
Trachea
Pathogens
NADP
Reaction products
Linings
Paraffin
Specific Pathogen-Free Organisms
Computer Communication Networks

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry

Cite this

Immunohistochemical demonstration of cytochrome p-450 monooxygenase in clara cells throughout the tracheobronchial airways of the rabbit. / Plopper, Charles; Cranz, D. L.; Kemp, L.; Serabjit-Singh, C. J.; Philpot, R. M.

In: Experimental Lung Research, Vol. 13, No. 1, 01.01.1987, p. 59-68.

Research output: Contribution to journalArticle

@article{8c0e403646c94966acc951037a08cfce,
title = "Immunohistochemical demonstration of cytochrome p-450 monooxygenase in clara cells throughout the tracheobronchial airways of the rabbit",
abstract = "The nonciliated bronchiolar epithelial (or Clara) cell is considered the primary pulmonary site of cytochrome P-450-dependent monooxygenase activity. Despite the general conception that Clara cells are restricted to bronchioles, we have previously shown that a nonciliated cell with the cytological features of the Clara cell predominates throughout rabbit tracheobronchial airways. The present study was designed to determine if the cytochrome P-450 monooxygenase system has the same distribution. Trachea, terminal bronchioles and 3 generations of bronchi were selected by microdissection from fixed lungs of adult specific-pathogen-free rabbits. Serial sections of paraffin-embedded tissue were stained with either Alcian blue/periodic acid Schijfs (AB/PAS) or with antisera to one of the following: cytochrome P-450 form 2, form 5, or NADPH-dependent cytochrome P-450 reductase. The majority of nonciliated epithelial cells lining all 5 airway generations were PAS+ and AB-. Nonciliated cells in all 5 airway generations reacted positively with all three antisera. The primary deposition site was the apical portion of nonciliated cells. Other sites included ciliated surfaces and vascular endothelium. Reaction products from all three antisera had the same localization pattern. We conclude that (1) the cytochrome P-450 monooxygenase system is distributed throughout the tracheobronchial airways of the rabbit and (2) the Clara cells of the trachea and bronchi are functionally, as well as structurally, similar to those of the bronchioles.",
author = "Charles Plopper and Cranz, {D. L.} and L. Kemp and Serabjit-Singh, {C. J.} and Philpot, {R. M.}",
year = "1987",
month = "1",
day = "1",
doi = "10.3109/01902148709064309",
language = "English (US)",
volume = "13",
pages = "59--68",
journal = "Experimental Lung Research",
issn = "0190-2148",
publisher = "Informa Healthcare",
number = "1",

}

TY - JOUR

T1 - Immunohistochemical demonstration of cytochrome p-450 monooxygenase in clara cells throughout the tracheobronchial airways of the rabbit

AU - Plopper, Charles

AU - Cranz, D. L.

AU - Kemp, L.

AU - Serabjit-Singh, C. J.

AU - Philpot, R. M.

PY - 1987/1/1

Y1 - 1987/1/1

N2 - The nonciliated bronchiolar epithelial (or Clara) cell is considered the primary pulmonary site of cytochrome P-450-dependent monooxygenase activity. Despite the general conception that Clara cells are restricted to bronchioles, we have previously shown that a nonciliated cell with the cytological features of the Clara cell predominates throughout rabbit tracheobronchial airways. The present study was designed to determine if the cytochrome P-450 monooxygenase system has the same distribution. Trachea, terminal bronchioles and 3 generations of bronchi were selected by microdissection from fixed lungs of adult specific-pathogen-free rabbits. Serial sections of paraffin-embedded tissue were stained with either Alcian blue/periodic acid Schijfs (AB/PAS) or with antisera to one of the following: cytochrome P-450 form 2, form 5, or NADPH-dependent cytochrome P-450 reductase. The majority of nonciliated epithelial cells lining all 5 airway generations were PAS+ and AB-. Nonciliated cells in all 5 airway generations reacted positively with all three antisera. The primary deposition site was the apical portion of nonciliated cells. Other sites included ciliated surfaces and vascular endothelium. Reaction products from all three antisera had the same localization pattern. We conclude that (1) the cytochrome P-450 monooxygenase system is distributed throughout the tracheobronchial airways of the rabbit and (2) the Clara cells of the trachea and bronchi are functionally, as well as structurally, similar to those of the bronchioles.

AB - The nonciliated bronchiolar epithelial (or Clara) cell is considered the primary pulmonary site of cytochrome P-450-dependent monooxygenase activity. Despite the general conception that Clara cells are restricted to bronchioles, we have previously shown that a nonciliated cell with the cytological features of the Clara cell predominates throughout rabbit tracheobronchial airways. The present study was designed to determine if the cytochrome P-450 monooxygenase system has the same distribution. Trachea, terminal bronchioles and 3 generations of bronchi were selected by microdissection from fixed lungs of adult specific-pathogen-free rabbits. Serial sections of paraffin-embedded tissue were stained with either Alcian blue/periodic acid Schijfs (AB/PAS) or with antisera to one of the following: cytochrome P-450 form 2, form 5, or NADPH-dependent cytochrome P-450 reductase. The majority of nonciliated epithelial cells lining all 5 airway generations were PAS+ and AB-. Nonciliated cells in all 5 airway generations reacted positively with all three antisera. The primary deposition site was the apical portion of nonciliated cells. Other sites included ciliated surfaces and vascular endothelium. Reaction products from all three antisera had the same localization pattern. We conclude that (1) the cytochrome P-450 monooxygenase system is distributed throughout the tracheobronchial airways of the rabbit and (2) the Clara cells of the trachea and bronchi are functionally, as well as structurally, similar to those of the bronchioles.

UR - http://www.scopus.com/inward/record.url?scp=0023080998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023080998&partnerID=8YFLogxK

U2 - 10.3109/01902148709064309

DO - 10.3109/01902148709064309

M3 - Article

VL - 13

SP - 59

EP - 68

JO - Experimental Lung Research

JF - Experimental Lung Research

SN - 0190-2148

IS - 1

ER -