Immunohistochemical Characteristics of Normal Canine Eyes

Philippe Labelle, C. M. Reilly, D. K. Naydan, A. L. Labelle

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Immunohistochemistry is widely utilized in diagnostic laboratories to study neoplastic and nonneoplastic diseases. Knowledge of the immunohistochemical characteristics of normal tissue is essential for interpretation of immunoreactivity in pathologic conditions. In this study, immunohistochemistry was performed with a broad panel of diagnostically relevant antibodies on 4 normal canine globes-namely, vimentin, pan-cytokeratin (AE1/AE3), cytokeratin 7, cytokeratin 8/18, cytokeratin 20, α-smooth muscle actin, muscle specific actin, desmin, Melan-A, microphthalmia transcription factor, S-100, glial fibrillary acidic protein, triple neurofilaments, neuron-specific enolase, chromogranin A, synaptophysin, laminin and CD31. Results include cytokeratin immunoreactivity limited to the conjunctival epithelium, corneal epithelium, and retinal pigment epithelium; distinct patterns of immunopositivity of muscle markers; and widespread immunoreactivity for vimentin and most neural/neuroendocrine markers. These findings in normal eyes provide the basis for interpretation of ocular immunohistochemistry in dogs. Published immunophenotypes of primary ocular neoplasms are also reviewed.

Original languageEnglish (US)
Pages (from-to)860-869
Number of pages10
JournalVeterinary Pathology
Volume49
Issue number5
DOIs
StatePublished - Sep 2012

Fingerprint

immunohistochemistry
Canidae
epithelium
eyes
vimentin
Immunohistochemistry
Vimentin
Keratins
actin
Actins
dogs
Eye Neoplasms
Microphthalmia-Associated Transcription Factor
MART-1 Antigen
Keratin-20
Keratin-8
Keratin-7
Keratin-18
Chromogranin A
Muscles

Keywords

  • dogs
  • eye
  • immunohistochemistry
  • neoplasms
  • review

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Labelle, P., Reilly, C. M., Naydan, D. K., & Labelle, A. L. (2012). Immunohistochemical Characteristics of Normal Canine Eyes. Veterinary Pathology, 49(5), 860-869. https://doi.org/10.1177/0300985811427152

Immunohistochemical Characteristics of Normal Canine Eyes. / Labelle, Philippe; Reilly, C. M.; Naydan, D. K.; Labelle, A. L.

In: Veterinary Pathology, Vol. 49, No. 5, 09.2012, p. 860-869.

Research output: Contribution to journalArticle

Labelle, P, Reilly, CM, Naydan, DK & Labelle, AL 2012, 'Immunohistochemical Characteristics of Normal Canine Eyes', Veterinary Pathology, vol. 49, no. 5, pp. 860-869. https://doi.org/10.1177/0300985811427152
Labelle, Philippe ; Reilly, C. M. ; Naydan, D. K. ; Labelle, A. L. / Immunohistochemical Characteristics of Normal Canine Eyes. In: Veterinary Pathology. 2012 ; Vol. 49, No. 5. pp. 860-869.
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