Immunohistochemical Characteristics of Normal Canine Eyes

Philippe Labelle, C. M. Reilly, D. K. Naydan, A. L. Labelle

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Immunohistochemistry is widely utilized in diagnostic laboratories to study neoplastic and nonneoplastic diseases. Knowledge of the immunohistochemical characteristics of normal tissue is essential for interpretation of immunoreactivity in pathologic conditions. In this study, immunohistochemistry was performed with a broad panel of diagnostically relevant antibodies on 4 normal canine globes-namely, vimentin, pan-cytokeratin (AE1/AE3), cytokeratin 7, cytokeratin 8/18, cytokeratin 20, α-smooth muscle actin, muscle specific actin, desmin, Melan-A, microphthalmia transcription factor, S-100, glial fibrillary acidic protein, triple neurofilaments, neuron-specific enolase, chromogranin A, synaptophysin, laminin and CD31. Results include cytokeratin immunoreactivity limited to the conjunctival epithelium, corneal epithelium, and retinal pigment epithelium; distinct patterns of immunopositivity of muscle markers; and widespread immunoreactivity for vimentin and most neural/neuroendocrine markers. These findings in normal eyes provide the basis for interpretation of ocular immunohistochemistry in dogs. Published immunophenotypes of primary ocular neoplasms are also reviewed.

Original languageEnglish (US)
Pages (from-to)860-869
Number of pages10
JournalVeterinary Pathology
Volume49
Issue number5
DOIs
StatePublished - Sep 2012

Keywords

  • dogs
  • eye
  • immunohistochemistry
  • neoplasms
  • review

ASJC Scopus subject areas

  • veterinary(all)

Fingerprint Dive into the research topics of 'Immunohistochemical Characteristics of Normal Canine Eyes'. Together they form a unique fingerprint.

  • Cite this

    Labelle, P., Reilly, C. M., Naydan, D. K., & Labelle, A. L. (2012). Immunohistochemical Characteristics of Normal Canine Eyes. Veterinary Pathology, 49(5), 860-869. https://doi.org/10.1177/0300985811427152