Abstract
The role and diagnostic efficacy of gene and protein products RB1, CDK4, CHMP2B, HSP90, and cPLA2G4A, all previously shown to be involved in tumor genesis and cell proliferation, were examined by immunohistochemical techniques in 32 cases of myxofibrosarcomas and 29 myxoid liposarcomas (all diagnosis had been confirmed by fluorescence in situ hybridization). HSP90 demonstrated strong nuclear and cytoplasmic positivity in all myxoid liposarcoma cases, while only 4 myxofibrosarcomas showed scattered HSP90 positivity. All but 4 cases of myxofibrosarcoma displayed strong positivity for cPLA2G4A, while only 2 myxoid liposarcoma cases were cPLA2G4A positive and both were CHMP2B negative. Overexpression of both cPLA2G4A and CHMP2B also suggested higher tumor grade. In conclusion, HSP90 and cPLA2G4A immunohistochemical stains are useful markers to distinguish myxofibrosarcoma from myxoid liposarcoma.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 589-599 |
| Number of pages | 11 |
| Journal | International Journal of Surgical Pathology |
| Volume | 22 |
| Issue number | 7 |
| DOIs | |
| State | Published - Oct 1 2014 |
| Externally published | Yes |
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Keywords
- CDK4
- CHMP2B
- CPLA2G4A
- HSP90
- myxofibrosarcoma
- myxoid liposarcoma
- RB1
ASJC Scopus subject areas
- Anatomy
- Surgery
- Pathology and Forensic Medicine
Cite this
Immunohistochemical analysis of expressions of RB1, CDK4, HSP90, cPLA2G4A, and CHMP2B is helpful in distinction between myxofibrosarcoma and myxoid liposarcoma. / Wang, Tao; Goodman, Mark A.; McGough, Richard L.; Weiss, Kurt R.; Rao, Uma N.M.
In: International Journal of Surgical Pathology, Vol. 22, No. 7, 01.10.2014, p. 589-599.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunohistochemical analysis of expressions of RB1, CDK4, HSP90, cPLA2G4A, and CHMP2B is helpful in distinction between myxofibrosarcoma and myxoid liposarcoma
AU - Wang, Tao
AU - Goodman, Mark A.
AU - McGough, Richard L.
AU - Weiss, Kurt R.
AU - Rao, Uma N.M.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - The role and diagnostic efficacy of gene and protein products RB1, CDK4, CHMP2B, HSP90, and cPLA2G4A, all previously shown to be involved in tumor genesis and cell proliferation, were examined by immunohistochemical techniques in 32 cases of myxofibrosarcomas and 29 myxoid liposarcomas (all diagnosis had been confirmed by fluorescence in situ hybridization). HSP90 demonstrated strong nuclear and cytoplasmic positivity in all myxoid liposarcoma cases, while only 4 myxofibrosarcomas showed scattered HSP90 positivity. All but 4 cases of myxofibrosarcoma displayed strong positivity for cPLA2G4A, while only 2 myxoid liposarcoma cases were cPLA2G4A positive and both were CHMP2B negative. Overexpression of both cPLA2G4A and CHMP2B also suggested higher tumor grade. In conclusion, HSP90 and cPLA2G4A immunohistochemical stains are useful markers to distinguish myxofibrosarcoma from myxoid liposarcoma.
AB - The role and diagnostic efficacy of gene and protein products RB1, CDK4, CHMP2B, HSP90, and cPLA2G4A, all previously shown to be involved in tumor genesis and cell proliferation, were examined by immunohistochemical techniques in 32 cases of myxofibrosarcomas and 29 myxoid liposarcomas (all diagnosis had been confirmed by fluorescence in situ hybridization). HSP90 demonstrated strong nuclear and cytoplasmic positivity in all myxoid liposarcoma cases, while only 4 myxofibrosarcomas showed scattered HSP90 positivity. All but 4 cases of myxofibrosarcoma displayed strong positivity for cPLA2G4A, while only 2 myxoid liposarcoma cases were cPLA2G4A positive and both were CHMP2B negative. Overexpression of both cPLA2G4A and CHMP2B also suggested higher tumor grade. In conclusion, HSP90 and cPLA2G4A immunohistochemical stains are useful markers to distinguish myxofibrosarcoma from myxoid liposarcoma.
KW - CDK4
KW - CHMP2B
KW - CPLA2G4A
KW - HSP90
KW - myxofibrosarcoma
KW - myxoid liposarcoma
KW - RB1
UR - http://www.scopus.com/inward/record.url?scp=84907618479&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907618479&partnerID=8YFLogxK
U2 - 10.1177/1066896914532539
DO - 10.1177/1066896914532539
M3 - Article
VL - 22
SP - 589
EP - 599
JO - International Journal of Surgical Pathology
JF - International Journal of Surgical Pathology
SN - 1066-8969
IS - 7
ER -