Immunohistochemical analysis of cell-matrix adhesion molecules and their ligands in the portal tracts of primary biliary cirrhosis

Mitsue Yasoshima, Koichi Tsuneyama, Kenichi Harada, Motoko Sasaki, M. Eric Gershwin, Yasuni Nakanuma

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Intraepithelial migration of lymphoid cells (epitheliotropism) through the basement membrane of the bile duct is a key event in the development of chronic non-suppurative destructive cholangitis (CNSDC) and eventual immune- mediated bile duct loss in primary biliary cirrhosis (PBC). Cell-to-cell and cell-to-extracellular matrix proteins may play an important role in the development of CNSDC. To address the events of epitheliotropism in CNSDC, the expression of cell-matrix adhesion molecules such as integrins α1, α3, α4, α5, and α6 and the distribution of fibronectin, laminin, and collagen type IV were studied immunohistochemically, with emphasis on both infiltrating lymphocytes and the bile ducts, in frozen sections from 15 PBC cases and 34 controls (chronic viral hepatitis, extrahepatic biliary obstruction, and normal liver). In PBC and chronic viral hepatitis, most of the infiltrating lymphoid cells expressed integrin α4, while such expression was less common in extrahepatic biliary obstruction and normal liver. A biliary basement membrane-like structure was delineated by immunostaining of collagen type IV and laminin in PBC and controls. On the basement membrane of CNSDC, fibronectin, a ligand of integrin α4, was strongly and frequently expressed in PBC, while such expression on the biliary basement membrane was rare in other controls. These results suggest that increased fibronectin expression on the biliary basement membrane and integrin α4-fibronectin interaction facilitate adhesion and the penetration of infiltrating α4-expressing lymphocytes into the biliary epithelial layer in PBC.

Original languageEnglish (US)
Pages (from-to)93-99
Number of pages7
JournalJournal of Pathology
Volume190
Issue number1
DOIs
StatePublished - Jan 2000

Fingerprint

Cell-Matrix Junctions
Biliary Liver Cirrhosis
Cell Adhesion Molecules
Basement Membrane
Cholangitis
Ligands
Fibronectins
Integrins
Bile Ducts
Lymphocytes
Collagen Type IV
Laminin
Chronic Hepatitis
Extracellular Matrix Proteins
Liver
Frozen Sections

Keywords

  • Biliary tree
  • Epitheliotropism
  • Fibronectin
  • Integrin
  • Primary biliary cirrhosis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Immunohistochemical analysis of cell-matrix adhesion molecules and their ligands in the portal tracts of primary biliary cirrhosis. / Yasoshima, Mitsue; Tsuneyama, Koichi; Harada, Kenichi; Sasaki, Motoko; Gershwin, M. Eric; Nakanuma, Yasuni.

In: Journal of Pathology, Vol. 190, No. 1, 01.2000, p. 93-99.

Research output: Contribution to journalArticle

Yasoshima, Mitsue ; Tsuneyama, Koichi ; Harada, Kenichi ; Sasaki, Motoko ; Gershwin, M. Eric ; Nakanuma, Yasuni. / Immunohistochemical analysis of cell-matrix adhesion molecules and their ligands in the portal tracts of primary biliary cirrhosis. In: Journal of Pathology. 2000 ; Vol. 190, No. 1. pp. 93-99.
@article{fc531968273849e49d5e73f00b922d80,
title = "Immunohistochemical analysis of cell-matrix adhesion molecules and their ligands in the portal tracts of primary biliary cirrhosis",
abstract = "Intraepithelial migration of lymphoid cells (epitheliotropism) through the basement membrane of the bile duct is a key event in the development of chronic non-suppurative destructive cholangitis (CNSDC) and eventual immune- mediated bile duct loss in primary biliary cirrhosis (PBC). Cell-to-cell and cell-to-extracellular matrix proteins may play an important role in the development of CNSDC. To address the events of epitheliotropism in CNSDC, the expression of cell-matrix adhesion molecules such as integrins α1, α3, α4, α5, and α6 and the distribution of fibronectin, laminin, and collagen type IV were studied immunohistochemically, with emphasis on both infiltrating lymphocytes and the bile ducts, in frozen sections from 15 PBC cases and 34 controls (chronic viral hepatitis, extrahepatic biliary obstruction, and normal liver). In PBC and chronic viral hepatitis, most of the infiltrating lymphoid cells expressed integrin α4, while such expression was less common in extrahepatic biliary obstruction and normal liver. A biliary basement membrane-like structure was delineated by immunostaining of collagen type IV and laminin in PBC and controls. On the basement membrane of CNSDC, fibronectin, a ligand of integrin α4, was strongly and frequently expressed in PBC, while such expression on the biliary basement membrane was rare in other controls. These results suggest that increased fibronectin expression on the biliary basement membrane and integrin α4-fibronectin interaction facilitate adhesion and the penetration of infiltrating α4-expressing lymphocytes into the biliary epithelial layer in PBC.",
keywords = "Biliary tree, Epitheliotropism, Fibronectin, Integrin, Primary biliary cirrhosis",
author = "Mitsue Yasoshima and Koichi Tsuneyama and Kenichi Harada and Motoko Sasaki and Gershwin, {M. Eric} and Yasuni Nakanuma",
year = "2000",
month = "1",
doi = "10.1002/(SICI)1096-9896(200001)190:1<93::AID-PATH507>3.0.CO;2-A",
language = "English (US)",
volume = "190",
pages = "93--99",
journal = "Journal of Pathology",
issn = "0022-3417",
publisher = "John Wiley and Sons Ltd",
number = "1",

}

TY - JOUR

T1 - Immunohistochemical analysis of cell-matrix adhesion molecules and their ligands in the portal tracts of primary biliary cirrhosis

AU - Yasoshima, Mitsue

AU - Tsuneyama, Koichi

AU - Harada, Kenichi

AU - Sasaki, Motoko

AU - Gershwin, M. Eric

AU - Nakanuma, Yasuni

PY - 2000/1

Y1 - 2000/1

N2 - Intraepithelial migration of lymphoid cells (epitheliotropism) through the basement membrane of the bile duct is a key event in the development of chronic non-suppurative destructive cholangitis (CNSDC) and eventual immune- mediated bile duct loss in primary biliary cirrhosis (PBC). Cell-to-cell and cell-to-extracellular matrix proteins may play an important role in the development of CNSDC. To address the events of epitheliotropism in CNSDC, the expression of cell-matrix adhesion molecules such as integrins α1, α3, α4, α5, and α6 and the distribution of fibronectin, laminin, and collagen type IV were studied immunohistochemically, with emphasis on both infiltrating lymphocytes and the bile ducts, in frozen sections from 15 PBC cases and 34 controls (chronic viral hepatitis, extrahepatic biliary obstruction, and normal liver). In PBC and chronic viral hepatitis, most of the infiltrating lymphoid cells expressed integrin α4, while such expression was less common in extrahepatic biliary obstruction and normal liver. A biliary basement membrane-like structure was delineated by immunostaining of collagen type IV and laminin in PBC and controls. On the basement membrane of CNSDC, fibronectin, a ligand of integrin α4, was strongly and frequently expressed in PBC, while such expression on the biliary basement membrane was rare in other controls. These results suggest that increased fibronectin expression on the biliary basement membrane and integrin α4-fibronectin interaction facilitate adhesion and the penetration of infiltrating α4-expressing lymphocytes into the biliary epithelial layer in PBC.

AB - Intraepithelial migration of lymphoid cells (epitheliotropism) through the basement membrane of the bile duct is a key event in the development of chronic non-suppurative destructive cholangitis (CNSDC) and eventual immune- mediated bile duct loss in primary biliary cirrhosis (PBC). Cell-to-cell and cell-to-extracellular matrix proteins may play an important role in the development of CNSDC. To address the events of epitheliotropism in CNSDC, the expression of cell-matrix adhesion molecules such as integrins α1, α3, α4, α5, and α6 and the distribution of fibronectin, laminin, and collagen type IV were studied immunohistochemically, with emphasis on both infiltrating lymphocytes and the bile ducts, in frozen sections from 15 PBC cases and 34 controls (chronic viral hepatitis, extrahepatic biliary obstruction, and normal liver). In PBC and chronic viral hepatitis, most of the infiltrating lymphoid cells expressed integrin α4, while such expression was less common in extrahepatic biliary obstruction and normal liver. A biliary basement membrane-like structure was delineated by immunostaining of collagen type IV and laminin in PBC and controls. On the basement membrane of CNSDC, fibronectin, a ligand of integrin α4, was strongly and frequently expressed in PBC, while such expression on the biliary basement membrane was rare in other controls. These results suggest that increased fibronectin expression on the biliary basement membrane and integrin α4-fibronectin interaction facilitate adhesion and the penetration of infiltrating α4-expressing lymphocytes into the biliary epithelial layer in PBC.

KW - Biliary tree

KW - Epitheliotropism

KW - Fibronectin

KW - Integrin

KW - Primary biliary cirrhosis

UR - http://www.scopus.com/inward/record.url?scp=0033955026&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033955026&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1096-9896(200001)190:1<93::AID-PATH507>3.0.CO;2-A

DO - 10.1002/(SICI)1096-9896(200001)190:1<93::AID-PATH507>3.0.CO;2-A

M3 - Article

C2 - 10640998

AN - SCOPUS:0033955026

VL - 190

SP - 93

EP - 99

JO - Journal of Pathology

JF - Journal of Pathology

SN - 0022-3417

IS - 1

ER -