Immunohistochemical analysis of BCL-2 protein expression in renal cell carcinoma

Andrew Huang, Patricia D. Fone, Regina F Gandour-Edwards, Ralph W deVere White, Roger Low

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Purpose: To investigate the incidence, extent, and distribution of bcl-2 protein expression in human renal cell carcinomas. Materials and Methods: Using immunohistochemical tissue staining techniques, bcl-2 protein expression was analyzed in archival nephrectomy specimens removed for renal cell carcinoma or trauma and in 3 renal cell carcinoma cell lines. Results: Normal kidneys demonstrated bcl-2 immunopositivity primarily within the cytoplasm of distal tubule cells. Only rare and minor staining of the proximal tubular cells, thought to be the origin of renal cell carcinoma, was noted in histologically normal controls and areas adjacent to tumor. In contrast, bcl-2 protein expression was demonstrated in 70% of renal cell carcinomas and in all 3 experimental cell lines. Conclusions: Bcl-2 is a proto-oncogene known to regulate apoptosis (programmed cell death). Bcl-2 protein is overexpressed in the majority of renal cell carcinomas examined. Bcl-2 overexpression may have a role in tumorigenesis and may explain the relative resistance of renal cell carcinoma to chemotherapeutic agents and to radiation therapy.

Original languageEnglish (US)
Pages (from-to)610-613
Number of pages4
JournalJournal of Urology
Volume162
Issue number2
DOIs
StatePublished - Aug 1999

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Renal Cell Carcinoma
Proteins
Staining and Labeling
Cell Line
Proto-Oncogenes
Nephrectomy
Carcinogenesis
Cytoplasm
Cell Death
Radiotherapy
Apoptosis
Kidney
Incidence
Wounds and Injuries
Neoplasms

Keywords

  • Bcl-2
  • Immunohistochemistry
  • Renal cell carcinoma

ASJC Scopus subject areas

  • Urology

Cite this

Immunohistochemical analysis of BCL-2 protein expression in renal cell carcinoma. / Huang, Andrew; Fone, Patricia D.; Gandour-Edwards, Regina F; deVere White, Ralph W; Low, Roger.

In: Journal of Urology, Vol. 162, No. 2, 08.1999, p. 610-613.

Research output: Contribution to journalArticle

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abstract = "Purpose: To investigate the incidence, extent, and distribution of bcl-2 protein expression in human renal cell carcinomas. Materials and Methods: Using immunohistochemical tissue staining techniques, bcl-2 protein expression was analyzed in archival nephrectomy specimens removed for renal cell carcinoma or trauma and in 3 renal cell carcinoma cell lines. Results: Normal kidneys demonstrated bcl-2 immunopositivity primarily within the cytoplasm of distal tubule cells. Only rare and minor staining of the proximal tubular cells, thought to be the origin of renal cell carcinoma, was noted in histologically normal controls and areas adjacent to tumor. In contrast, bcl-2 protein expression was demonstrated in 70{\%} of renal cell carcinomas and in all 3 experimental cell lines. Conclusions: Bcl-2 is a proto-oncogene known to regulate apoptosis (programmed cell death). Bcl-2 protein is overexpressed in the majority of renal cell carcinomas examined. Bcl-2 overexpression may have a role in tumorigenesis and may explain the relative resistance of renal cell carcinoma to chemotherapeutic agents and to radiation therapy.",
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AU - Fone, Patricia D.

AU - Gandour-Edwards, Regina F

AU - deVere White, Ralph W

AU - Low, Roger

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N2 - Purpose: To investigate the incidence, extent, and distribution of bcl-2 protein expression in human renal cell carcinomas. Materials and Methods: Using immunohistochemical tissue staining techniques, bcl-2 protein expression was analyzed in archival nephrectomy specimens removed for renal cell carcinoma or trauma and in 3 renal cell carcinoma cell lines. Results: Normal kidneys demonstrated bcl-2 immunopositivity primarily within the cytoplasm of distal tubule cells. Only rare and minor staining of the proximal tubular cells, thought to be the origin of renal cell carcinoma, was noted in histologically normal controls and areas adjacent to tumor. In contrast, bcl-2 protein expression was demonstrated in 70% of renal cell carcinomas and in all 3 experimental cell lines. Conclusions: Bcl-2 is a proto-oncogene known to regulate apoptosis (programmed cell death). Bcl-2 protein is overexpressed in the majority of renal cell carcinomas examined. Bcl-2 overexpression may have a role in tumorigenesis and may explain the relative resistance of renal cell carcinoma to chemotherapeutic agents and to radiation therapy.

AB - Purpose: To investigate the incidence, extent, and distribution of bcl-2 protein expression in human renal cell carcinomas. Materials and Methods: Using immunohistochemical tissue staining techniques, bcl-2 protein expression was analyzed in archival nephrectomy specimens removed for renal cell carcinoma or trauma and in 3 renal cell carcinoma cell lines. Results: Normal kidneys demonstrated bcl-2 immunopositivity primarily within the cytoplasm of distal tubule cells. Only rare and minor staining of the proximal tubular cells, thought to be the origin of renal cell carcinoma, was noted in histologically normal controls and areas adjacent to tumor. In contrast, bcl-2 protein expression was demonstrated in 70% of renal cell carcinomas and in all 3 experimental cell lines. Conclusions: Bcl-2 is a proto-oncogene known to regulate apoptosis (programmed cell death). Bcl-2 protein is overexpressed in the majority of renal cell carcinomas examined. Bcl-2 overexpression may have a role in tumorigenesis and may explain the relative resistance of renal cell carcinoma to chemotherapeutic agents and to radiation therapy.

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