Immunoglobulin E-reactive proteins in cashew (Anacardium occidentale) apple juice concentrate

Sarah S. Comstock, Jason M. Robotham, Pallavi Tawde, Harshal Kshirsagar, Shridhar K. Sathe, Kenneth H. Roux, Suzanne S Teuber

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Cashew apple juice has the potential to be a natural source of vitamin C and sugar in processed foods. The juice of the cashew apple is obtained by pressing the fleshy peduncle or receptacle, which forms a rounded apple that sits above the true fruit, the cashew nut. Cashew nut allergy is the second most commonly reported tree nut allergy in the United States. To determine if cashew apple juice contains cashew nut allergens, immunoblotting was performed using a cashew apple juice 6X concentrate that was extracted and further concentrated through dialysis, lyophilization, and resuspension. Serum IgE of individuals allergic to cashew nut bound proteins in the cashew apple juice concentrate extract. For some serum samples, IgE reactivity could be inhibited by preincubation of the serum with cashew nut extract, suggesting the presence of cashew nut-related allergens. Using monoclonal antibodies specific for cashew nut allergens, the concentrate was found to contain Ana o 1 (vicilin) and Ana o 2 (legumin). Neither IgE from cashew nut allergic sera nor the monoclonal antibodies bound any peptides in 5 kDa filtered cashew apple juice concentrate. The cashew apple juice concentrate used in these studies contains proteins with IgE-reactive epitopes, including cashew nut legumin and vicilin. No IgE-binding peptides remained after 5 kDa filtration of the concentrate.

Original languageEnglish (US)
Pages (from-to)5977-5982
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Issue number14
StatePublished - Jul 23 2008


  • Anacardium occidentale
  • Cashew apple
  • Cashew nut
  • Food allergy
  • Ige

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)


Dive into the research topics of 'Immunoglobulin E-reactive proteins in cashew (Anacardium occidentale) apple juice concentrate'. Together they form a unique fingerprint.

Cite this