Immunogenicity of self antigens is unrelated to MHC-binding affinity: T-cell determinant structure of Golli-MBP in the BALB/c mouse

Emanual Michael Maverakis, Richard Mendoza, Scott Southwood, Claudia Raja-Gabaglia, Sara Abromson-Leeman, Anthony T. Campagnoni, Alessandro Sette, Eli E. Sercarz

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The 'classical' myelin basic protein (MBP) exons belong to a much larger unit, termed the 'Golli-MBP' gene. Here we have examined the T-cell determinant structure of the Golli protein region in the BALB/c mouse. Golli p10-24, which was shown to have the strongest affinity for I-A(d), could not induce T-cell activation. Paradoxically, the poorer binding, overlapping p5-19 was effective at inducing T-cell proliferation. Thus, immunogenicity is not necessarily related to the MHC-binding affinity of self-peptides. In addition, MBP: p151-168-specific T cell clones responded only poorly to J37, a Golli-MBP protein, while MBP: 59-76-specific clones responded well to J37. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)315-322
Number of pages8
JournalJournal of Autoimmunity
Volume15
Issue number3
DOIs
StatePublished - 2000

Fingerprint

Myelin Basic Protein
Autoantigens
T-Lymphocytes
Clone Cells
Exons
Proteins
Cell Proliferation
Peptides
Genes

Keywords

  • Autoimmunity
  • Golli-MBP
  • MHC binding affinity
  • Self antigen
  • Tolerance

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Immunogenicity of self antigens is unrelated to MHC-binding affinity : T-cell determinant structure of Golli-MBP in the BALB/c mouse. / Maverakis, Emanual Michael; Mendoza, Richard; Southwood, Scott; Raja-Gabaglia, Claudia; Abromson-Leeman, Sara; Campagnoni, Anthony T.; Sette, Alessandro; Sercarz, Eli E.

In: Journal of Autoimmunity, Vol. 15, No. 3, 2000, p. 315-322.

Research output: Contribution to journalArticle

Maverakis, EM, Mendoza, R, Southwood, S, Raja-Gabaglia, C, Abromson-Leeman, S, Campagnoni, AT, Sette, A & Sercarz, EE 2000, 'Immunogenicity of self antigens is unrelated to MHC-binding affinity: T-cell determinant structure of Golli-MBP in the BALB/c mouse', Journal of Autoimmunity, vol. 15, no. 3, pp. 315-322. https://doi.org/10.1006/jaut.2000.0436
Maverakis, Emanual Michael ; Mendoza, Richard ; Southwood, Scott ; Raja-Gabaglia, Claudia ; Abromson-Leeman, Sara ; Campagnoni, Anthony T. ; Sette, Alessandro ; Sercarz, Eli E. / Immunogenicity of self antigens is unrelated to MHC-binding affinity : T-cell determinant structure of Golli-MBP in the BALB/c mouse. In: Journal of Autoimmunity. 2000 ; Vol. 15, No. 3. pp. 315-322.
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