Immunogenicity of self antigens is unrelated to MHC-binding affinity: T-cell determinant structure of Golli-MBP in the BALB/c mouse

Emanual Michael Maverakis; Richard Mendoza; Scott Southwood; Claudia Raja-Gabaglia; Sara Abromson-Leeman; Anthony T. Campagnoni; Alessandro Sette; Eli E. Sercarz

doi:10.1006/jaut.2000.0436

Immunogenicity of self antigens is unrelated to MHC-binding affinity: T-cell determinant structure of Golli-MBP in the BALB/c mouse

Emanual Michael Maverakis, Richard Mendoza, Scott Southwood, Claudia Raja-Gabaglia, Sara Abromson-Leeman, Anthony T. Campagnoni, Alessandro Sette, Eli E. Sercarz

Dermatology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The 'classical' myelin basic protein (MBP) exons belong to a much larger unit, termed the 'Golli-MBP' gene. Here we have examined the T-cell determinant structure of the Golli protein region in the BALB/c mouse. Golli p10-24, which was shown to have the strongest affinity for I-A(d), could not induce T-cell activation. Paradoxically, the poorer binding, overlapping p5-19 was effective at inducing T-cell proliferation. Thus, immunogenicity is not necessarily related to the MHC-binding affinity of self-peptides. In addition, MBP: p151-168-specific T cell clones responded only poorly to J37, a Golli-MBP protein, while MBP: 59-76-specific clones responded well to J37. (C) 2000 Academic Press.

Original language	English (US)
Pages (from-to)	315-322
Number of pages	8
Journal	Journal of Autoimmunity
Volume	15
Issue number	3
DOIs	https://doi.org/10.1006/jaut.2000.0436
State	Published - 2000

Keywords

Autoimmunity
Golli-MBP
MHC binding affinity
Self antigen
Tolerance

ASJC Scopus subject areas

Immunology
Immunology and Allergy

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10.1006/jaut.2000.0436

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Immunogenicity of self antigens is unrelated to MHC-binding affinity : T-cell determinant structure of Golli-MBP in the BALB/c mouse. / Maverakis, Emanual Michael; Mendoza, Richard; Southwood, Scott; Raja-Gabaglia, Claudia; Abromson-Leeman, Sara; Campagnoni, Anthony T.; Sette, Alessandro; Sercarz, Eli E.

In: Journal of Autoimmunity, Vol. 15, No. 3, 2000, p. 315-322.

Research output: Contribution to journal › Article › peer-review

Maverakis, Emanual Michael ; Mendoza, Richard ; Southwood, Scott ; Raja-Gabaglia, Claudia ; Abromson-Leeman, Sara ; Campagnoni, Anthony T. ; Sette, Alessandro ; Sercarz, Eli E. / Immunogenicity of self antigens is unrelated to MHC-binding affinity : T-cell determinant structure of Golli-MBP in the BALB/c mouse. In: Journal of Autoimmunity. 2000 ; Vol. 15, No. 3. pp. 315-322.

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title = "Immunogenicity of self antigens is unrelated to MHC-binding affinity: T-cell determinant structure of Golli-MBP in the BALB/c mouse",

abstract = "The 'classical' myelin basic protein (MBP) exons belong to a much larger unit, termed the 'Golli-MBP' gene. Here we have examined the T-cell determinant structure of the Golli protein region in the BALB/c mouse. Golli p10-24, which was shown to have the strongest affinity for I-A(d), could not induce T-cell activation. Paradoxically, the poorer binding, overlapping p5-19 was effective at inducing T-cell proliferation. Thus, immunogenicity is not necessarily related to the MHC-binding affinity of self-peptides. In addition, MBP: p151-168-specific T cell clones responded only poorly to J37, a Golli-MBP protein, while MBP: 59-76-specific clones responded well to J37. (C) 2000 Academic Press.",

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AU - Southwood, Scott

AU - Raja-Gabaglia, Claudia

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AB - The 'classical' myelin basic protein (MBP) exons belong to a much larger unit, termed the 'Golli-MBP' gene. Here we have examined the T-cell determinant structure of the Golli protein region in the BALB/c mouse. Golli p10-24, which was shown to have the strongest affinity for I-A(d), could not induce T-cell activation. Paradoxically, the poorer binding, overlapping p5-19 was effective at inducing T-cell proliferation. Thus, immunogenicity is not necessarily related to the MHC-binding affinity of self-peptides. In addition, MBP: p151-168-specific T cell clones responded only poorly to J37, a Golli-MBP protein, while MBP: 59-76-specific clones responded well to J37. (C) 2000 Academic Press.

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Immunogenicity of self antigens is unrelated to MHC-binding affinity: T-cell determinant structure of Golli-MBP in the BALB/c mouse

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Keywords

ASJC Scopus subject areas

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