Abstract
The 'classical' myelin basic protein (MBP) exons belong to a much larger unit, termed the 'Golli-MBP' gene. Here we have examined the T-cell determinant structure of the Golli protein region in the BALB/c mouse. Golli p10-24, which was shown to have the strongest affinity for I-A(d), could not induce T-cell activation. Paradoxically, the poorer binding, overlapping p5-19 was effective at inducing T-cell proliferation. Thus, immunogenicity is not necessarily related to the MHC-binding affinity of self-peptides. In addition, MBP: p151-168-specific T cell clones responded only poorly to J37, a Golli-MBP protein, while MBP: 59-76-specific clones responded well to J37. (C) 2000 Academic Press.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 315-322 |
| Number of pages | 8 |
| Journal | Journal of Autoimmunity |
| Volume | 15 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2000 |
Fingerprint
Keywords
- Autoimmunity
- Golli-MBP
- MHC binding affinity
- Self antigen
- Tolerance
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Immunogenicity of self antigens is unrelated to MHC-binding affinity : T-cell determinant structure of Golli-MBP in the BALB/c mouse. / Maverakis, Emanual Michael; Mendoza, Richard; Southwood, Scott; Raja-Gabaglia, Claudia; Abromson-Leeman, Sara; Campagnoni, Anthony T.; Sette, Alessandro; Sercarz, Eli E.
In: Journal of Autoimmunity, Vol. 15, No. 3, 2000, p. 315-322.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunogenicity of self antigens is unrelated to MHC-binding affinity
T2 - T-cell determinant structure of Golli-MBP in the BALB/c mouse
AU - Maverakis, Emanual Michael
AU - Mendoza, Richard
AU - Southwood, Scott
AU - Raja-Gabaglia, Claudia
AU - Abromson-Leeman, Sara
AU - Campagnoni, Anthony T.
AU - Sette, Alessandro
AU - Sercarz, Eli E.
PY - 2000
Y1 - 2000
N2 - The 'classical' myelin basic protein (MBP) exons belong to a much larger unit, termed the 'Golli-MBP' gene. Here we have examined the T-cell determinant structure of the Golli protein region in the BALB/c mouse. Golli p10-24, which was shown to have the strongest affinity for I-A(d), could not induce T-cell activation. Paradoxically, the poorer binding, overlapping p5-19 was effective at inducing T-cell proliferation. Thus, immunogenicity is not necessarily related to the MHC-binding affinity of self-peptides. In addition, MBP: p151-168-specific T cell clones responded only poorly to J37, a Golli-MBP protein, while MBP: 59-76-specific clones responded well to J37. (C) 2000 Academic Press.
AB - The 'classical' myelin basic protein (MBP) exons belong to a much larger unit, termed the 'Golli-MBP' gene. Here we have examined the T-cell determinant structure of the Golli protein region in the BALB/c mouse. Golli p10-24, which was shown to have the strongest affinity for I-A(d), could not induce T-cell activation. Paradoxically, the poorer binding, overlapping p5-19 was effective at inducing T-cell proliferation. Thus, immunogenicity is not necessarily related to the MHC-binding affinity of self-peptides. In addition, MBP: p151-168-specific T cell clones responded only poorly to J37, a Golli-MBP protein, while MBP: 59-76-specific clones responded well to J37. (C) 2000 Academic Press.
KW - Autoimmunity
KW - Golli-MBP
KW - MHC binding affinity
KW - Self antigen
KW - Tolerance
UR - http://www.scopus.com/inward/record.url?scp=0033711717&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033711717&partnerID=8YFLogxK
U2 - 10.1006/jaut.2000.0436
DO - 10.1006/jaut.2000.0436
M3 - Article
C2 - 11040072
AN - SCOPUS:0033711717
VL - 15
SP - 315
EP - 322
JO - Journal of Autoimmunity
JF - Journal of Autoimmunity
SN - 0896-8411
IS - 3
ER -