Immunogenicity of a vaccine formulated with the Chlamydia trachomatis serovar F, native major outer membrane protein in a nonhuman primate model

Chunmei Cheng, Sukumar Pal, Ilham Bettahi, Kristie L. Oxford, Peter A Barry, Luis M. De la Maza

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

To determine the ability of a vaccine formulated with the genital Chlamydia trachomatis, serovar F, native major outer membrane protein (Ct-F-nMOMP), to induce systemic and mucosal immune responses, rhesus macaques (Macaca mulatta) were immunized three times by the intramuscular (i.m.) and subcutaneous (s.c.) routes using CpG-2395 and Montanide ISA 720 VG, as adjuvants. As controls, another group of M. mulatta was immunized with ovalbumin instead of Ct-F-nMOMP using the same formulation and routes. High levels of Chlamydia-specific IgG and IgA antibodies were detected in plasma, vaginal washes, tears, saliva, and stools from the Ct-F-nMOMP immunized animals. Also, high neutralizing antibody titers were detected in the plasma from these animals. Monkeys immunized with ovalbumin had no detectable Chlamydia-specific antibodies. Furthermore, as measured by a lymphoproliferative assay, significant Chlamydia-specific cell-mediated immune responses were detected in the peripheral blood mononuclear cells (PBMC) from the rhesus macaques vaccinated with Ct-F-nMOMP when compared with the animals immunized with ovalbumin. In addition, the levels of two Th1 cytokines, IFN-γ and TNF-α, were significantly higher in the animals immunized with Ct-F-nMOMP when compared with those from the monkeys immunized with ovalbumin. To our knowledge, this is the first time that mucosal and systemic immune responses have been investigated in a nonhuman primate model using a subunit vaccine from a human genital C. trachomatis serovar.

Original languageEnglish (US)
Pages (from-to)3456-3464
Number of pages9
JournalVaccine
Volume29
Issue number18
DOIs
StatePublished - Apr 18 2011

Fingerprint

Chlamydia trachomatis
outer membrane proteins
Primates
Membrane Proteins
serotypes
animal models
ovalbumin
Ovalbumin
immune response
vaccines
Macaca mulatta
Chlamydia
Mucosal Immunity
genitalia
Haplorhini
monkeys
animals
mucosal immunity
Subunit Vaccines
antibodies

Keywords

  • Chlamydia trachomatis
  • Macaca mulatta
  • Nonhuman primates
  • Systemic and mucosal immune responses
  • Vaccine

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

Immunogenicity of a vaccine formulated with the Chlamydia trachomatis serovar F, native major outer membrane protein in a nonhuman primate model. / Cheng, Chunmei; Pal, Sukumar; Bettahi, Ilham; Oxford, Kristie L.; Barry, Peter A; De la Maza, Luis M.

In: Vaccine, Vol. 29, No. 18, 18.04.2011, p. 3456-3464.

Research output: Contribution to journalArticle

Cheng, Chunmei ; Pal, Sukumar ; Bettahi, Ilham ; Oxford, Kristie L. ; Barry, Peter A ; De la Maza, Luis M. / Immunogenicity of a vaccine formulated with the Chlamydia trachomatis serovar F, native major outer membrane protein in a nonhuman primate model. In: Vaccine. 2011 ; Vol. 29, No. 18. pp. 3456-3464.
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