Immunogenicity of a killed whole Neospora caninum tachyzoite preparation formulated with different adjuvants

A. G. Andrianarivo, L. Choromanski, S. P. McDonough, A. E. Packham, Patricia A Conrad

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

A killed whole Neospora caninum tachyzoite preparation was formulated with various adjuvants and tested for its immunogenicity in cattle. The adjuvants used were: Havlogen, a polymer of acrylic acid cross-linked with polyallylsucrose; Polygen, a non-particulate copolymer; a mixture of Havlogen and Bay R-1005, which is a preparation of free base synthetic glycolipids; and Montanide ISA 773, a water-in-oil emulsion made with a mixture of metabolisable and mineral oils. Immune responses in immunised cattle were compared with those of cattle experimentally infected with culture-derived N. caninum tachyzoites. The overall mean serum IFAT titres were significantly higher (P<0.05) in experimentally infected cattle compared with all immunised cattle. Nonetheless, the maximum antibody titres of the immunised cattle, which were obtained following the third immunisation, were within the range of titres previously described for naturally infected cattle. The overall mean serum IFAT titres were significantly higher (P<0.05) in cattle immunised with the killed tachyzoite preparation formulated with Polygen and with the mixture of Havlogen and Bay R-1005, compared with cattle immunised with the Havlogen- and Montanide-based preparations. Two of the four adjuvant preparations were able to induce cell-mediated immune responses similar to those of the experimentally infected cattle. The Havlogen-adjuvanted tachyzoite preparation elicited N. caninum-specific proliferation of peripheral blood mononuclear cells statistically similar (P=0.095) to that of the infected animals. Peripheral blood mononuclear cells from animals immunised with the Polygen-adjuvanted tachyzoite preparation produced interferon-γ concentrations of similar magnitude (P=0.17) to those from the infected animals. Polygen was one of two adjuvants that elicited the highest antibody responses, and was the only adjuvant that induced interferon-γ levels similar to those of the infected heifers.

Original languageEnglish (US)
Pages (from-to)1613-1625
Number of pages13
JournalInternational Journal for Parasitology
Volume29
Issue number10
DOIs
StatePublished - Oct 1999

Keywords

  • Adjuvants
  • Bovine neosporosis
  • Immune responses
  • Killed whole tachyzoite preparation
  • Neospora caninum

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Immunogenicity of a killed whole Neospora caninum tachyzoite preparation formulated with different adjuvants'. Together they form a unique fingerprint.

  • Cite this