Immunogenicity of a DNA vaccine against herpes B virus in mice and rhesus macaques

Jennifer E. Loomis-Huff, R. Eberle, Kristen M. Lockridge, Gary Rhodes, Peter A Barry

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Herpes B virus (Cercopithecine herpesvirus 1) is endemic in captive macaque populations and poses a serious threat to humans who work with macaques or their tissues. A vaccine that could prevent or limit B virus infection in macaques would lessen occupational risk. To that end, a DNA vaccine plasmid expressing the B virus glycoprotein B (gB) was constructed and tested for immunogenicity in mice and macaques. Intramuscular (IM) or intradermal (ID) immunization in mice elicited antibodies to gB that were relatively stable over time and predominately of the IgG2a isotype. Five juvenile macaques were immunized by either IM + ID (n = 2) or IM (n = 3) routes, with two booster immunizations at 10 and 30 weeks. All five animals developed antibodies to B virus gB, with detectable neutralizing activity in the IM + ID immunized animals. These results demonstrated that DNA immunization can be used to generate an immune response against a B virus glycoprotein in uninfected macaques.

Original languageEnglish (US)
Pages (from-to)4865-4873
Number of pages9
JournalVaccine
Volume19
Issue number32
DOIs
StatePublished - Sep 14 2001

Keywords

  • B virus
  • Cercopithecine herpesvirus 1
  • DNA vaccination
  • Macaca mulatta

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

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