Immunogenic collagen in induced ascitic fluid: concurrent preparation of anti-murine immunoglobulin E.

L. J. Gershwin, J. W. Osebold, Y. C. Zee

Research output: Contribution to journalArticle

Abstract

Antiserum to murine immunoglobulin (Ig) E was produced by inoculation of goats with a pool of partially purified IgE from serum and adjuvant-induced ascitic fluid. Antibodies to collagen were found to be present in the antiserum when the latter was conjugated with fluorescein isothiocyanate and applied to mouse pulmonic tissue. The intense connective tissue fluorescence was eliminated following absorption with mouse collagen. Immunogenic collagen components were presumed to arise in ascitic fluid as a consequence of the adjuvant-induced inflammation. Ascitic fluid is commonly used when large volumes of serum proteins are collected from small mammals. It is suggested that ascitic fluid may not be an ideal antigen source when antiserum is to be used for immunofluorescence studies on tissue.

Original languageEnglish (US)
Pages (from-to)1306-1309
Number of pages4
JournalAmerican Journal of Veterinary Research
Volume42
Issue number8
StatePublished - Aug 1981

ASJC Scopus subject areas

  • veterinary(all)

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