Abstract
Vascularization and the influence of growth hormone on this process were studied during endochondral bone differentiation. Vascular invasion was monitored by immunofluorescent localization of two vascular basement membrane proteins, type IV collagen and laminin, a recently described glycoprotein. In addition, endothelial cell invasion was identified by localization of Factor VIII. New bone formation was induced by subcutaneous implantation of a coarse powder of demineralized rat bone matrix. On days 1 through 9, no vascular elements were detected in the plaque. Mesenchymal cells appeared on day 3, proliferated, and differentiated into cartilage on day 7, while the capillaries proliferated at the periphery of the plaque. Beginning on day 9 with capillary incursion into the center of the plaque, type IV collagen, laminin, and Factor VIII were localized in the invading vascular endothelial cells. Type IV collagen and laminin appeared synchronously in the capillary basement membranes and later in the endothelial lining of cavernous sinusoids. Their distribution pattern was identical. The vascular invasion was prominent by day 14. In hypophysectomized rats, cartilage differentiated normally but vascularization was delayed and reduced. Bone formation was scanty as indicated by 45Ca incorporation. Administration of bovine growth hormone to hypophysectomized recipients restored vascularization and bone formation to the level observed in controls.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 130-136 |
| Number of pages | 7 |
| Journal | Developmental Biology |
| Volume | 75 |
| Issue number | 1 |
| DOIs | |
| State | Published - Mar 1 1980 |
| Externally published | Yes |
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Cite this
Immunofluorescent localization of type IV collagen and laminin during endochondral bone differentiation and regulation by pituitary growth hormone. / Foidart, Jean Michel; Reddi, A Hari.
In: Developmental Biology, Vol. 75, No. 1, 01.03.1980, p. 130-136.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunofluorescent localization of type IV collagen and laminin during endochondral bone differentiation and regulation by pituitary growth hormone
AU - Foidart, Jean Michel
AU - Reddi, A Hari
PY - 1980/3/1
Y1 - 1980/3/1
N2 - Vascularization and the influence of growth hormone on this process were studied during endochondral bone differentiation. Vascular invasion was monitored by immunofluorescent localization of two vascular basement membrane proteins, type IV collagen and laminin, a recently described glycoprotein. In addition, endothelial cell invasion was identified by localization of Factor VIII. New bone formation was induced by subcutaneous implantation of a coarse powder of demineralized rat bone matrix. On days 1 through 9, no vascular elements were detected in the plaque. Mesenchymal cells appeared on day 3, proliferated, and differentiated into cartilage on day 7, while the capillaries proliferated at the periphery of the plaque. Beginning on day 9 with capillary incursion into the center of the plaque, type IV collagen, laminin, and Factor VIII were localized in the invading vascular endothelial cells. Type IV collagen and laminin appeared synchronously in the capillary basement membranes and later in the endothelial lining of cavernous sinusoids. Their distribution pattern was identical. The vascular invasion was prominent by day 14. In hypophysectomized rats, cartilage differentiated normally but vascularization was delayed and reduced. Bone formation was scanty as indicated by 45Ca incorporation. Administration of bovine growth hormone to hypophysectomized recipients restored vascularization and bone formation to the level observed in controls.
AB - Vascularization and the influence of growth hormone on this process were studied during endochondral bone differentiation. Vascular invasion was monitored by immunofluorescent localization of two vascular basement membrane proteins, type IV collagen and laminin, a recently described glycoprotein. In addition, endothelial cell invasion was identified by localization of Factor VIII. New bone formation was induced by subcutaneous implantation of a coarse powder of demineralized rat bone matrix. On days 1 through 9, no vascular elements were detected in the plaque. Mesenchymal cells appeared on day 3, proliferated, and differentiated into cartilage on day 7, while the capillaries proliferated at the periphery of the plaque. Beginning on day 9 with capillary incursion into the center of the plaque, type IV collagen, laminin, and Factor VIII were localized in the invading vascular endothelial cells. Type IV collagen and laminin appeared synchronously in the capillary basement membranes and later in the endothelial lining of cavernous sinusoids. Their distribution pattern was identical. The vascular invasion was prominent by day 14. In hypophysectomized rats, cartilage differentiated normally but vascularization was delayed and reduced. Bone formation was scanty as indicated by 45Ca incorporation. Administration of bovine growth hormone to hypophysectomized recipients restored vascularization and bone formation to the level observed in controls.
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UR - http://www.scopus.com/inward/citedby.url?scp=0018848803&partnerID=8YFLogxK
U2 - 10.1016/0012-1606(80)90149-9
DO - 10.1016/0012-1606(80)90149-9
M3 - Article
C2 - 6154620
AN - SCOPUS:0018848803
VL - 75
SP - 130
EP - 136
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 1
ER -