Abstract
Galectin‐3 is an endogenous soluble lectin within the family called galectins that bind β‐galactosides. Homologs of the protein isolated from different sources were previously designated as IgE‐binding protein (ϵBP), CBP35, CPB30, Mac‐2, RL‐29, RLL, L‐29, and HL‐29. All are now renamed galectin‐3. This lectin is widely distributed in cells and tissues of mice, rats, dogs, hamsters, and humans. Light microscopic immunohistochemistry and ultrastructural immunogold labeling methods were used to determine the distribution of galectin‐3 in human mast cells of several organs, in mast cells developed in vitro from human fetal liver cells, and in human peripheral blood basophils. Immunolabeling for the protein was observed in mast cells from all sources and in basophils. The lectin was detected in the nucleus and/or the cytoplasm. The nuclear labeling was over heterochromatin whereas euchromatin was unlabeled. Cytoplasmic labeling was concentrated over secretory granules. The intensity of staining generally was greater in mast cells of skin when compared with that of mast cells in other locations and with that of basophils. Studies have indicated that in mast cells galectin‐3 may be involved in promoting their adhesion to basal laminae. In this study the localization of galectin‐3 in the secretory granules of human mast cells and basophils suggests that these cells may release this lectin when activated to degranulate. © 1995 Wiley‐Liss, Inc.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 211-219 |
| Number of pages | 9 |
| Journal | The Anatomical Record |
| Volume | 242 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1995 |
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Keywords
- Basophil
- Epsilon binding protein
- Immunogold
- Lectin
- Mast cell
ASJC Scopus subject areas
- Anatomy
Cite this
Immunoelectron microscopic localization of galectin‐3, an IgE binding protein, in human mast cells and basophils. / Craig, Shirley S.; Krishnaswamy, Priya; Irani, Anne‐Marie A.; Kepley, Christopher L.; Liu, Fu-Tong; Schwartz, Lawrence B.
In: The Anatomical Record, Vol. 242, No. 2, 1995, p. 211-219.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunoelectron microscopic localization of galectin‐3, an IgE binding protein, in human mast cells and basophils
AU - Craig, Shirley S.
AU - Krishnaswamy, Priya
AU - Irani, Anne‐Marie A.
AU - Kepley, Christopher L.
AU - Liu, Fu-Tong
AU - Schwartz, Lawrence B.
PY - 1995
Y1 - 1995
N2 - Galectin‐3 is an endogenous soluble lectin within the family called galectins that bind β‐galactosides. Homologs of the protein isolated from different sources were previously designated as IgE‐binding protein (ϵBP), CBP35, CPB30, Mac‐2, RL‐29, RLL, L‐29, and HL‐29. All are now renamed galectin‐3. This lectin is widely distributed in cells and tissues of mice, rats, dogs, hamsters, and humans. Light microscopic immunohistochemistry and ultrastructural immunogold labeling methods were used to determine the distribution of galectin‐3 in human mast cells of several organs, in mast cells developed in vitro from human fetal liver cells, and in human peripheral blood basophils. Immunolabeling for the protein was observed in mast cells from all sources and in basophils. The lectin was detected in the nucleus and/or the cytoplasm. The nuclear labeling was over heterochromatin whereas euchromatin was unlabeled. Cytoplasmic labeling was concentrated over secretory granules. The intensity of staining generally was greater in mast cells of skin when compared with that of mast cells in other locations and with that of basophils. Studies have indicated that in mast cells galectin‐3 may be involved in promoting their adhesion to basal laminae. In this study the localization of galectin‐3 in the secretory granules of human mast cells and basophils suggests that these cells may release this lectin when activated to degranulate. © 1995 Wiley‐Liss, Inc.
AB - Galectin‐3 is an endogenous soluble lectin within the family called galectins that bind β‐galactosides. Homologs of the protein isolated from different sources were previously designated as IgE‐binding protein (ϵBP), CBP35, CPB30, Mac‐2, RL‐29, RLL, L‐29, and HL‐29. All are now renamed galectin‐3. This lectin is widely distributed in cells and tissues of mice, rats, dogs, hamsters, and humans. Light microscopic immunohistochemistry and ultrastructural immunogold labeling methods were used to determine the distribution of galectin‐3 in human mast cells of several organs, in mast cells developed in vitro from human fetal liver cells, and in human peripheral blood basophils. Immunolabeling for the protein was observed in mast cells from all sources and in basophils. The lectin was detected in the nucleus and/or the cytoplasm. The nuclear labeling was over heterochromatin whereas euchromatin was unlabeled. Cytoplasmic labeling was concentrated over secretory granules. The intensity of staining generally was greater in mast cells of skin when compared with that of mast cells in other locations and with that of basophils. Studies have indicated that in mast cells galectin‐3 may be involved in promoting their adhesion to basal laminae. In this study the localization of galectin‐3 in the secretory granules of human mast cells and basophils suggests that these cells may release this lectin when activated to degranulate. © 1995 Wiley‐Liss, Inc.
KW - Basophil
KW - Epsilon binding protein
KW - Immunogold
KW - Lectin
KW - Mast cell
UR - http://www.scopus.com/inward/record.url?scp=0029078421&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029078421&partnerID=8YFLogxK
U2 - 10.1002/ar.1092420210
DO - 10.1002/ar.1092420210
M3 - Article
C2 - 7668406
AN - SCOPUS:0029078421
VL - 242
SP - 211
EP - 219
JO - Anatomical Record
JF - Anatomical Record
SN - 1932-8486
IS - 2
ER -