Immunocytochemical localization of non-NMDA ionotropic excitatory amino acid receptor subunits in human neocortex

James C. Vickers, George W. Huntley, Patrick R. Hof, Joshua Bederson, Javier DeFelipe, John Morrison

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The distribution of immunocytochemically localized subunits that comprise ionotropic non-NMDA excitatory amino acid receptors was examined in human frontal, parietal and temporal association neocortex. AMPA/kainate receptor subunits were identified using a monoclonal antibody (3A11) that recognizes an epitope common to GluR2 and GluR4 [GluR2(4)], as well as polyclonal antisera that recognize GluR2 and GluR3 (GluR2/3). Kainate receptor subunits were identified using a monoclonal antibody (4F5) that recognizes an epitope common to GluR5/6/7. For all three antibodies used, labeling was observed in a large number of neurons throughout the human association neocortex with the highest immunoreactivity present in pyramidal-like neurons, a cellular pattern largely similar to that observed in the monkey neocortex. These data demonstrate the cellular localization patterns for some non-NMDA receptor subunits in human neocortex, details upon which further studies on the roles of these subunits in human neurological diseases can be based.

Original languageEnglish (US)
Pages (from-to)175-180
Number of pages6
JournalBrain Research
Volume671
Issue number1
DOIs
StatePublished - Feb 6 1995
Externally publishedYes

Keywords

  • AMPA/kainate receptor
  • Association neocortex
  • Glutamate receptor
  • Human brain
  • Pyramidal neuron

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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