Immunocytochemical expression of human muscle cell p75 neurotrophin receptor is down-regulated by cyclic adenosine 3',5'-monophosphate

Pierluigi Baron, Elio Scarpini, Silvia Pizzul, Fabrizio Zotti, Giancarlo Conti, David E Pleasure, Guglielmo Scarlato

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

To investigate whether the immunocytochemical expression of low affinity neurotrophin receptor (p75) in human muscle is modulated by increased levels of intracellular cyclic adenosine 3',5'-monophoshate (cAMP), human cultured myogenic cells were treated with cAMP analogues dibutyryl cAMP (dbcAMP 0.5-1 mM) and 8-bromo cAMP (1 mM) or the adenylate cyclase activator forskolin (10- 100 μM). Cultures were processed for indirect immunofluorescence microscopy using an anti-human p75 mAb. The treatment of cultured muscle cells with cAMP analogues or forskolin for two days induced a decrease of immunoreactivity for p75 and a reduction of both myotube formation and morphological cell differentiation. Removal of cAMP derivatives from the medium resulted in a return of immunoreactive cells to the levels of untreated controls. These data indicate that adenylate cyclase is involved in the regulation of human muscle p75.

Original languageEnglish (US)
Pages (from-to)79-82
Number of pages4
JournalNeuroscience Letters
Volume234
Issue number2-3
DOIs
StatePublished - Oct 3 1997
Externally publishedYes

Keywords

  • Adenosine 3',5'- monophosphate
  • Human muscle cells
  • Immunocytochemistry
  • p75

ASJC Scopus subject areas

  • Neuroscience(all)

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