Immunocytochemical expression of human muscle cell p75 neurotrophin receptor is down-regulated by cyclic adenosine 3',5'-monophosphate

Pierluigi Baron, Elio Scarpini, Silvia Pizzul, Fabrizio Zotti, Giancarlo Conti, David E Pleasure, Guglielmo Scarlato

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

To investigate whether the immunocytochemical expression of low affinity neurotrophin receptor (p75) in human muscle is modulated by increased levels of intracellular cyclic adenosine 3',5'-monophoshate (cAMP), human cultured myogenic cells were treated with cAMP analogues dibutyryl cAMP (dbcAMP 0.5-1 mM) and 8-bromo cAMP (1 mM) or the adenylate cyclase activator forskolin (10- 100 μM). Cultures were processed for indirect immunofluorescence microscopy using an anti-human p75 mAb. The treatment of cultured muscle cells with cAMP analogues or forskolin for two days induced a decrease of immunoreactivity for p75 and a reduction of both myotube formation and morphological cell differentiation. Removal of cAMP derivatives from the medium resulted in a return of immunoreactive cells to the levels of untreated controls. These data indicate that adenylate cyclase is involved in the regulation of human muscle p75.

Original languageEnglish (US)
Pages (from-to)79-82
Number of pages4
JournalNeuroscience Letters
Volume234
Issue number2-3
DOIs
StatePublished - Oct 3 1997
Externally publishedYes

Fingerprint

Nerve Growth Factor Receptor
Cyclic AMP
Adenosine
Muscle Cells
Colforsin
Adenylyl Cyclases
Cultured Cells
Muscles
Skeletal Muscle Fibers
Indirect Fluorescent Antibody Technique
Fluorescence Microscopy
Cell Differentiation

Keywords

  • Adenosine 3',5'- monophosphate
  • Human muscle cells
  • Immunocytochemistry
  • p75

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Immunocytochemical expression of human muscle cell p75 neurotrophin receptor is down-regulated by cyclic adenosine 3',5'-monophosphate. / Baron, Pierluigi; Scarpini, Elio; Pizzul, Silvia; Zotti, Fabrizio; Conti, Giancarlo; Pleasure, David E; Scarlato, Guglielmo.

In: Neuroscience Letters, Vol. 234, No. 2-3, 03.10.1997, p. 79-82.

Research output: Contribution to journalArticle

Baron, Pierluigi ; Scarpini, Elio ; Pizzul, Silvia ; Zotti, Fabrizio ; Conti, Giancarlo ; Pleasure, David E ; Scarlato, Guglielmo. / Immunocytochemical expression of human muscle cell p75 neurotrophin receptor is down-regulated by cyclic adenosine 3',5'-monophosphate. In: Neuroscience Letters. 1997 ; Vol. 234, No. 2-3. pp. 79-82.
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