Abstract
To investigate whether the immunocytochemical expression of low affinity neurotrophin receptor (p75) in human muscle is modulated by increased levels of intracellular cyclic adenosine 3',5'-monophoshate (cAMP), human cultured myogenic cells were treated with cAMP analogues dibutyryl cAMP (dbcAMP 0.5-1 mM) and 8-bromo cAMP (1 mM) or the adenylate cyclase activator forskolin (10- 100 μM). Cultures were processed for indirect immunofluorescence microscopy using an anti-human p75 mAb. The treatment of cultured muscle cells with cAMP analogues or forskolin for two days induced a decrease of immunoreactivity for p75 and a reduction of both myotube formation and morphological cell differentiation. Removal of cAMP derivatives from the medium resulted in a return of immunoreactive cells to the levels of untreated controls. These data indicate that adenylate cyclase is involved in the regulation of human muscle p75.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 79-82 |
| Number of pages | 4 |
| Journal | Neuroscience Letters |
| Volume | 234 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - Oct 3 1997 |
| Externally published | Yes |
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Keywords
- Adenosine 3',5'- monophosphate
- Human muscle cells
- Immunocytochemistry
- p75
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Immunocytochemical expression of human muscle cell p75 neurotrophin receptor is down-regulated by cyclic adenosine 3',5'-monophosphate. / Baron, Pierluigi; Scarpini, Elio; Pizzul, Silvia; Zotti, Fabrizio; Conti, Giancarlo; Pleasure, David E; Scarlato, Guglielmo.
In: Neuroscience Letters, Vol. 234, No. 2-3, 03.10.1997, p. 79-82.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunocytochemical expression of human muscle cell p75 neurotrophin receptor is down-regulated by cyclic adenosine 3',5'-monophosphate
AU - Baron, Pierluigi
AU - Scarpini, Elio
AU - Pizzul, Silvia
AU - Zotti, Fabrizio
AU - Conti, Giancarlo
AU - Pleasure, David E
AU - Scarlato, Guglielmo
PY - 1997/10/3
Y1 - 1997/10/3
N2 - To investigate whether the immunocytochemical expression of low affinity neurotrophin receptor (p75) in human muscle is modulated by increased levels of intracellular cyclic adenosine 3',5'-monophoshate (cAMP), human cultured myogenic cells were treated with cAMP analogues dibutyryl cAMP (dbcAMP 0.5-1 mM) and 8-bromo cAMP (1 mM) or the adenylate cyclase activator forskolin (10- 100 μM). Cultures were processed for indirect immunofluorescence microscopy using an anti-human p75 mAb. The treatment of cultured muscle cells with cAMP analogues or forskolin for two days induced a decrease of immunoreactivity for p75 and a reduction of both myotube formation and morphological cell differentiation. Removal of cAMP derivatives from the medium resulted in a return of immunoreactive cells to the levels of untreated controls. These data indicate that adenylate cyclase is involved in the regulation of human muscle p75.
AB - To investigate whether the immunocytochemical expression of low affinity neurotrophin receptor (p75) in human muscle is modulated by increased levels of intracellular cyclic adenosine 3',5'-monophoshate (cAMP), human cultured myogenic cells were treated with cAMP analogues dibutyryl cAMP (dbcAMP 0.5-1 mM) and 8-bromo cAMP (1 mM) or the adenylate cyclase activator forskolin (10- 100 μM). Cultures were processed for indirect immunofluorescence microscopy using an anti-human p75 mAb. The treatment of cultured muscle cells with cAMP analogues or forskolin for two days induced a decrease of immunoreactivity for p75 and a reduction of both myotube formation and morphological cell differentiation. Removal of cAMP derivatives from the medium resulted in a return of immunoreactive cells to the levels of untreated controls. These data indicate that adenylate cyclase is involved in the regulation of human muscle p75.
KW - Adenosine 3',5'- monophosphate
KW - Human muscle cells
KW - Immunocytochemistry
KW - p75
UR - http://www.scopus.com/inward/record.url?scp=0030767087&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030767087&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(97)00640-X
DO - 10.1016/S0304-3940(97)00640-X
M3 - Article
C2 - 9364502
AN - SCOPUS:0030767087
VL - 234
SP - 79
EP - 82
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 2-3
ER -