Immunocytochemical distribution of mouse mammary tumor virus antigens in BALB/cfC3H mammary epithelium

Judith A. St. George, Robert Cardiff, Lawrence J.T. Young, Leslie J. Faulkin

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20 Scopus citations

Abstract

The distribution of mouse mammary tumor virus (MuMTV) antigens was studied in normal, preneoplastlc, and neoplastic mammary eplthelia from female BALB/cfC3H mice with the use of a polyvalent anti-MuMTV serum and indirect Immunoperoxidase techniques. The MuMTV antigens were on the apical surface or in focal cytoplasmic aggregates or diffused throughout the infected cells. As much as 70% of all cells in adenocarcinomas and as much as 100% of all cells in preneoplastlc hyperplastic alveolar nodules contained MuMTV antigens. Comparable percentages of cells from mammary glands of multl-parous mice were MuMTV-positlve. Some mammary tissues of nulliparous and primiparous mice did not contain detectable MuMTV antigen. The MuMTV antigen-containing cells In lactat-Ing mammary glands tended to be in discrete lobuloalveolar clusters surrounded by antigen-negative alveoli. The percentage of MuMTV-positlve cells in a given gland was proportional to the amount of virus found in the animal’s milk.

Original languageEnglish (US)
Pages (from-to)813-820
Number of pages8
JournalJournal of the National Cancer Institute
Volume63
Issue number3
DOIs
StatePublished - Jan 1 1979

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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