Immunochemical identification and subcellular distribution of the α1A subunits of brain calcium channels

Ruth E. Westenbroek, Takashi Sakurai, Elicia M. Elliott, Johannes W Hell, Terry V B Starr, Terry P. Snutch, William A. Catterall

Research output: Contribution to journalArticle

466 Citations (Scopus)

Abstract

A site-directed anti-peptide antibody (anti-CNA1) directed against the α1, subunit of class A calcium channels (α1A) recognized a protein of approximately 190-200 kDa in immunoblot and immunoprecipitation analyses of rat brain glycoproteins. Calcium channels recognized by anti-CNA1 were distributed throughout the brain with a high concentration in the cerebellum. Calcium channels having α1A subunits were concentrated in presynaptic terminals making synapses on cell bodies and on dendritic shafts and spines of many classes of neurons and were especially prominent in the synapses of the parallel fibers of cerebellar granule cells on Purkinje neurons where their localization in presynaptic terminals was confirmed by double labeling with the synaptic membrane protein syntaxin or the microinjected postsynaptic marker Neurobiotin. They were present in lower density in the surface membrane of dendrites of most major classes of neurons. There was substantial labeling of Purkinje cell bodies, but less intense staining of the cell bodies of hippocampal pyramidal neurons, layer V pyramidal neurons in the dorsal cortex, and most other classes of neurons in the forebrain and cerebellum. Scattered cell bodies elsewhere in the brain were labeled at low levels. These results define a unique pattern of localization of class A calcium channels in the cell bodies, dendrites, and presynaptic terminals of most central neurons. Compared to class B N-type calcium channels, class A calcium channels are concentrated in a larger number of presynaptic nerve terminals implying a more prominent role in neurotransmitter release at many central synapses.

Original languageEnglish (US)
Pages (from-to)6403-6418
Number of pages16
JournalJournal of Neuroscience
Volume15
Issue number10
StatePublished - Oct 1995
Externally publishedYes

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Calcium Channels
Presynaptic Terminals
Synapses
Brain
Neurons
Pyramidal Cells
Purkinje Cells
Dendrites
Cerebellum
N-Type Calcium Channels
Qa-SNARE Proteins
Synaptic Membranes
Dendritic Spines
Prosencephalon
Immunoprecipitation
Neurotransmitter Agents
Anti-Idiotypic Antibodies
Glycoproteins
Membrane Proteins
Cell Body

Keywords

  • Calcium channel
  • Dendrites
  • Immunocytochemistry
  • Ion channel
  • Purkinje cell
  • Synaptic terminals

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Westenbroek, R. E., Sakurai, T., Elliott, E. M., Hell, J. W., Starr, T. V. B., Snutch, T. P., & Catterall, W. A. (1995). Immunochemical identification and subcellular distribution of the α1A subunits of brain calcium channels. Journal of Neuroscience, 15(10), 6403-6418.

Immunochemical identification and subcellular distribution of the α1A subunits of brain calcium channels. / Westenbroek, Ruth E.; Sakurai, Takashi; Elliott, Elicia M.; Hell, Johannes W; Starr, Terry V B; Snutch, Terry P.; Catterall, William A.

In: Journal of Neuroscience, Vol. 15, No. 10, 10.1995, p. 6403-6418.

Research output: Contribution to journalArticle

Westenbroek, RE, Sakurai, T, Elliott, EM, Hell, JW, Starr, TVB, Snutch, TP & Catterall, WA 1995, 'Immunochemical identification and subcellular distribution of the α1A subunits of brain calcium channels', Journal of Neuroscience, vol. 15, no. 10, pp. 6403-6418.
Westenbroek RE, Sakurai T, Elliott EM, Hell JW, Starr TVB, Snutch TP et al. Immunochemical identification and subcellular distribution of the α1A subunits of brain calcium channels. Journal of Neuroscience. 1995 Oct;15(10):6403-6418.
Westenbroek, Ruth E. ; Sakurai, Takashi ; Elliott, Elicia M. ; Hell, Johannes W ; Starr, Terry V B ; Snutch, Terry P. ; Catterall, William A. / Immunochemical identification and subcellular distribution of the α1A subunits of brain calcium channels. In: Journal of Neuroscience. 1995 ; Vol. 15, No. 10. pp. 6403-6418.
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