Abstract
A recently described 170,000-M(r) glycoprotein, specific to peripheral nervous system (PNS) myelin, was purified from rat PNS myelin by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and used to immunize guinea pigs and rabbits. The resultant antisera proved specific for 170,000-M(r) glycoprotein by enzyme-linked immunosorbent assay, by immunoprecipitation of the appropriate peptide from solubilized PNS myelin, and by immunoblot analysis of rat PNS myelin. The anti-rat 170,000-M(r) glycoprotein antisera crossreacted with proteins of similar molecular weight in human and bovine PNS myelin, but such proteins were not detected in human or rat CNS myelin or other rat tissues. The 170,000-M(r) glycoprotein was also detected by this immunoblot procedure in recently isolated rat Schwann cells but not in those kept in culture for ≥3 days. By indirect immunofluorescent microscopy, anti-rat 170,000-M(r) glycoprotein antibody bound to rat PNS myelin sheaths but not to other rat tissues. Together these studies indicate that the 170,000-M(r) glycoprotein is specific to PNS myelin of several species and that a neuronal influence may be required for its expression by Schwann cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 811-818 |
| Number of pages | 8 |
| Journal | Journal of Neurochemistry |
| Volume | 47 |
| Issue number | 3 |
| State | Published - 1986 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Cellular and Molecular Neuroscience
Cite this
Immunochemical characterization of peripheral nervous system myelin 170,000-M(r) glycoprotein. / Shuman, S.; Hardy, M.; Pleasure, David E.
In: Journal of Neurochemistry, Vol. 47, No. 3, 1986, p. 811-818.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunochemical characterization of peripheral nervous system myelin 170,000-M(r) glycoprotein
AU - Shuman, S.
AU - Hardy, M.
AU - Pleasure, David E
PY - 1986
Y1 - 1986
N2 - A recently described 170,000-M(r) glycoprotein, specific to peripheral nervous system (PNS) myelin, was purified from rat PNS myelin by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and used to immunize guinea pigs and rabbits. The resultant antisera proved specific for 170,000-M(r) glycoprotein by enzyme-linked immunosorbent assay, by immunoprecipitation of the appropriate peptide from solubilized PNS myelin, and by immunoblot analysis of rat PNS myelin. The anti-rat 170,000-M(r) glycoprotein antisera crossreacted with proteins of similar molecular weight in human and bovine PNS myelin, but such proteins were not detected in human or rat CNS myelin or other rat tissues. The 170,000-M(r) glycoprotein was also detected by this immunoblot procedure in recently isolated rat Schwann cells but not in those kept in culture for ≥3 days. By indirect immunofluorescent microscopy, anti-rat 170,000-M(r) glycoprotein antibody bound to rat PNS myelin sheaths but not to other rat tissues. Together these studies indicate that the 170,000-M(r) glycoprotein is specific to PNS myelin of several species and that a neuronal influence may be required for its expression by Schwann cells.
AB - A recently described 170,000-M(r) glycoprotein, specific to peripheral nervous system (PNS) myelin, was purified from rat PNS myelin by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and used to immunize guinea pigs and rabbits. The resultant antisera proved specific for 170,000-M(r) glycoprotein by enzyme-linked immunosorbent assay, by immunoprecipitation of the appropriate peptide from solubilized PNS myelin, and by immunoblot analysis of rat PNS myelin. The anti-rat 170,000-M(r) glycoprotein antisera crossreacted with proteins of similar molecular weight in human and bovine PNS myelin, but such proteins were not detected in human or rat CNS myelin or other rat tissues. The 170,000-M(r) glycoprotein was also detected by this immunoblot procedure in recently isolated rat Schwann cells but not in those kept in culture for ≥3 days. By indirect immunofluorescent microscopy, anti-rat 170,000-M(r) glycoprotein antibody bound to rat PNS myelin sheaths but not to other rat tissues. Together these studies indicate that the 170,000-M(r) glycoprotein is specific to PNS myelin of several species and that a neuronal influence may be required for its expression by Schwann cells.
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M3 - Article
VL - 47
SP - 811
EP - 818
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
SN - 0022-3042
IS - 3
ER -