Immunochemical characterization of peripheral nervous system myelin 170,000-M(r) glycoprotein

S. Shuman, M. Hardy, David E Pleasure

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A recently described 170,000-M(r) glycoprotein, specific to peripheral nervous system (PNS) myelin, was purified from rat PNS myelin by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and used to immunize guinea pigs and rabbits. The resultant antisera proved specific for 170,000-M(r) glycoprotein by enzyme-linked immunosorbent assay, by immunoprecipitation of the appropriate peptide from solubilized PNS myelin, and by immunoblot analysis of rat PNS myelin. The anti-rat 170,000-M(r) glycoprotein antisera crossreacted with proteins of similar molecular weight in human and bovine PNS myelin, but such proteins were not detected in human or rat CNS myelin or other rat tissues. The 170,000-M(r) glycoprotein was also detected by this immunoblot procedure in recently isolated rat Schwann cells but not in those kept in culture for ≥3 days. By indirect immunofluorescent microscopy, anti-rat 170,000-M(r) glycoprotein antibody bound to rat PNS myelin sheaths but not to other rat tissues. Together these studies indicate that the 170,000-M(r) glycoprotein is specific to PNS myelin of several species and that a neuronal influence may be required for its expression by Schwann cells.

Original languageEnglish (US)
Pages (from-to)811-818
Number of pages8
JournalJournal of Neurochemistry
Volume47
Issue number3
StatePublished - 1986
Externally publishedYes

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Peripheral Nervous System
Neurology
Myelin Sheath
Rats
Glycoproteins
Schwann Cells
Immune Sera
Cells
Tissue
Myelin Proteins
Immunosorbents
Electrophoresis
Cell culture
Immunoprecipitation
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Microscopy
Assays
Microscopic examination
Guinea Pigs

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Immunochemical characterization of peripheral nervous system myelin 170,000-M(r) glycoprotein. / Shuman, S.; Hardy, M.; Pleasure, David E.

In: Journal of Neurochemistry, Vol. 47, No. 3, 1986, p. 811-818.

Research output: Contribution to journalArticle

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