Immunochemical characterization of a mr 115 Lens fiber cell-specific extrinsic membrane protein

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Monoclonal and polyclonal antibodies have been produced against a lens fiber cell extrinsic membrane protein, with a relative molecular weight of approximately 115 kd. Enzyme Linked Immunosorbent Assays (ELISA) of retina, ciliary body-iris, liver, and skeletal muscle, utilizing these antibodies, suggest that the antigen is unique to the lens. Immunocytochemistry indicates that the antigen is present only in the differentiated fiber cell, and is absent from the lens epithelium. Further, immunocytochemical reactivity is predominantly associated with the fiber cell plasma membrane. However, sequential extraction of fiber cell homogenate, followed by quantitative, competitive ELISA analysis, indicates that most of the antigen is recovered in the neutral buffer extract. ELISA analysis using monoclonal antibodies indicates that an analogous antigen is present in human and rabbit lenses. On the basis of these results we characterize this antigen as a conserved extrinsic membrane protein, which is unique to the differentiated lens fiber cell. The relationship of this antigen to a previously described Mr 95 beaded filament-associated protein is discussed.

Original languageEnglish (US)
Pages (from-to)1243-1253
Number of pages11
JournalCurrent Eye Research
Volume7
Issue number12
DOIs
StatePublished - 1988

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Lenses
Membrane Proteins
Antigens
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Cell Membrane
Ciliary Body
Iris
Retina
Buffers
Skeletal Muscle
Epithelium
Molecular Weight
Immunohistochemistry
Rabbits
Antibodies
Liver
Proteins

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Immunochemical characterization of a mr 115 Lens fiber cell-specific extrinsic membrane protein. / FitzGerald, Paul G.

In: Current Eye Research, Vol. 7, No. 12, 1988, p. 1243-1253.

Research output: Contribution to journalArticle

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