Immunoassays for pesticide monitoring

Ingrid Wengatz, Ferenc Szurdoki, Anand R. Swamy, Lawrence Evans, Gabor Patonay, Eric Stimmann, Michael Delwiche, Donald Stoutamire, Shirley J. Gee, Bruce Hammock

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

This study compares two formats of rapid assays for the detection of pesticides (bromacil and pyrethroid based metabolites): enzyme linked immunosorbent assay (ELISA) and immunoassay with near- infrared (NIR) fluorescence detection. NIR dye immunoassay (NIRDIA) measurements were carried out by using two different instruments, both having a silicon photodiode as the detector and a laser diode for excitation. ELISA and NIRDIA were performed in a tracer format, where the specific antibody is bound to the surface of a microtiter plate well and the tracer with enzyme or fluorescent dye label competes with the analyte for the antibody binding site. It was demonstrated that the NIRDIA is at least as sensitive as the ELISA. Both assays detect pesticides in the μg/L (ppb) range. Hapten-macromolecule-NIR dye-conjugates have been synthesized with various biopolymers (e.g., proteins) as carriers. The use of carrier macromolecules enables convenient purification of the cyanine dye derivatives. The mild conjugation method of the dye is based on isothiocyanate chemistry.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Place of PublicationBellingham, WA, United States
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages408-416
Number of pages9
Volume2388
ISBN (Print)0819417351
StatePublished - 1995
EventAdvances in Fluorescence Sensing Technology II - San Jose, CA, USA
Duration: Feb 6 1995Feb 8 1995

Other

OtherAdvances in Fluorescence Sensing Technology II
CitySan Jose, CA, USA
Period2/6/952/8/95

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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  • Cite this

    Wengatz, I., Szurdoki, F., Swamy, A. R., Evans, L., Patonay, G., Stimmann, E., Delwiche, M., Stoutamire, D., Gee, S. J., & Hammock, B. (1995). Immunoassays for pesticide monitoring. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 2388, pp. 408-416). Society of Photo-Optical Instrumentation Engineers.