Abstract
This study compares two formats of rapid assays for the detection of pesticides (bromacil and pyrethroid based metabolites): enzyme linked immunosorbent assay (ELISA) and immunoassay with near- infrared (NIR) fluorescence detection. NIR dye immunoassay (NIRDIA) measurements were carried out by using two different instruments, both having a silicon photodiode as the detector and a laser diode for excitation. ELISA and NIRDIA were performed in a tracer format, where the specific antibody is bound to the surface of a microtiter plate well and the tracer with enzyme or fluorescent dye label competes with the analyte for the antibody binding site. It was demonstrated that the NIRDIA is at least as sensitive as the ELISA. Both assays detect pesticides in the μg/L (ppb) range. Hapten-macromolecule-NIR dye-conjugates have been synthesized with various biopolymers (e.g., proteins) as carriers. The use of carrier macromolecules enables convenient purification of the cyanine dye derivatives. The mild conjugation method of the dye is based on isothiocyanate chemistry.
| Original language | English (US) |
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| Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
| Place of Publication | Bellingham, WA, United States |
| Publisher | Society of Photo-Optical Instrumentation Engineers |
| Pages | 408-416 |
| Number of pages | 9 |
| Volume | 2388 |
| ISBN (Print) | 0819417351 |
| State | Published - 1995 |
| Event | Advances in Fluorescence Sensing Technology II - San Jose, CA, USA Duration: Feb 6 1995 → Feb 8 1995 |
Other
| Other | Advances in Fluorescence Sensing Technology II |
|---|---|
| City | San Jose, CA, USA |
| Period | 2/6/95 → 2/8/95 |
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Condensed Matter Physics