Immunoassay detection of drugs in racing horses. XI. ELISA and RIA detection of fentanyl, alfentanil, sufentanil and carfentanil in equine blood and urine

T. Tobin, S. Kwiatkowski, D. S. Watt, H. H. Tai, C. L. Tai, W. E. Woods, J. P. Goodman, D. G. Taylor, T. J. Weckman, J. M. Yang, J. D. Tai, Scott D Stanley, T. Wood, S. L. Chang, J. W. Blake, J. McDonald, S. Wie, C. A. Prange, C. E. Uboh

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

We have developed and evaluated a one-step enzyme-linked immunosorbent assay (ELISA) test for sufentanil and a 125I radioimmunoassay test for alfentanil as part of a panel of pre- and post-race tests for narcotic analgesics in racing horses. Our sufentanil ELISA test detects sufentanil with an I-50 of about 0.5 ng/ml. The test is rapid and economical in that it can be read with an inexpensive spectrophotometer, or even by eye. The test readily detects the presence of sufentanil or its metabolites in equine blood and urine from 1 to 24 hours respectively after administration of therapeutic or sub-therapeutic doses of this drug. Our sufentanil assay also cross-reacts with fentanyl, the methylated analogs of fentanyl (designer fentanyls), and carfentanil and detected these drugs in urine for several hours after their administration to horses. It does not, however, cross-react significantly with alfentanil. We have also developed an 125I radioimmunoassay for alfentanil. This test allows detection of alfentanil in blood and urine of horses for up to 4 hours after administration of this drug. As such, these tests are capable of improving the quality and reducing the cost of pre-race and post-race testing for fentanyl, sufentanil, carfentanil and alfentanil and a number of their congeners in racing horses. Similarly, these tests are capable of screening for these drugs in human drug abuse monitoring.

Original languageEnglish (US)
Pages (from-to)129-152
Number of pages24
JournalResearch Communications in Chemical Pathology and Pharmacology
Volume63
Issue number1
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

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    Tobin, T., Kwiatkowski, S., Watt, D. S., Tai, H. H., Tai, C. L., Woods, W. E., Goodman, J. P., Taylor, D. G., Weckman, T. J., Yang, J. M., Tai, J. D., Stanley, S. D., Wood, T., Chang, S. L., Blake, J. W., McDonald, J., Wie, S., Prange, C. A., & Uboh, C. E. (1989). Immunoassay detection of drugs in racing horses. XI. ELISA and RIA detection of fentanyl, alfentanil, sufentanil and carfentanil in equine blood and urine. Research Communications in Chemical Pathology and Pharmacology, 63(1), 129-152.