Immunoassay detection of drugs in racing horses. X. Detection of phenylbutazone in equine blood and urine by particle concentration fluoroimmunoassay and elisa

S. Kwiatkowski, J. P. Goodman, Scott D Stanley, C. L. Tai, J. M. Yang, T. Wood, L. Sturma, W. E. Woods, Z. G. Tian, S. Bertram, H. H. Tai, T. J. Weckman, S. L. Chang, J. W. Blake, D. S. Watt, T. Tobin, J. McDonald, S. Wie, R. A. Chung

Research output: Contribution to journalArticle

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Abstract

We have developed Particle Concentration Fluorescence Immunoassay (PCFIA) and Enzyme Linked Immunosorbent Assay (ELISA) tests for phenylbutazone as part of a panel of immunoassay tests for drugs in racing horses. Since phenylbutazone is a legal drug in most American racing jurisdictions, the primary use for this test is likely to be quantitation of plasma levels of PB. Our PCFIA test had an I-50 for phenylbutazone of about 1 μg/ml, close to the generally used 5 μg/ml plasma limit for phenylbutazone. Our ELISA test is substantially more sensitive to PB, with an I-50 for phenylbutazone of about 10 ng/ml. Both tests are based on our anti-phenylbutazone antibody, which shows limited cross-reactivity with oxyphenbutazone and does not cross-react significantly with other acidic drugs used in horses. Quantitation of phenylbutazone in equine blood and urine by these assays correlates well with gas chromatography/mass spectrometry quantitation of phenylbutazone. Both assays are sufficiently sensitive to detect the presence of phenylbutazone or its metabolites in equine urine for up to 48 hours, and in equine plasma for up to 24 hours after therapeutic doses of this drug. These tests are capable of providing rapid, sensitive, and economical methods for racing jurisdictions to detect and estimate plasma and/or urinary concentrations of phenylbutazone. Other uses include pre-race and pre-purchase screening for unauthorized blood levels of phenylbutazone and screening for phenylbutazone toxicity.

Original languageEnglish (US)
Pages (from-to)123-138
Number of pages16
JournalResearch Communications in Substances of Abuse
Volume10
Issue number2
StatePublished - 1989
Externally publishedYes

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Fluoroimmunoassay
Phenylbutazone
Immunoassay
Horses
Urine
Pharmaceutical Preparations
Fluorescence
Enzyme-Linked Immunosorbent Assay
Oxyphenbutazone
Gas Chromatography-Mass Spectrometry
Anti-Idiotypic Antibodies

ASJC Scopus subject areas

  • Medicine (miscellaneous)

Cite this

Immunoassay detection of drugs in racing horses. X. Detection of phenylbutazone in equine blood and urine by particle concentration fluoroimmunoassay and elisa. / Kwiatkowski, S.; Goodman, J. P.; Stanley, Scott D; Tai, C. L.; Yang, J. M.; Wood, T.; Sturma, L.; Woods, W. E.; Tian, Z. G.; Bertram, S.; Tai, H. H.; Weckman, T. J.; Chang, S. L.; Blake, J. W.; Watt, D. S.; Tobin, T.; McDonald, J.; Wie, S.; Chung, R. A.

In: Research Communications in Substances of Abuse, Vol. 10, No. 2, 1989, p. 123-138.

Research output: Contribution to journalArticle

Kwiatkowski, S, Goodman, JP, Stanley, SD, Tai, CL, Yang, JM, Wood, T, Sturma, L, Woods, WE, Tian, ZG, Bertram, S, Tai, HH, Weckman, TJ, Chang, SL, Blake, JW, Watt, DS, Tobin, T, McDonald, J, Wie, S & Chung, RA 1989, 'Immunoassay detection of drugs in racing horses. X. Detection of phenylbutazone in equine blood and urine by particle concentration fluoroimmunoassay and elisa', Research Communications in Substances of Abuse, vol. 10, no. 2, pp. 123-138.
Kwiatkowski, S. ; Goodman, J. P. ; Stanley, Scott D ; Tai, C. L. ; Yang, J. M. ; Wood, T. ; Sturma, L. ; Woods, W. E. ; Tian, Z. G. ; Bertram, S. ; Tai, H. H. ; Weckman, T. J. ; Chang, S. L. ; Blake, J. W. ; Watt, D. S. ; Tobin, T. ; McDonald, J. ; Wie, S. ; Chung, R. A. / Immunoassay detection of drugs in racing horses. X. Detection of phenylbutazone in equine blood and urine by particle concentration fluoroimmunoassay and elisa. In: Research Communications in Substances of Abuse. 1989 ; Vol. 10, No. 2. pp. 123-138.
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abstract = "We have developed Particle Concentration Fluorescence Immunoassay (PCFIA) and Enzyme Linked Immunosorbent Assay (ELISA) tests for phenylbutazone as part of a panel of immunoassay tests for drugs in racing horses. Since phenylbutazone is a legal drug in most American racing jurisdictions, the primary use for this test is likely to be quantitation of plasma levels of PB. Our PCFIA test had an I-50 for phenylbutazone of about 1 μg/ml, close to the generally used 5 μg/ml plasma limit for phenylbutazone. Our ELISA test is substantially more sensitive to PB, with an I-50 for phenylbutazone of about 10 ng/ml. Both tests are based on our anti-phenylbutazone antibody, which shows limited cross-reactivity with oxyphenbutazone and does not cross-react significantly with other acidic drugs used in horses. Quantitation of phenylbutazone in equine blood and urine by these assays correlates well with gas chromatography/mass spectrometry quantitation of phenylbutazone. Both assays are sufficiently sensitive to detect the presence of phenylbutazone or its metabolites in equine urine for up to 48 hours, and in equine plasma for up to 24 hours after therapeutic doses of this drug. These tests are capable of providing rapid, sensitive, and economical methods for racing jurisdictions to detect and estimate plasma and/or urinary concentrations of phenylbutazone. Other uses include pre-race and pre-purchase screening for unauthorized blood levels of phenylbutazone and screening for phenylbutazone toxicity.",
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AU - Kwiatkowski, S.

AU - Goodman, J. P.

AU - Stanley, Scott D

AU - Tai, C. L.

AU - Yang, J. M.

AU - Wood, T.

AU - Sturma, L.

AU - Woods, W. E.

AU - Tian, Z. G.

AU - Bertram, S.

AU - Tai, H. H.

AU - Weckman, T. J.

AU - Chang, S. L.

AU - Blake, J. W.

AU - Watt, D. S.

AU - Tobin, T.

AU - McDonald, J.

AU - Wie, S.

AU - Chung, R. A.

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N2 - We have developed Particle Concentration Fluorescence Immunoassay (PCFIA) and Enzyme Linked Immunosorbent Assay (ELISA) tests for phenylbutazone as part of a panel of immunoassay tests for drugs in racing horses. Since phenylbutazone is a legal drug in most American racing jurisdictions, the primary use for this test is likely to be quantitation of plasma levels of PB. Our PCFIA test had an I-50 for phenylbutazone of about 1 μg/ml, close to the generally used 5 μg/ml plasma limit for phenylbutazone. Our ELISA test is substantially more sensitive to PB, with an I-50 for phenylbutazone of about 10 ng/ml. Both tests are based on our anti-phenylbutazone antibody, which shows limited cross-reactivity with oxyphenbutazone and does not cross-react significantly with other acidic drugs used in horses. Quantitation of phenylbutazone in equine blood and urine by these assays correlates well with gas chromatography/mass spectrometry quantitation of phenylbutazone. Both assays are sufficiently sensitive to detect the presence of phenylbutazone or its metabolites in equine urine for up to 48 hours, and in equine plasma for up to 24 hours after therapeutic doses of this drug. These tests are capable of providing rapid, sensitive, and economical methods for racing jurisdictions to detect and estimate plasma and/or urinary concentrations of phenylbutazone. Other uses include pre-race and pre-purchase screening for unauthorized blood levels of phenylbutazone and screening for phenylbutazone toxicity.

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