Abstract
We have developed a Particle Concentration Fluorescence Immunoassay (PCFIA) for cocaine as part of a panel of pre- and post-race tests for illegal drugs in racing horses. This PCFIA test is very sensitive, with an I-50 for cocaine of about 2.5 ng/mL. It does not cross-react significantly with scopolamine, atropine, procaine, or ecgonine. Benzoylecgonine cross-reactivity is less than 10%. The test is rapid, and a normal complement of pre-race tests can be analyzed within one hour. This test detects the presence of cocaine or its metabolites in equine urine for up to 48 hours after a dose of 10 mg/horse, and for at least 72 hours if the dose is 80 mg or larger. In serum, the test detects cocaine for at least 8 hours and likely longer at the 10 mg/horse dosing level. The test is many times more sensitive than thin layer chromatographic tests for cocaine, and is capable of improving the quality of both pre- and post-race testing for cocaine in horses. On initial introduction into post-race urine screening the test flagged 2 of 83 samples 'suspect' for cocaine, which samples subsequently confirmed positive for cocaine by mass spectrometry. Because of the ready availability of cocaine and the small doses (less than 10 mg/horse) that are effective in racing horses, a simple, inexpensive, and sensitive test for cocaine is essential for control of abuse of this drug in racing horses.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1-14 |
| Number of pages | 14 |
| Journal | Research Communications in Substances of Abuse |
| Volume | 10 |
| Issue number | 1 |
| State | Published - 1989 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine (miscellaneous)
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Immunoassay detection of drugs in racing horses. VIII. Detection of cocaine in equine blood and urine by particle concentration fluorescence immunoassay. / Prange, C. A.; Brockus, C.; Wie, S.; Chung, R. A.; Brecht, J. M.; Conner, J. C.; Dahl, P. A.; Lewis, E. L.; McDonald, J.; Kalita, S.; Ozog, F. J.; Green, M. T.; Kwiatkowski, S.; Goodman, J. P.; Sturma, L.; Stanley, Scott D; Yang, J. M.; Wood, T.; Henry, P.
In: Research Communications in Substances of Abuse, Vol. 10, No. 1, 1989, p. 1-14.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunoassay detection of drugs in racing horses. VIII. Detection of cocaine in equine blood and urine by particle concentration fluorescence immunoassay
AU - Prange, C. A.
AU - Brockus, C.
AU - Wie, S.
AU - Chung, R. A.
AU - Brecht, J. M.
AU - Conner, J. C.
AU - Dahl, P. A.
AU - Lewis, E. L.
AU - McDonald, J.
AU - Kalita, S.
AU - Ozog, F. J.
AU - Green, M. T.
AU - Kwiatkowski, S.
AU - Goodman, J. P.
AU - Sturma, L.
AU - Stanley, Scott D
AU - Yang, J. M.
AU - Wood, T.
AU - Henry, P.
PY - 1989
Y1 - 1989
N2 - We have developed a Particle Concentration Fluorescence Immunoassay (PCFIA) for cocaine as part of a panel of pre- and post-race tests for illegal drugs in racing horses. This PCFIA test is very sensitive, with an I-50 for cocaine of about 2.5 ng/mL. It does not cross-react significantly with scopolamine, atropine, procaine, or ecgonine. Benzoylecgonine cross-reactivity is less than 10%. The test is rapid, and a normal complement of pre-race tests can be analyzed within one hour. This test detects the presence of cocaine or its metabolites in equine urine for up to 48 hours after a dose of 10 mg/horse, and for at least 72 hours if the dose is 80 mg or larger. In serum, the test detects cocaine for at least 8 hours and likely longer at the 10 mg/horse dosing level. The test is many times more sensitive than thin layer chromatographic tests for cocaine, and is capable of improving the quality of both pre- and post-race testing for cocaine in horses. On initial introduction into post-race urine screening the test flagged 2 of 83 samples 'suspect' for cocaine, which samples subsequently confirmed positive for cocaine by mass spectrometry. Because of the ready availability of cocaine and the small doses (less than 10 mg/horse) that are effective in racing horses, a simple, inexpensive, and sensitive test for cocaine is essential for control of abuse of this drug in racing horses.
AB - We have developed a Particle Concentration Fluorescence Immunoassay (PCFIA) for cocaine as part of a panel of pre- and post-race tests for illegal drugs in racing horses. This PCFIA test is very sensitive, with an I-50 for cocaine of about 2.5 ng/mL. It does not cross-react significantly with scopolamine, atropine, procaine, or ecgonine. Benzoylecgonine cross-reactivity is less than 10%. The test is rapid, and a normal complement of pre-race tests can be analyzed within one hour. This test detects the presence of cocaine or its metabolites in equine urine for up to 48 hours after a dose of 10 mg/horse, and for at least 72 hours if the dose is 80 mg or larger. In serum, the test detects cocaine for at least 8 hours and likely longer at the 10 mg/horse dosing level. The test is many times more sensitive than thin layer chromatographic tests for cocaine, and is capable of improving the quality of both pre- and post-race testing for cocaine in horses. On initial introduction into post-race urine screening the test flagged 2 of 83 samples 'suspect' for cocaine, which samples subsequently confirmed positive for cocaine by mass spectrometry. Because of the ready availability of cocaine and the small doses (less than 10 mg/horse) that are effective in racing horses, a simple, inexpensive, and sensitive test for cocaine is essential for control of abuse of this drug in racing horses.
UR - http://www.scopus.com/inward/record.url?scp=0024537178&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024537178&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0024537178
VL - 10
SP - 1
EP - 14
JO - Research Communications in Substances of Abuse
JF - Research Communications in Substances of Abuse
SN - 0193-0818
IS - 1
ER -