Immune responses in pregnant cattle and bovine fetuses following experimental infection with neospora caninum

A. Andrianarivo, B. Barr, Mark L Anderson, Joan D Rowe, A. Packham, K. Sverlow, Patricia A Conrad

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

Humoral and cell-mediated immune (CMI) responses [i.e. proliferative responses and gamma interferon (IFN-γ) production], were elicited in five cows infected between 159 and 169 days of gestation by a combined intravenous-intramuscular inoculation of Neospora caninum tachyzoites. Analysis of antigen-specific immunoglobulin (IgG) subclasses revealed a predominant IgG2 response in two cows, a mixed IgG1-IgG2 response in two other cows and a predominant IgG1 response in one cow. No correlation was round between IgG2 titers and IFN-γ levels. CD4+ T cells were responsible for the CMI responses in peripheral blood mononuclear cells from three infected cows. All five fetuses removed from infected dams at week 9 post-infection (219-231 days of gestation) mounted strong Neospora-specific humoral responses and had a predominant IgG1 response, regardless of their ability to produce IFN-γ. However, CMI responses were highly variable between fetuses. These data indicate the complexity of the immune mechanisms associated with Neospora infection in both the dams and their fetuses.

Original languageEnglish (US)
Pages (from-to)817-825
Number of pages9
JournalParasitology Research
Volume87
Issue number10
DOIs
StatePublished - 2001

ASJC Scopus subject areas

  • Parasitology

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