Immune modulation by fish kinetoplastid parasites: A role for nitric oxide

Jeroen Saeij, W. B. Van Muiswinkel, A. Groeneveld, Geert F. Wiegertjes

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Trypanoplasma borreli and Trypanosoma carassii are kinetoplastid parasites infecting cyprinid fish. We investigated the role of nitric oxide (NO) in immune modulation during T. borreli and T. carassii infection of carp. Phagocytic cells from different organs produced NO and serum nitrate levels increased, demonstrating that T. borreli activates NO production in vivo. In contrast, T. carassii did not induce NO production in vivo and inhibited LPS-induced NO production in vitro. Production of NO was detrimental to the host as T. borreli-infected carp treated with the inducible NO synthase inhibitor aminoguanidine had a higher survival than infected control carp. This detrimental effect can be explained (in part) by the toxicity of NO to cells in vitro as NO inhibited the proliferative response of blood and spleen leukocytes. Head-kidney phagocytes were resistant to the immunosuppressive effects of NO in vitro. The NO-inducing activity of T. borreli may be an adaptation developed to ensure survival and immune evasion in the fish host. Apparently, T. carassii has adopted another strategy by deactivating specific functions of phagocytes. Both strategies may ensure long-term survival of the parasite.

Original languageEnglish (US)
Pages (from-to)77-86
Number of pages10
JournalParasitology
Volume124
Issue number1
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

nitric oxide
Nitric Oxide
Fishes
Parasites
parasites
fish
Carps
phagocytes
Phagocytes
carp
Head Kidney
immune evasion
Trypanosoma
Immune Evasion
immunosuppressive agents
Nitric Oxide Synthase Type II
Immunosuppressive Agents
blood serum
Nitrates
leukocytes

Keywords

  • Fish
  • Immunosuppression
  • Nitric oxide
  • Parasite
  • Trypanoplasma
  • Trypanosoma

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Cite this

Immune modulation by fish kinetoplastid parasites : A role for nitric oxide. / Saeij, Jeroen; Van Muiswinkel, W. B.; Groeneveld, A.; Wiegertjes, Geert F.

In: Parasitology, Vol. 124, No. 1, 2002, p. 77-86.

Research output: Contribution to journalArticle

Saeij, J, Van Muiswinkel, WB, Groeneveld, A & Wiegertjes, GF 2002, 'Immune modulation by fish kinetoplastid parasites: A role for nitric oxide', Parasitology, vol. 124, no. 1, pp. 77-86. https://doi.org/10.1017/S0031182001008915
Saeij, Jeroen ; Van Muiswinkel, W. B. ; Groeneveld, A. ; Wiegertjes, Geert F. / Immune modulation by fish kinetoplastid parasites : A role for nitric oxide. In: Parasitology. 2002 ; Vol. 124, No. 1. pp. 77-86.
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