Immobilization of the epoxide hydrolase from Aspergillus niger

S. Karboune, L. Amourache, H. Nellaiah, C. Morisseau, J. Baratti

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Three methods for the immobilization of the epoxide hydrolase from the fungus Aspergillus niger were tested. The highest immobilization yield (90%) and retention of activity (65%) were obtained by adsorption onto DEAE-cellulose compared to adsorption onto hydrophobic porous polypropylene and covalent linkage using Eupergit resin. The enzymatic properties of the immobilized enzyme were similar to those of the free enzyme with respect to the effect of temperature and pH on both activity and stability as well as the effect of solvent (DMF) on activity. The kinetic parameters were affected leading to lower KM(app) and higher Vm(app).

Original languageEnglish (US)
Pages (from-to)1633-1639
Number of pages7
JournalBiotechnology Letters
Volume23
Issue number19
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Epoxide Hydrolases
Hydrolases
Aspergillus niger
Aspergillus
Application programs
Immobilization
Adsorption
Enzymes
DEAE-Cellulose
Immobilized Enzymes
Polypropylenes
Fungi
Kinetic parameters
Cellulose
Resins
Temperature

Keywords

  • Aspergillus niger
  • Diffusional limitations
  • Epoxide hydrolase
  • Immobilization

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Karboune, S., Amourache, L., Nellaiah, H., Morisseau, C., & Baratti, J. (2001). Immobilization of the epoxide hydrolase from Aspergillus niger. Biotechnology Letters, 23(19), 1633-1639. https://doi.org/10.1023/A:1011940802411

Immobilization of the epoxide hydrolase from Aspergillus niger. / Karboune, S.; Amourache, L.; Nellaiah, H.; Morisseau, C.; Baratti, J.

In: Biotechnology Letters, Vol. 23, No. 19, 2001, p. 1633-1639.

Research output: Contribution to journalArticle

Karboune, S, Amourache, L, Nellaiah, H, Morisseau, C & Baratti, J 2001, 'Immobilization of the epoxide hydrolase from Aspergillus niger', Biotechnology Letters, vol. 23, no. 19, pp. 1633-1639. https://doi.org/10.1023/A:1011940802411
Karboune S, Amourache L, Nellaiah H, Morisseau C, Baratti J. Immobilization of the epoxide hydrolase from Aspergillus niger. Biotechnology Letters. 2001;23(19):1633-1639. https://doi.org/10.1023/A:1011940802411
Karboune, S. ; Amourache, L. ; Nellaiah, H. ; Morisseau, C. ; Baratti, J. / Immobilization of the epoxide hydrolase from Aspergillus niger. In: Biotechnology Letters. 2001 ; Vol. 23, No. 19. pp. 1633-1639.
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