Immobilization of the epoxide hydrolase from Aspergillus niger

S. Karboune, L. Amourache, H. Nellaiah, C. Morisseau, J. Baratti

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Three methods for the immobilization of the epoxide hydrolase from the fungus Aspergillus niger were tested. The highest immobilization yield (90%) and retention of activity (65%) were obtained by adsorption onto DEAE-cellulose compared to adsorption onto hydrophobic porous polypropylene and covalent linkage using Eupergit resin. The enzymatic properties of the immobilized enzyme were similar to those of the free enzyme with respect to the effect of temperature and pH on both activity and stability as well as the effect of solvent (DMF) on activity. The kinetic parameters were affected leading to lower KM(app) and higher Vm(app).

Original languageEnglish (US)
Pages (from-to)1633-1639
Number of pages7
JournalBiotechnology Letters
Volume23
Issue number19
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Aspergillus niger
  • Diffusional limitations
  • Epoxide hydrolase
  • Immobilization

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Fingerprint Dive into the research topics of 'Immobilization of the epoxide hydrolase from Aspergillus niger'. Together they form a unique fingerprint.

  • Cite this

    Karboune, S., Amourache, L., Nellaiah, H., Morisseau, C., & Baratti, J. (2001). Immobilization of the epoxide hydrolase from Aspergillus niger. Biotechnology Letters, 23(19), 1633-1639. https://doi.org/10.1023/A:1011940802411