Imbalance of excitatory/inhibitory synaptic protein expression in iPSC-derived neurons from FOXG1 patients and in foxg1 mice

Tommaso Patriarchi, Sonia Amabile, Elisa Frullanti, Elisa Landucci, Caterina Lo Rizzo, Francesca Ariani, Mario Costa, Francesco Olimpico, Johannes W Hell, Flora M. Vaccarino, Alessandra Renieri, Ilaria Meloni

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19 Citations (Scopus)

Abstract

Rett syndrome (RTT) is a severe neurodevelopmental disorder associated with mutations in either MECP2, CDKL5 or FOXG1. The precise molecular mechanisms that lead to the pathogenesis of RTT have yet to be elucidated. We recently reported that expression of GluD1 (orphan glutamate receptor δ-1 subunit) is increased in iPSC-derived neurons obtained from patients with mutations in either MECP2 or CDKL5. GluD1 controls synaptic differentiation and shifts the balance between excitatory and inhibitory synapses toward the latter. Thus, an increase in GluD1 might be a critical factor in the etiology of RTT by affecting the excitatory/inhibitory balance in the developing brain. To test this hypothesis, we generated iPSC-derived neurons from FOXG1 patients. We analyzed mRNA and protein levels of GluD1 together with key markers of excitatory and inhibitory synapses in these iPSC-derived neurons and in Foxg1 mouse fetal (E11.5) and adult (P70) brains. We found strong correlation between iPSC-derived neurons and fetal mouse brains, where GluD1 and inhibitory synaptic markers (GAD67 and GABA AR-1) were increased, whereas the levels of a number of excitatory synaptic markers (VGLUT1, GluA1, GluN1 and PSD-95) were decreased. In adult mice, GluD1 was decreased along with all GABAergic and glutamatergic markers. Our findings further the understanding of the etiology of RTT by introducing a new pathological event occurring in the brain of FOXG1 patients during embryonic development and its time-dependent shift toward a general decrease in brain synapses.

Original languageEnglish (US)
Pages (from-to)871-880
Number of pages10
JournalEuropean Journal of Human Genetics
Volume24
Issue number6
DOIs
StatePublished - Jun 1 2016

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Rett Syndrome
Neurons
Synapses
Brain
Proteins
Mutation
Orphaned Children
Glutamate Receptors
gamma-Aminobutyric Acid
Embryonic Development
Messenger RNA

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Imbalance of excitatory/inhibitory synaptic protein expression in iPSC-derived neurons from FOXG1 patients and in foxg1 mice. / Patriarchi, Tommaso; Amabile, Sonia; Frullanti, Elisa; Landucci, Elisa; Lo Rizzo, Caterina; Ariani, Francesca; Costa, Mario; Olimpico, Francesco; Hell, Johannes W; Vaccarino, Flora M.; Renieri, Alessandra; Meloni, Ilaria.

In: European Journal of Human Genetics, Vol. 24, No. 6, 01.06.2016, p. 871-880.

Research output: Contribution to journalArticle

Patriarchi, T, Amabile, S, Frullanti, E, Landucci, E, Lo Rizzo, C, Ariani, F, Costa, M, Olimpico, F, Hell, JW, Vaccarino, FM, Renieri, A & Meloni, I 2016, 'Imbalance of excitatory/inhibitory synaptic protein expression in iPSC-derived neurons from FOXG1 patients and in foxg1 mice', European Journal of Human Genetics, vol. 24, no. 6, pp. 871-880. https://doi.org/10.1038/ejhg.2015.216
Patriarchi, Tommaso ; Amabile, Sonia ; Frullanti, Elisa ; Landucci, Elisa ; Lo Rizzo, Caterina ; Ariani, Francesca ; Costa, Mario ; Olimpico, Francesco ; Hell, Johannes W ; Vaccarino, Flora M. ; Renieri, Alessandra ; Meloni, Ilaria. / Imbalance of excitatory/inhibitory synaptic protein expression in iPSC-derived neurons from FOXG1 patients and in foxg1 mice. In: European Journal of Human Genetics. 2016 ; Vol. 24, No. 6. pp. 871-880.
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AU - Amabile, Sonia

AU - Frullanti, Elisa

AU - Landucci, Elisa

AU - Lo Rizzo, Caterina

AU - Ariani, Francesca

AU - Costa, Mario

AU - Olimpico, Francesco

AU - Hell, Johannes W

AU - Vaccarino, Flora M.

AU - Renieri, Alessandra

AU - Meloni, Ilaria

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