TY - JOUR
T1 - Imaging voltage and brain chemistry with genetically encoded sensors and modulators
AU - Pal, Akash
AU - Tian, Lin
PY - 2020/8
Y1 - 2020/8
N2 - Neurons and glia are functionally organized into circuits and higher-order structures that allow the precise information processing required for complex behaviors. To better understand the structure and function of the brain, we must understand synaptic connectivity, action potential generation and propagation, as well as well-orchestrated molecular signaling. Recently, dramatically improved sensors for voltage, intracellular calcium, and neurotransmitters/modulators, combined with advanced microscopy provide new opportunities for in vivo dissection of cellular and circuit activity in awake, behaving animals. This review focuses on the current trends in genetically encoded sensors for molecules and cellular events and their potential applicability to the study of nervous system in health and disease.
AB - Neurons and glia are functionally organized into circuits and higher-order structures that allow the precise information processing required for complex behaviors. To better understand the structure and function of the brain, we must understand synaptic connectivity, action potential generation and propagation, as well as well-orchestrated molecular signaling. Recently, dramatically improved sensors for voltage, intracellular calcium, and neurotransmitters/modulators, combined with advanced microscopy provide new opportunities for in vivo dissection of cellular and circuit activity in awake, behaving animals. This review focuses on the current trends in genetically encoded sensors for molecules and cellular events and their potential applicability to the study of nervous system in health and disease.
KW - Bioluminescence
KW - GECI
KW - Genetically encoded sensors
KW - GEVI
KW - GEZI
UR - http://www.scopus.com/inward/record.url?scp=85089506229&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85089506229&partnerID=8YFLogxK
U2 - 10.1016/j.cbpa.2020.07.006
DO - 10.1016/j.cbpa.2020.07.006
M3 - Review article
C2 - 32823064
AN - SCOPUS:85089506229
VL - 57
SP - 166
EP - 176
JO - Current Opinion in Chemical Biology
JF - Current Opinion in Chemical Biology
SN - 1367-5931
ER -