Imaging transgene expression with radionuclide imaging technologies

S. S. Gambhir, H. R. Herschman, Simon R Cherry, J. R. Barrio, N. Satyamurthy, T. Toyokuni, M. E. Phelps, S. M. Larson, J. Balatoni, R. Finn, M. Sadelain, J. Tjuvajev, R. Blasberg

Research output: Contribution to journalArticle

314 Citations (Scopus)

Abstract

A variety of imaging technologies are being investigated as tools for studying gene expression in living subjects. Noninvasive, repetitive and quantitative imaging of gene expression will help both to facilitate human gene therapy trials and to allow for the study of animal models of molecular and cellular therapy. Radionuclide approaches using single photon emission computed tomography (SPECT) and positron emission tomography (PET) are the most mature of the current imaging technologies and offer many advantages for imaging gene expression compared to optical and magnetic resonance imaging (MRI)-based approaches. These advantages include relatively high sensitivity, full quantitative capability (for PET), and the ability to extend small animal assays directly into clinical human applications. We describe a PET scanner (microPET) designed specifically for studies of small animals. We review 'marker/reporter gene' imaging approaches using the herpes simplex type 1 virus thymidine kinase (HSV1-tk) and the dopamine type 2 receptor (D2R) genes. We describe and contrast several radiolabeled probes that can be used with the HSV1-tk reporter gene both for SPECT and for PET imaging. We also describe the advantages/disadvantages of each of the assays developed and discuss future animal and human applications.

Original languageEnglish (US)
Pages (from-to)118-138
Number of pages21
JournalNeoplasia
Volume2
Issue number1-2
StatePublished - Jan 2000

Fingerprint

Transgenes
Radionuclide Imaging
Positron-Emission Tomography
Technology
Single-Photon Emission-Computed Tomography
Reporter Genes
Gene Expression
Thymidine Kinase
Dopamine Receptors
Human Herpesvirus 1
Radioisotopes
Genetic Therapy
Animal Models
Magnetic Resonance Imaging
Genes
Therapeutics

Keywords

  • Dopamine 2 receptor
  • Gene expression
  • Herpes simplex virus
  • Imaging
  • Marker gene
  • MicroPET
  • PET
  • Reporter gene
  • SPECT
  • Thymidine kinase

ASJC Scopus subject areas

  • Cancer Research

Cite this

Gambhir, S. S., Herschman, H. R., Cherry, S. R., Barrio, J. R., Satyamurthy, N., Toyokuni, T., ... Blasberg, R. (2000). Imaging transgene expression with radionuclide imaging technologies. Neoplasia, 2(1-2), 118-138.

Imaging transgene expression with radionuclide imaging technologies. / Gambhir, S. S.; Herschman, H. R.; Cherry, Simon R; Barrio, J. R.; Satyamurthy, N.; Toyokuni, T.; Phelps, M. E.; Larson, S. M.; Balatoni, J.; Finn, R.; Sadelain, M.; Tjuvajev, J.; Blasberg, R.

In: Neoplasia, Vol. 2, No. 1-2, 01.2000, p. 118-138.

Research output: Contribution to journalArticle

Gambhir, SS, Herschman, HR, Cherry, SR, Barrio, JR, Satyamurthy, N, Toyokuni, T, Phelps, ME, Larson, SM, Balatoni, J, Finn, R, Sadelain, M, Tjuvajev, J & Blasberg, R 2000, 'Imaging transgene expression with radionuclide imaging technologies', Neoplasia, vol. 2, no. 1-2, pp. 118-138.
Gambhir SS, Herschman HR, Cherry SR, Barrio JR, Satyamurthy N, Toyokuni T et al. Imaging transgene expression with radionuclide imaging technologies. Neoplasia. 2000 Jan;2(1-2):118-138.
Gambhir, S. S. ; Herschman, H. R. ; Cherry, Simon R ; Barrio, J. R. ; Satyamurthy, N. ; Toyokuni, T. ; Phelps, M. E. ; Larson, S. M. ; Balatoni, J. ; Finn, R. ; Sadelain, M. ; Tjuvajev, J. ; Blasberg, R. / Imaging transgene expression with radionuclide imaging technologies. In: Neoplasia. 2000 ; Vol. 2, No. 1-2. pp. 118-138.
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