Imaging sites of receptor dephosphorylation by PTP1B on the surface of the endoplasmic reticulum

Fawaz Haj, Peter J. Verveer, Anthony Squire, Benjamin G. Neel, Philippe I H Bastiaens

Research output: Contribution to journalArticle

342 Citations (Scopus)

Abstract

When bound by extracellular ligands, receptor tyrosine kinases (RTKs) on the cell surface transmit critical signals to the cell interior. Although signal termination is less well understood, protein tyrosine 1B-(PTP1B) is implicated in the dephosphorylation and inactivation of several RTKs. However, PTP1B resides on the cytoplasmic surface of the endoplasmic reticulum (ER), so how and when it accesses RTKs has been unclear. Using fluorescence resonance energy transfer (FRET) methods, we monitored interactions between the epidermal- and platelet-derived growth factor receptors and PTP1B. PTP1B-catalyzed dephosphorylation required endocytosis of the receptors and occurred at specific sites on the surface of the ER. Most of the RTKs activated at the cell surface showed interaction with PTP1B after internalization, establishing that RTK activation and inactivation are spatially and temporally partitioned within cells.

Original languageEnglish (US)
Pages (from-to)1708-1711
Number of pages4
JournalScience
Volume295
Issue number5560
DOIs
StatePublished - Mar 1 2002
Externally publishedYes

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Receptor Protein-Tyrosine Kinases
Endoplasmic Reticulum
Platelet-Derived Growth Factor Receptors
Fluorescence Resonance Energy Transfer
Endocytosis
Tyrosine
Ligands
Proteins

ASJC Scopus subject areas

  • General

Cite this

Imaging sites of receptor dephosphorylation by PTP1B on the surface of the endoplasmic reticulum. / Haj, Fawaz; Verveer, Peter J.; Squire, Anthony; Neel, Benjamin G.; Bastiaens, Philippe I H.

In: Science, Vol. 295, No. 5560, 01.03.2002, p. 1708-1711.

Research output: Contribution to journalArticle

Haj, F, Verveer, PJ, Squire, A, Neel, BG & Bastiaens, PIH 2002, 'Imaging sites of receptor dephosphorylation by PTP1B on the surface of the endoplasmic reticulum', Science, vol. 295, no. 5560, pp. 1708-1711. https://doi.org/10.1126/science.1067566
Haj, Fawaz ; Verveer, Peter J. ; Squire, Anthony ; Neel, Benjamin G. ; Bastiaens, Philippe I H. / Imaging sites of receptor dephosphorylation by PTP1B on the surface of the endoplasmic reticulum. In: Science. 2002 ; Vol. 295, No. 5560. pp. 1708-1711.
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