Imaging retinal nerve fiber bundles at ultrahigh speed and ultrahigh resolution using OCT with adaptive optics

Omer Pars Kocaoglu, Barry Cense, Qiang Wang, Jeremy Bruestle, Jason Besecker, Weihua Gao, Ravi Jonnal, Donald T. Miller

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Ultrahigh speed line scan detectors based on CMOS technology have been recently demonstrated in ultrahigh resolution spectral-domain optical coherence tomography (UHR-SD-OCT) for retinal imaging. While successful, fundamental tradeoffs exist been image acquisition time, image sampling density, and sensitivity, all of which impact the extent of motion artifacts, visualization of fine spatial detail, and detection of faint reflections. Here we investigate these tradeoffs for imaging retinal nerve fiber bundles (RNFBs) using UHR-SD-OCT with adaptive optics (AO). Volume scans of 3°x3° and 1.5°x1.5° were acquired at retinal locations of 3° nasal and 6° superior to the fovea on a healthy subject. Dynamic AO compensation across a 6 mm pupil provided near-diffraction-limited performance. The acquisition rates were 22.5k lines/s and 125k lines/s with A-lines spaced at 0.9 μm and 1.8 μm and B-scans at 1.8 μm and 9 μm. Focus was optimized for visualizing the retinal nerve fiber bundles (RNFBs). En face projection and cross-sectional views of the RNFBs were extracted from the volumes and compared to images acquired with established conventional CCD-based line-scan camera. The projection view was found highly sensitive to eye motion artifacts, yet could only be partially compensated with coarser sampling, since fine sampling was necessary to observe the microscopic features in the RNFBs. For the cross-sectional view, speckle noise rather than eye motion artifacts limited bundle clarity. The highest B-scan density (1.8 μm spacing) coupled with B-scan averaging proved the best combination. Regardless of view, the higher line rate provided better RNFB clarity.

Original languageEnglish (US)
Title of host publicationOphthalmic Technologies XX
Volume7550
DOIs
StatePublished - Dec 1 2010
Externally publishedYes
EventOphthalmic Technologies XX - San Francisco, CA, United States
Duration: Jan 23 2010Jan 25 2010

Other

OtherOphthalmic Technologies XX
CountryUnited States
CitySan Francisco, CA
Period1/23/101/25/10

Fingerprint

nerve fibers
Adaptive optics
Optical resolving power
adaptive optics
Nerve Fibers
bundles
Imaging techniques
Fibers
Artifacts
Spectral resolution
Optical tomography
Optical Coherence Tomography
artifacts
clarity
sampling
tradeoffs
spectral resolution
Image sampling
acquisition
Sampling

Keywords

  • Adaptive optics
  • Optical coherence tomography
  • Retinal nerve fiber bundles

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Kocaoglu, O. P., Cense, B., Wang, Q., Bruestle, J., Besecker, J., Gao, W., ... Miller, D. T. (2010). Imaging retinal nerve fiber bundles at ultrahigh speed and ultrahigh resolution using OCT with adaptive optics. In Ophthalmic Technologies XX (Vol. 7550). [755010] https://doi.org/10.1117/12.846585

Imaging retinal nerve fiber bundles at ultrahigh speed and ultrahigh resolution using OCT with adaptive optics. / Kocaoglu, Omer Pars; Cense, Barry; Wang, Qiang; Bruestle, Jeremy; Besecker, Jason; Gao, Weihua; Jonnal, Ravi; Miller, Donald T.

Ophthalmic Technologies XX. Vol. 7550 2010. 755010.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kocaoglu, OP, Cense, B, Wang, Q, Bruestle, J, Besecker, J, Gao, W, Jonnal, R & Miller, DT 2010, Imaging retinal nerve fiber bundles at ultrahigh speed and ultrahigh resolution using OCT with adaptive optics. in Ophthalmic Technologies XX. vol. 7550, 755010, Ophthalmic Technologies XX, San Francisco, CA, United States, 1/23/10. https://doi.org/10.1117/12.846585
Kocaoglu OP, Cense B, Wang Q, Bruestle J, Besecker J, Gao W et al. Imaging retinal nerve fiber bundles at ultrahigh speed and ultrahigh resolution using OCT with adaptive optics. In Ophthalmic Technologies XX. Vol. 7550. 2010. 755010 https://doi.org/10.1117/12.846585
Kocaoglu, Omer Pars ; Cense, Barry ; Wang, Qiang ; Bruestle, Jeremy ; Besecker, Jason ; Gao, Weihua ; Jonnal, Ravi ; Miller, Donald T. / Imaging retinal nerve fiber bundles at ultrahigh speed and ultrahigh resolution using OCT with adaptive optics. Ophthalmic Technologies XX. Vol. 7550 2010.
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