Imaging of tissue microstructures using a multimodal microscope design

Stavros G. Demos; Chad A. Lieber; Bevin Lin; Rajendra Ramsamooj

doi:10.1109/JSTQE.2005.857385

Imaging of tissue microstructures using a multimodal microscope design

Stavros G. Demos, Chad A. Lieber, Bevin Lin, Rajendra Ramsamooj

Research output: Contribution to journal › Article

15 Scopus citations

Abstract

We investigate a microscope design that offers high signal sensitivity and hyperspectral imaging capabilities and allows for implementation of various optical imaging approaches while its operational complexity is minimized. This system uses long working distance microscope objectives that enable for offaxis illumination of the tissue, thereby allowing for excitation at any optical wavelength and nearly eliminating spectral noise from the optical elements. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissue microstructures using autofluorescence and light scattering imaging methods.

Original language	English (US)
Pages (from-to)	752-758
Number of pages	7
Journal	IEEE Journal on Selected Topics in Quantum Electronics
Volume	11
Issue number	4
DOIs	https://doi.org/10.1109/JSTQE.2005.857385
State	Published - Jul 2005

Keywords

Fluorescence microscopy
Medical and biological imaging
Microscopy
Tissue diagnostics

ASJC Scopus subject areas

Electrical and Electronic Engineering
Atomic and Molecular Physics, and Optics

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Demos, S. G., Lieber, C. A., Lin, B., & Ramsamooj, R. (2005). Imaging of tissue microstructures using a multimodal microscope design. IEEE Journal on Selected Topics in Quantum Electronics, 11(4), 752-758. https://doi.org/10.1109/JSTQE.2005.857385

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