Abstract
We investigate a microscope design that offers high signal sensitivity and hyperspectral imaging capabilities and allows for implementation of various optical imaging approaches while its operational complexity is minimized. This system uses long working distance microscope objectives that enable for offaxis illumination of the tissue, thereby allowing for excitation at any optical wavelength and nearly eliminating spectral noise from the optical elements. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissue microstructures using autofluorescence and light scattering imaging methods.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 752-758 |
| Number of pages | 7 |
| Journal | IEEE Journal on Selected Topics in Quantum Electronics |
| Volume | 11 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jul 2005 |
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Keywords
- Fluorescence microscopy
- Medical and biological imaging
- Microscopy
- Tissue diagnostics
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Atomic and Molecular Physics, and Optics
Cite this
Imaging of tissue microstructures using a multimodal microscope design. / Demos, Stavros G.; Lieber, Chad A.; Lin, Bevin; Ramsamooj, Rajendra.
In: IEEE Journal on Selected Topics in Quantum Electronics, Vol. 11, No. 4, 07.2005, p. 752-758.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Imaging of tissue microstructures using a multimodal microscope design
AU - Demos, Stavros G.
AU - Lieber, Chad A.
AU - Lin, Bevin
AU - Ramsamooj, Rajendra
PY - 2005/7
Y1 - 2005/7
N2 - We investigate a microscope design that offers high signal sensitivity and hyperspectral imaging capabilities and allows for implementation of various optical imaging approaches while its operational complexity is minimized. This system uses long working distance microscope objectives that enable for offaxis illumination of the tissue, thereby allowing for excitation at any optical wavelength and nearly eliminating spectral noise from the optical elements. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissue microstructures using autofluorescence and light scattering imaging methods.
AB - We investigate a microscope design that offers high signal sensitivity and hyperspectral imaging capabilities and allows for implementation of various optical imaging approaches while its operational complexity is minimized. This system uses long working distance microscope objectives that enable for offaxis illumination of the tissue, thereby allowing for excitation at any optical wavelength and nearly eliminating spectral noise from the optical elements. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissue microstructures using autofluorescence and light scattering imaging methods.
KW - Fluorescence microscopy
KW - Medical and biological imaging
KW - Microscopy
KW - Tissue diagnostics
UR - http://www.scopus.com/inward/record.url?scp=29144438149&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=29144438149&partnerID=8YFLogxK
U2 - 10.1109/JSTQE.2005.857385
DO - 10.1109/JSTQE.2005.857385
M3 - Article
VL - 11
SP - 752
EP - 758
JO - IEEE Journal of Selected Topics in Quantum Electronics
JF - IEEE Journal of Selected Topics in Quantum Electronics
SN - 1077-260X
IS - 4
ER -