Abstract
We investigate a microscope design that offers high signal sensitivity and hyperspectral imaging capabilities and allows for implementation of various optical imaging approaches while its operational complexity is minimized. This system uses long working distance microscope objectives that enable for offaxis illumination of the tissue, thereby allowing for excitation at any optical wavelength and nearly eliminating spectral noise from the optical elements. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissue microstructures using autofluorescence and light scattering imaging methods.
Original language | English (US) |
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Pages (from-to) | 752-758 |
Number of pages | 7 |
Journal | IEEE Journal on Selected Topics in Quantum Electronics |
Volume | 11 |
Issue number | 4 |
DOIs | |
State | Published - Jul 2005 |
Keywords
- Fluorescence microscopy
- Medical and biological imaging
- Microscopy
- Tissue diagnostics
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Atomic and Molecular Physics, and Optics