Imaging of angiogenesis using Cadence™ contrast pulse sequencing and targeted contrast agents

Susanne M. Stieger, Paul A. Dayton, Mark A. Borden, Charles F. Caskey, Stephen M Griffey, Erik R Wisner, Katherine W. Ferrara

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Objectives: Low-power multipulse contrast ultrasound imaging provides a promising tool to quantify angiogenesis noninvasively when used with contrast agents targeted to vascular markers expressed by the angiogenic endothelium. Targeted ultrasound contrast agents, with a diameter on the order of micrometers, cannot extravasate and therefore are targeted solely to receptors expressed by the vascular endothelium. The aim of this study was to evaluate the potential of a low-power multipulse imaging sequence, Cadence™ contrast pulse sequencing (CPS), combined with targeted contrast agents to quantify angiogenesis. Material and Methods: Targeted microbubbles were prepared by conjugating echistatin via biotin-avidin linkage to the surface of a phospholipid microbubble shell. The density of echistatin present on the shell was confirmed with flow-cytometry and quantified by total fluorescence. The binding of targeted microbubbles was evaluated in vitro by quantifying the adherence of targeted microbubbles to rat aortic endothelial cells, compared with control (nontargeted) microbubbles. The circulation time and adherence of targeted microbubbles was evaluated in vivo in a Matrigel model in rats and compared with control microbubbles using CPS in addition to a destructive ultrasound pulse. Results: Using only the low-power CPS pulse, the echo intensity produced in the neovasculature of the Matrigel pellet was significantly greater with targeted microbubbles than with the control contrast agent (p < 0.001). Combining CPS with the destructive pulse, the processed image was significantly different in intensity (p < 0.001) and spatial extent between targeted and control agents (p < 0.001). When the morphology of the histological sample and ultrasound image correlated, the microvessel density count and the percentage of the circular area enhanced by ultrasound were correlated (p < 0.05). Conclusion: Low-power multipulse imaging in combination with targeted echistatin-bearing microbubbles facilitated a noninvasive, quantitative evaluation of early angiogenesis during real-time imaging. The addition of high-intensity destructive pulses facilitated estimation of the spatial extent of angiogenesis.

Original languageEnglish (US)
Pages (from-to)9-18
Number of pages10
JournalContrast Media and Molecular Imaging
Volume3
Issue number1
DOIs
StatePublished - Jan 2008

Fingerprint

Microbubbles
Contrast Media
Pulse
Avidin
Vascular Endothelium
Biotin
Microvessels
Endothelium
Blood Vessels
Ultrasonography
Phospholipids
Flow Cytometry
Endothelial Cells
Fluorescence

Keywords

  • Angiogenesis
  • CPS
  • Microbubbles
  • Molecular imaging
  • Ultrasound

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Imaging of angiogenesis using Cadence™ contrast pulse sequencing and targeted contrast agents. / Stieger, Susanne M.; Dayton, Paul A.; Borden, Mark A.; Caskey, Charles F.; Griffey, Stephen M; Wisner, Erik R; Ferrara, Katherine W.

In: Contrast Media and Molecular Imaging, Vol. 3, No. 1, 01.2008, p. 9-18.

Research output: Contribution to journalArticle

Stieger, Susanne M. ; Dayton, Paul A. ; Borden, Mark A. ; Caskey, Charles F. ; Griffey, Stephen M ; Wisner, Erik R ; Ferrara, Katherine W. / Imaging of angiogenesis using Cadence™ contrast pulse sequencing and targeted contrast agents. In: Contrast Media and Molecular Imaging. 2008 ; Vol. 3, No. 1. pp. 9-18.
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AU - Dayton, Paul A.

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AU - Griffey, Stephen M

AU - Wisner, Erik R

AU - Ferrara, Katherine W.

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