Abstract
Imaging microscopy with short pulse (200 ps) x-ray lasers offers the opportunity of high resolution three-dimensional imaging of specimens in an aqueous environment without the blurring effects associated with natural motion. As a first step toward this goal we have performed imaging experiments which clearly resolved 500 angstrom features on a gold test pattern. In addition, we have taken images of dried biological specimens as a basis for comparison in future wet specimen imaging. The results of these experiments are described. We also discuss some of the alignment problems involved in doing x-ray microscopy with low repetition rate systems where in situ focusing of the imaging optics is not practical as single shot exposures can alter the specimen.
| Original language | English (US) |
|---|---|
| Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
| Place of Publication | Bellingham, WA, United States |
| Publisher | Publ by Int Soc for Optical Engineering |
| Pages | 154-159 |
| Number of pages | 6 |
| Volume | 1741 |
| ISBN (Print) | 0819409146 |
| State | Published - 1993 |
| Externally published | Yes |
| Event | Soft X-Ray Microscopy - San Diego, CA, USA Duration: Jul 19 1992 → Jul 21 1992 |
Other
| Other | Soft X-Ray Microscopy |
|---|---|
| City | San Diego, CA, USA |
| Period | 7/19/92 → 7/21/92 |
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ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Condensed Matter Physics
Cite this
Imaging microscopy with x-ray lasers at LLNL. / Da Silva, Luiz B.; Trebes, James E.; Mrowka, Stan J.; MacGowan, Brian J.; Koch, Jeffrey A.; Matthews, Dennis L; Barbee, Troy W.; Balhorn, Rod; Gray, Joe W.; Anderson, Erik H.; Kern, D.; Attwood, David T.
Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1741 Bellingham, WA, United States : Publ by Int Soc for Optical Engineering, 1993. p. 154-159.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Imaging microscopy with x-ray lasers at LLNL
AU - Da Silva, Luiz B.
AU - Trebes, James E.
AU - Mrowka, Stan J.
AU - MacGowan, Brian J.
AU - Koch, Jeffrey A.
AU - Matthews, Dennis L
AU - Barbee, Troy W.
AU - Balhorn, Rod
AU - Gray, Joe W.
AU - Anderson, Erik H.
AU - Kern, D.
AU - Attwood, David T.
PY - 1993
Y1 - 1993
N2 - Imaging microscopy with short pulse (200 ps) x-ray lasers offers the opportunity of high resolution three-dimensional imaging of specimens in an aqueous environment without the blurring effects associated with natural motion. As a first step toward this goal we have performed imaging experiments which clearly resolved 500 angstrom features on a gold test pattern. In addition, we have taken images of dried biological specimens as a basis for comparison in future wet specimen imaging. The results of these experiments are described. We also discuss some of the alignment problems involved in doing x-ray microscopy with low repetition rate systems where in situ focusing of the imaging optics is not practical as single shot exposures can alter the specimen.
AB - Imaging microscopy with short pulse (200 ps) x-ray lasers offers the opportunity of high resolution three-dimensional imaging of specimens in an aqueous environment without the blurring effects associated with natural motion. As a first step toward this goal we have performed imaging experiments which clearly resolved 500 angstrom features on a gold test pattern. In addition, we have taken images of dried biological specimens as a basis for comparison in future wet specimen imaging. The results of these experiments are described. We also discuss some of the alignment problems involved in doing x-ray microscopy with low repetition rate systems where in situ focusing of the imaging optics is not practical as single shot exposures can alter the specimen.
UR - http://www.scopus.com/inward/record.url?scp=0027147085&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027147085&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:0027147085
SN - 0819409146
VL - 1741
SP - 154
EP - 159
BT - Proceedings of SPIE - The International Society for Optical Engineering
PB - Publ by Int Soc for Optical Engineering
CY - Bellingham, WA, United States
ER -