Abstract
Superimposed on the vast and complex synaptic network is a largely invisible set of chemical inputs, such as neurotransmitters and neuromodulators, that exert a profound influence on brain function across many structures and temporal scales. Thus, the determination of the spatiotemporal relationships between these chemical signals with synaptic resolution in the intact brain is essential to decipher the codes for transferring information across circuitry and systems. Recent advances in imaging technology have been employed to determine the extent of spatial and temporal neurotransmitter dynamics in the brain, especially glutamate, the most abundant excitatory neurotransmitter. Here, we discuss recent imaging approaches, particularly with a focus on the design and application of genetically encoded indicator iGluSnFR, in analyzing glutamate transients in vitro, ex vivo, and in vivo.
| Original language | English (US) |
|---|---|
| Title of host publication | Neuromethods |
| Publisher | Humana Press Inc. |
| Pages | 117-153 |
| Number of pages | 37 |
| Volume | 130 |
| DOIs | |
| State | Published - 2018 |
Publication series
| Name | Neuromethods |
|---|---|
| Volume | 130 |
| ISSN (Print) | 0893-2336 |
| ISSN (Electronic) | 1940-6045 |
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Keywords
- Fluorescent functional imaging
- Fluorescent sensor
- Genetically encoded indicators of neural activity
- Glutamate
- iGluSnFR
- Protein engineering
ASJC Scopus subject areas
- Neuroscience(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)
- Psychiatry and Mental health
Cite this
Imaging glutamate with genetically encoded fluorescent sensors. / Broussard, Gerard J.; Unger, Elizabeth K.; Liang, Ruqiang; McGrew, Brian P.; Tian, Lin.
Neuromethods. Vol. 130 Humana Press Inc., 2018. p. 117-153 (Neuromethods; Vol. 130).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Imaging glutamate with genetically encoded fluorescent sensors
AU - Broussard, Gerard J.
AU - Unger, Elizabeth K.
AU - Liang, Ruqiang
AU - McGrew, Brian P.
AU - Tian, Lin
PY - 2018
Y1 - 2018
N2 - Superimposed on the vast and complex synaptic network is a largely invisible set of chemical inputs, such as neurotransmitters and neuromodulators, that exert a profound influence on brain function across many structures and temporal scales. Thus, the determination of the spatiotemporal relationships between these chemical signals with synaptic resolution in the intact brain is essential to decipher the codes for transferring information across circuitry and systems. Recent advances in imaging technology have been employed to determine the extent of spatial and temporal neurotransmitter dynamics in the brain, especially glutamate, the most abundant excitatory neurotransmitter. Here, we discuss recent imaging approaches, particularly with a focus on the design and application of genetically encoded indicator iGluSnFR, in analyzing glutamate transients in vitro, ex vivo, and in vivo.
AB - Superimposed on the vast and complex synaptic network is a largely invisible set of chemical inputs, such as neurotransmitters and neuromodulators, that exert a profound influence on brain function across many structures and temporal scales. Thus, the determination of the spatiotemporal relationships between these chemical signals with synaptic resolution in the intact brain is essential to decipher the codes for transferring information across circuitry and systems. Recent advances in imaging technology have been employed to determine the extent of spatial and temporal neurotransmitter dynamics in the brain, especially glutamate, the most abundant excitatory neurotransmitter. Here, we discuss recent imaging approaches, particularly with a focus on the design and application of genetically encoded indicator iGluSnFR, in analyzing glutamate transients in vitro, ex vivo, and in vivo.
KW - Fluorescent functional imaging
KW - Fluorescent sensor
KW - Genetically encoded indicators of neural activity
KW - Glutamate
KW - iGluSnFR
KW - Protein engineering
UR - http://www.scopus.com/inward/record.url?scp=85030119480&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85030119480&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-7228-9_5
DO - 10.1007/978-1-4939-7228-9_5
M3 - Chapter
AN - SCOPUS:85030119480
VL - 130
T3 - Neuromethods
SP - 117
EP - 153
BT - Neuromethods
PB - Humana Press Inc.
ER -