Imaging glutamate with genetically encoded fluorescent sensors

Gerard J. Broussard, Elizabeth K. Unger, Ruqiang Liang, Brian P. McGrew, Lin Tian

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Superimposed on the vast and complex synaptic network is a largely invisible set of chemical inputs, such as neurotransmitters and neuromodulators, that exert a profound influence on brain function across many structures and temporal scales. Thus, the determination of the spatiotemporal relationships between these chemical signals with synaptic resolution in the intact brain is essential to decipher the codes for transferring information across circuitry and systems. Recent advances in imaging technology have been employed to determine the extent of spatial and temporal neurotransmitter dynamics in the brain, especially glutamate, the most abundant excitatory neurotransmitter. Here, we discuss recent imaging approaches, particularly with a focus on the design and application of genetically encoded indicator iGluSnFR, in analyzing glutamate transients in vitro, ex vivo, and in vivo.

Original languageEnglish (US)
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages117-153
Number of pages37
Volume130
DOIs
StatePublished - 2018

Publication series

NameNeuromethods
Volume130
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • Fluorescent functional imaging
  • Fluorescent sensor
  • Genetically encoded indicators of neural activity
  • Glutamate
  • iGluSnFR
  • Protein engineering

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

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