Imaging extra-thoracic airways and deposited particles in laboratory animals

Praveen K. Srirama, Chris D. Wallis, DongYoub Lee, Anthony S. Wexler

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Laboratory animals are widely used as models for exploring adverse and beneficial health effects caused by inhalation of suspended particles. Mapping particle deposition throughout the airways of laboratory animals is crucial since the location of particle deposition often determines subsequent clearance, transport and the health effects elicited. Such mapping has yet to be performed systematically due to lack of available methods. In this paper, a new method is presented for imaging both the nasal airways of laboratory animals and particles deposited in them. After inhalation of particles, the rat airways are frozen to lock the particles in place and cast at this temperature to color and support the lumen. Once the cast is cured, an imaging cryomicrotome sections and images the airways as well as the deposited fluorescent particles. Computer software reconstructs the airways and particle maps from these serial images. The method was validated by manually placing boluses of fluorescent particles in nasal airways of a rat and then imaging them to ensure that they were displayed in the correct locations. To demonstrate the method, rats inhaled 2.5μm particles in a nose-only exposure chamber. Images and reconstructions are presented of the nasal passage and deposited particles for one of these rats demonstrating the method's usefulness for locating inhaled and deposited therapeutic and toxic particles in the extra-thoracic airways of laboratory animals.

Original languageEnglish (US)
Pages (from-to)40-49
Number of pages10
JournalJournal of Aerosol Science
Volume45
DOIs
StatePublished - Mar 2012

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Animals
Imaging techniques
animal
Rats
Health
Poisons
laboratory
particle
Color
method
software
Temperature

Keywords

  • Air pollution
  • Cryomicrotome
  • Drug delivery
  • Fluorescent particles
  • Imaging
  • Nasal deposition

ASJC Scopus subject areas

  • Materials Science(all)
  • Environmental Chemistry
  • Pollution

Cite this

Imaging extra-thoracic airways and deposited particles in laboratory animals. / Srirama, Praveen K.; Wallis, Chris D.; Lee, DongYoub; Wexler, Anthony S.

In: Journal of Aerosol Science, Vol. 45, 03.2012, p. 40-49.

Research output: Contribution to journalArticle

Srirama, Praveen K. ; Wallis, Chris D. ; Lee, DongYoub ; Wexler, Anthony S. / Imaging extra-thoracic airways and deposited particles in laboratory animals. In: Journal of Aerosol Science. 2012 ; Vol. 45. pp. 40-49.
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