Imaging and differentiation of mouse embryo tissues by ToF-SIMS

Ligang Wu, Xiaochen Lu, Kristen S. Kulp, Mark G. Knize, Elena S F Berman, Erik J. Nelson, James S. Felton, Kuang Jen J Wu

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) equipped with a gold ion gun was used to image mouse embryo sections and differentiate tissue types (brain, spinal cord, skull, rib, heart and liver). Embryos were paraffin-embedded and then deparaffinized. The robustness and repeatability of the method was determined by analyzing ten tissue slices from three different embryos over a period of several weeks. Using principal component analysis (PCA) to reduce the spectral data generated by ToF-SIMS, histopathologically identified tissue types of the mouse embryos can be differentiated based on the characteristic differences in their mass spectra. These results demonstrate the ability of ToF-SIMS to determine subtle chemical differences even in fixed histological specimens.

Original languageEnglish (US)
Pages (from-to)137-145
Number of pages9
JournalInternational Journal of Mass Spectrometry
Volume260
Issue number2-3
DOIs
StatePublished - Feb 1 2007
Externally publishedYes

Fingerprint

embryos
Secondary ion mass spectrometry
secondary ion mass spectrometry
mice
Tissue
Imaging techniques
Ion sources
Gold
Liver
Paraffin
spinal cord
Principal component analysis
Paraffins
skull
Brain
paraffins
principal components analysis
liver
mass spectra
brain

Keywords

  • Image PCA
  • Mouse embryo
  • Paraffin-embedded
  • ToF-SIMS

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Spectroscopy

Cite this

Wu, L., Lu, X., Kulp, K. S., Knize, M. G., Berman, E. S. F., Nelson, E. J., ... Wu, K. J. J. (2007). Imaging and differentiation of mouse embryo tissues by ToF-SIMS. International Journal of Mass Spectrometry, 260(2-3), 137-145. https://doi.org/10.1016/j.ijms.2006.09.029

Imaging and differentiation of mouse embryo tissues by ToF-SIMS. / Wu, Ligang; Lu, Xiaochen; Kulp, Kristen S.; Knize, Mark G.; Berman, Elena S F; Nelson, Erik J.; Felton, James S.; Wu, Kuang Jen J.

In: International Journal of Mass Spectrometry, Vol. 260, No. 2-3, 01.02.2007, p. 137-145.

Research output: Contribution to journalArticle

Wu, L, Lu, X, Kulp, KS, Knize, MG, Berman, ESF, Nelson, EJ, Felton, JS & Wu, KJJ 2007, 'Imaging and differentiation of mouse embryo tissues by ToF-SIMS', International Journal of Mass Spectrometry, vol. 260, no. 2-3, pp. 137-145. https://doi.org/10.1016/j.ijms.2006.09.029
Wu, Ligang ; Lu, Xiaochen ; Kulp, Kristen S. ; Knize, Mark G. ; Berman, Elena S F ; Nelson, Erik J. ; Felton, James S. ; Wu, Kuang Jen J. / Imaging and differentiation of mouse embryo tissues by ToF-SIMS. In: International Journal of Mass Spectrometry. 2007 ; Vol. 260, No. 2-3. pp. 137-145.
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