Imaging and differentiation of mouse embryo tissues by ToF-SIMS

Ligang Wu; Xiaochen Lu; Kristen S. Kulp; Mark G. Knize; Elena S F Berman; Erik J. Nelson; James S. Felton; Kuang Jen J Wu

doi:10.1016/j.ijms.2006.09.029

Imaging and differentiation of mouse embryo tissues by ToF-SIMS

Ligang Wu, Xiaochen Lu, Kristen S. Kulp, Mark G. Knize, Elena S F Berman, Erik J. Nelson, James S. Felton, Kuang Jen J Wu

Research output: Contribution to journal › Article

37 Scopus citations

Abstract

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) equipped with a gold ion gun was used to image mouse embryo sections and differentiate tissue types (brain, spinal cord, skull, rib, heart and liver). Embryos were paraffin-embedded and then deparaffinized. The robustness and repeatability of the method was determined by analyzing ten tissue slices from three different embryos over a period of several weeks. Using principal component analysis (PCA) to reduce the spectral data generated by ToF-SIMS, histopathologically identified tissue types of the mouse embryos can be differentiated based on the characteristic differences in their mass spectra. These results demonstrate the ability of ToF-SIMS to determine subtle chemical differences even in fixed histological specimens.

Original language	English (US)
Pages (from-to)	137-145
Number of pages	9
Journal	International Journal of Mass Spectrometry
Volume	260
Issue number	2-3
DOIs	https://doi.org/10.1016/j.ijms.2006.09.029
State	Published - Feb 1 2007
Externally published	Yes

Keywords

Image PCA
Mouse embryo
Paraffin-embedded
ToF-SIMS

ASJC Scopus subject areas

Physical and Theoretical Chemistry
Spectroscopy

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Wu, L., Lu, X., Kulp, K. S., Knize, M. G., Berman, E. S. F., Nelson, E. J., Felton, J. S., & Wu, K. J. J. (2007). Imaging and differentiation of mouse embryo tissues by ToF-SIMS. International Journal of Mass Spectrometry, 260(2-3), 137-145. https://doi.org/10.1016/j.ijms.2006.09.029

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title = "Imaging and differentiation of mouse embryo tissues by ToF-SIMS",

abstract = "Time-of-flight secondary ion mass spectrometry (ToF-SIMS) equipped with a gold ion gun was used to image mouse embryo sections and differentiate tissue types (brain, spinal cord, skull, rib, heart and liver). Embryos were paraffin-embedded and then deparaffinized. The robustness and repeatability of the method was determined by analyzing ten tissue slices from three different embryos over a period of several weeks. Using principal component analysis (PCA) to reduce the spectral data generated by ToF-SIMS, histopathologically identified tissue types of the mouse embryos can be differentiated based on the characteristic differences in their mass spectra. These results demonstrate the ability of ToF-SIMS to determine subtle chemical differences even in fixed histological specimens.",

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author = "Ligang Wu and Xiaochen Lu and Kulp, {Kristen S.} and Knize, {Mark G.} and Berman, {Elena S F} and Nelson, {Erik J.} and Felton, {James S.} and Wu, {Kuang Jen J}",

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AU - Wu, Ligang

AU - Lu, Xiaochen

AU - Kulp, Kristen S.

AU - Knize, Mark G.

AU - Berman, Elena S F

AU - Nelson, Erik J.

AU - Felton, James S.

AU - Wu, Kuang Jen J

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AB - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) equipped with a gold ion gun was used to image mouse embryo sections and differentiate tissue types (brain, spinal cord, skull, rib, heart and liver). Embryos were paraffin-embedded and then deparaffinized. The robustness and repeatability of the method was determined by analyzing ten tissue slices from three different embryos over a period of several weeks. Using principal component analysis (PCA) to reduce the spectral data generated by ToF-SIMS, histopathologically identified tissue types of the mouse embryos can be differentiated based on the characteristic differences in their mass spectra. These results demonstrate the ability of ToF-SIMS to determine subtle chemical differences even in fixed histological specimens.

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