Abstract
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) equipped with a gold ion gun was used to image mouse embryo sections and differentiate tissue types (brain, spinal cord, skull, rib, heart and liver). Embryos were paraffin-embedded and then deparaffinized. The robustness and repeatability of the method was determined by analyzing ten tissue slices from three different embryos over a period of several weeks. Using principal component analysis (PCA) to reduce the spectral data generated by ToF-SIMS, histopathologically identified tissue types of the mouse embryos can be differentiated based on the characteristic differences in their mass spectra. These results demonstrate the ability of ToF-SIMS to determine subtle chemical differences even in fixed histological specimens.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 137-145 |
| Number of pages | 9 |
| Journal | International Journal of Mass Spectrometry |
| Volume | 260 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - Feb 1 2007 |
| Externally published | Yes |
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Keywords
- Image PCA
- Mouse embryo
- Paraffin-embedded
- ToF-SIMS
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Spectroscopy
Cite this
Imaging and differentiation of mouse embryo tissues by ToF-SIMS. / Wu, Ligang; Lu, Xiaochen; Kulp, Kristen S.; Knize, Mark G.; Berman, Elena S F; Nelson, Erik J.; Felton, James S.; Wu, Kuang Jen J.
In: International Journal of Mass Spectrometry, Vol. 260, No. 2-3, 01.02.2007, p. 137-145.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Imaging and differentiation of mouse embryo tissues by ToF-SIMS
AU - Wu, Ligang
AU - Lu, Xiaochen
AU - Kulp, Kristen S.
AU - Knize, Mark G.
AU - Berman, Elena S F
AU - Nelson, Erik J.
AU - Felton, James S.
AU - Wu, Kuang Jen J
PY - 2007/2/1
Y1 - 2007/2/1
N2 - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) equipped with a gold ion gun was used to image mouse embryo sections and differentiate tissue types (brain, spinal cord, skull, rib, heart and liver). Embryos were paraffin-embedded and then deparaffinized. The robustness and repeatability of the method was determined by analyzing ten tissue slices from three different embryos over a period of several weeks. Using principal component analysis (PCA) to reduce the spectral data generated by ToF-SIMS, histopathologically identified tissue types of the mouse embryos can be differentiated based on the characteristic differences in their mass spectra. These results demonstrate the ability of ToF-SIMS to determine subtle chemical differences even in fixed histological specimens.
AB - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) equipped with a gold ion gun was used to image mouse embryo sections and differentiate tissue types (brain, spinal cord, skull, rib, heart and liver). Embryos were paraffin-embedded and then deparaffinized. The robustness and repeatability of the method was determined by analyzing ten tissue slices from three different embryos over a period of several weeks. Using principal component analysis (PCA) to reduce the spectral data generated by ToF-SIMS, histopathologically identified tissue types of the mouse embryos can be differentiated based on the characteristic differences in their mass spectra. These results demonstrate the ability of ToF-SIMS to determine subtle chemical differences even in fixed histological specimens.
KW - Image PCA
KW - Mouse embryo
KW - Paraffin-embedded
KW - ToF-SIMS
UR - http://www.scopus.com/inward/record.url?scp=33845892186&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845892186&partnerID=8YFLogxK
U2 - 10.1016/j.ijms.2006.09.029
DO - 10.1016/j.ijms.2006.09.029
M3 - Article
AN - SCOPUS:33845892186
VL - 260
SP - 137
EP - 145
JO - International Journal of Mass Spectrometry
JF - International Journal of Mass Spectrometry
SN - 1387-3806
IS - 2-3
ER -