Abstract
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) equipped with a gold ion gun was used to image mouse embryo sections and differentiate tissue types (brain, spinal cord, skull, rib, heart and liver). Embryos were paraffin-embedded and then deparaffinized. The robustness and repeatability of the method was determined by analyzing ten tissue slices from three different embryos over a period of several weeks. Using principal component analysis (PCA) to reduce the spectral data generated by ToF-SIMS, histopathologically identified tissue types of the mouse embryos can be differentiated based on the characteristic differences in their mass spectra. These results demonstrate the ability of ToF-SIMS to determine subtle chemical differences even in fixed histological specimens.
Original language | English (US) |
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Pages (from-to) | 137-145 |
Number of pages | 9 |
Journal | International Journal of Mass Spectrometry |
Volume | 260 |
Issue number | 2-3 |
DOIs | |
State | Published - Feb 1 2007 |
Externally published | Yes |
Keywords
- Image PCA
- Mouse embryo
- Paraffin-embedded
- ToF-SIMS
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Spectroscopy