Ilheus Virus Isolation in the Pantanal, West-Central Brazil

Alex Pauvolid-Corrêa, Joan L. Kenney, Dinair Couto-Lima, Zilca M.S. Campos, Hermann G. Schatzmayr, Rita M.R. Nogueira, Aaron Brault, Nicholas Komar

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The wetlands of the Brazilian Pantanal host large concentrations of diverse wildlife species and hematophagous arthropods, conditions that favor the circulation of zoonotic arboviruses. A recent study from the Nhecolândia sub-region of Pantanal reported serological evidence of various flaviviruses, including West Nile virus and Ilheus virus (ILHV). According to the age of seropositive horses, at least three flaviviruses, including ILHV, circulated in the Brazilian Pantanal between 2005 and 2009. To extend this study, we collected 3,234 adult mosquitoes of 16 species during 2009 and 2010 in the same sub-region. Mosquito pool homogenates were assayed for infectious virus on C6/36 and Vero cell monolayers and also tested for flaviviral RNA by a group-specific real-time RT-PCR. One pool containing 50 non-engorged female specimens of Aedes scapularis tested positive for ILHV by culture and for ILHV RNA by real-time RT-PCR, indicating a minimum infection rate of 2.5 per 1000. Full-length genomic sequence exhibited 95% identity to the only full genome sequence available for ILHV. The present data confirm the circulation of ILHV in the Brazilian Pantanal.

Original languageEnglish (US)
Article numbere2318
JournalPLoS Neglected Tropical Diseases
Volume7
Issue number7
DOIs
StatePublished - Jan 1 2013
Externally publishedYes

Fingerprint

Brazil
Viruses
Flavivirus
Culicidae
Real-Time Polymerase Chain Reaction
Arboviruses
West Nile virus
Vero Cells
Arthropods
Wetlands
Aedes
RNA Viruses
Zoonoses
Horses
Genome
RNA
Infection

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Pauvolid-Corrêa, A., Kenney, J. L., Couto-Lima, D., Campos, Z. M. S., Schatzmayr, H. G., Nogueira, R. M. R., ... Komar, N. (2013). Ilheus Virus Isolation in the Pantanal, West-Central Brazil. PLoS Neglected Tropical Diseases, 7(7), [e2318]. https://doi.org/10.1371/journal.pntd.0002318

Ilheus Virus Isolation in the Pantanal, West-Central Brazil. / Pauvolid-Corrêa, Alex; Kenney, Joan L.; Couto-Lima, Dinair; Campos, Zilca M.S.; Schatzmayr, Hermann G.; Nogueira, Rita M.R.; Brault, Aaron; Komar, Nicholas.

In: PLoS Neglected Tropical Diseases, Vol. 7, No. 7, e2318, 01.01.2013.

Research output: Contribution to journalArticle

Pauvolid-Corrêa, A, Kenney, JL, Couto-Lima, D, Campos, ZMS, Schatzmayr, HG, Nogueira, RMR, Brault, A & Komar, N 2013, 'Ilheus Virus Isolation in the Pantanal, West-Central Brazil', PLoS Neglected Tropical Diseases, vol. 7, no. 7, e2318. https://doi.org/10.1371/journal.pntd.0002318
Pauvolid-Corrêa A, Kenney JL, Couto-Lima D, Campos ZMS, Schatzmayr HG, Nogueira RMR et al. Ilheus Virus Isolation in the Pantanal, West-Central Brazil. PLoS Neglected Tropical Diseases. 2013 Jan 1;7(7). e2318. https://doi.org/10.1371/journal.pntd.0002318
Pauvolid-Corrêa, Alex ; Kenney, Joan L. ; Couto-Lima, Dinair ; Campos, Zilca M.S. ; Schatzmayr, Hermann G. ; Nogueira, Rita M.R. ; Brault, Aaron ; Komar, Nicholas. / Ilheus Virus Isolation in the Pantanal, West-Central Brazil. In: PLoS Neglected Tropical Diseases. 2013 ; Vol. 7, No. 7.
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