Neutrophil (PMN) emigration at regions of inflammation involves chemotactic activation that leads to adhesion via β2-integrins binding to endothelial expressed ICAM-1. Flow cytometry was used to measure the kinetics of neutrophil adhesion to fluorescent latex microspheres (1 μm) derivatized with recombinant ICAM-1-Ig. The relationship between the dose of IL-8 simulation and the rate and extent of bead capture under fluid shear was examined. Onset of adhesion was detected at 50pM of IL-8, as few as 10 IL-8 receptors/PMN. The rate and extent of bead capture appeared to decrease nonlinearity with the site density of ICAM-1.
|Original language||English (US)|
|Journal||Annals of Biomedical Engineering|
|Issue number||SUPPL. 1|
|Publication status||Published - 2000|
ASJC Scopus subject areas
- Biomedical Engineering