IL-8 dose and ICAM-1 site density determine the dynamics of neutrophil adhesion under fluid shear

Aaron Lum, Scott Simon

Research output: Contribution to journalArticle

Abstract

Neutrophil (PMN) emigration at regions of inflammation involves chemotactic activation that leads to adhesion via β2-integrins binding to endothelial expressed ICAM-1. Flow cytometry was used to measure the kinetics of neutrophil adhesion to fluorescent latex microspheres (1 μm) derivatized with recombinant ICAM-1-Ig. The relationship between the dose of IL-8 simulation and the rate and extent of bead capture under fluid shear was examined. Onset of adhesion was detected at 50pM of IL-8, as few as 10 IL-8 receptors/PMN. The rate and extent of bead capture appeared to decrease nonlinearity with the site density of ICAM-1.

Original languageEnglish (US)
JournalAnnals of Biomedical Engineering
Volume28
Issue numberSUPPL. 1
StatePublished - 2000

ASJC Scopus subject areas

  • Biomedical Engineering

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